| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,735,444 | C→G | 13.4% | R374R (CGG→CGC) ‡ | csdA ← | cysteine desulfurase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,735,444 | 0 | C | G | 13.4% | 77.7 / 6.3 | 30 | R374R (CGG→CGC) ‡ | csdA | cysteine desulfurase |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base G (2/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
ACGCTGTTCCCAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACGAAACTGGCCAGGGCCGTCTGCGG > NC_002947/1735295‑1735593 | aCGCTGTTCCCAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATAcc < 1:203603/150‑1 (MQ=255)aCGCTGTTCCCAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGCCATCGCTGTCGTTGTACAAACCCAGCGATAcc < 2:776671/150‑1 (MQ=255) gCTGTTCCCAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCg > 2:1138381/1‑150 (MQ=255) gCTGTTCCCAGACTTTGCATTATGGGAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCg > 2:1012773/1‑150 (MQ=255) ccAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACGGGTATcgct > 1:636152/1‑149 (MQ=255) cAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACGGGTATcgctg < 2:636152/150‑3 (MQ=255) ttGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTcca < 1:122456/150‑1 (MQ=255) gCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTccagc < 2:203815/150‑1 (MQ=255) gAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCcagccccacccc > 2:369564/1‑150 (MQ=255) tCCAGCGCTTGGTGCGCCCGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTcagca < 2:382719/150‑1 (MQ=255) cAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGt < 2:1190562/150‑1 (MQ=255) aGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGACACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTg > 2:163441/1‑150 (MQ=255) ttGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATg > 1:945252/1‑150 (MQ=255) tGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATgg < 1:543402/150‑1 (MQ=255) cgcCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACGGGTATcgctgg > 2:764773/1‑110 (MQ=255) cgcCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACGGGTATcgctgg < 1:764773/113‑4 (MQ=255) gcCTGTTCCGGAAGGCTCATAGAAACAGCACCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAgt > 2:12923/1‑150 (MQ=255) tCCGGCAGGCTCATCGCAACAGCTCCAGACCTTGGTCCAGCGCGTCGAAGAAACGCTGCCCGTCATCGCTGTCGTGGTACCAACCCGGCGATACCCGTATCGCGCCCTCCAGCCCCCGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGAc < 1:133553/150‑1 (MQ=255) tCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGgcccgcac > 2:516163/1‑150 (MQ=255) tCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTAGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACAGgcacgcac > 1:338875/1‑150 (MQ=255) tCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGt > 1:609019/1‑150 (MQ=255) ccAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCTTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGtt < 1:932381/150‑1 (MQ=255) aGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCCGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTtca < 1:1138381/150‑1 (MQ=255) gcattGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTtcagt > 2:350817/4‑150 (MQ=255) ccTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCCGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTtcagtc < 2:481494/150‑1 (MQ=255) cTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTtcagtca > 2:418859/1‑150 (MQ=255) gaagaaACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATc > 1:78158/1‑150 (MQ=255) cTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATg < 1:418859/150‑1 (MQ=255) tCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACg > 2:1028897/1‑150 (MQ=255) cccAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACGAAACTGGCCAgg < 2:945252/150‑1 (MQ=255) cAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACGAAACTGGCCAGGGc > 2:268598/1‑150 (MQ=255) gCGATACCCGGAACGCGCCCCCAAGCCCCAGCACCTTCAGCAGTGGCATGGCGCCGAGGGGACCGGCACGCACGGCACTGCCCTATGCAGTCAGCAAATGAACAAAATCGGCGTAGTGCACGCACTCGATGACGAAACTGGGCAGTGCCg > 2:382584/1‑150 (MQ=255) ccGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACGAAACTGGCCAGGGCCGTCTGCgg < 1:163441/150‑1 (MQ=255) | ACGCTGTTCCCAGCCTTTGCCCTGTTGAAACGCTTCCAGCGCTTGGTGCGCCTGTTCCGGCAGGCTCATCGCAACAGCTCCAGGCCTTGGTCCAGCGCGTCGAAGAAACGCTGCACGTCATCGCTGTCGTTGTACAAACCCAGCGATACCCGTATCGCGCCCTCCAGCCCCAGCCCCTTCAGCAGTGGCATGGCGCAGTGGTGACCGGCACGCACGGCAATGCCCTGTTCAGTCAGCAAATGAGCAATATCGGCGTTGTGCACGCCCTCGATGACGAAACTGGCCAGGGCCGTCTGCGG > NC_002947/1735295‑1735593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |