Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,851,755 A→G 100% I267V (ATC→GTC)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,7550AG100.0% 99.9 / NA 30I267V (ATC→GTC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/14);  total (16/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATT  >  NC_002947/1851613‑1851898
                                                                                                                                              |                                                                                                                                               
cGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTc                                                                                                                                          >  2:269121/1‑150 (MQ=255)
            gCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCaa                                                                                                                              <  1:234126/150‑1 (MQ=255)
            gCAGATCGCCAAGCTGTCGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCaa                                                                                                                              >  2:802981/1‑150 (MQ=255)
               gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGAt                                                                                                                           >  1:35362/1‑150 (MQ=255)
                                 tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                         <  1:802981/150‑1 (MQ=255)
                                       gcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAg                                                                                                   >  1:547391/1‑150 (MQ=255)
                                           cTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTTCTGACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAg                                                                                               <  1:616792/150‑1 (MQ=255)
                                              gACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATc                                                                                            >  2:233447/1‑150 (MQ=255)
                                                          ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACACGTCTATTCGATCTGGCGCAAGATGCAgc                                                                                                                      >  1:1187851/1‑112 (MQ=255)
                                                          ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACACGTCTATTCGATCTGGCGCAAGATGCAgc                                                                                                                      <  2:1187851/112‑1 (MQ=255)
                                                              tcaGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGGCGTGCgtgcgg                                                                            <  2:547391/150‑1 (MQ=255)
                                                                aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtg                                                                          >  1:817996/1‑150 (MQ=255)
                                                                      gTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTg                                                                    <  2:744786/150‑1 (MQ=255)
                                                                        gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCACGATACAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                  >  2:32848/1‑150 (MQ=255)
                                                                         atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGGCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCCGATCGACGACGTGCGCGCGGTGCGCGTGCTg                                                                 <  1:32848/150‑1 (MQ=255)
                                                                         atgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCtt                                                                 <  1:233447/150‑2 (MQ=255)
                                                                                    gCAGAACGAGTCGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAAt                                                      >  2:609552/1‑150 (MQ=255)
                                                                                       gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                  <  2:711726/135‑1 (MQ=255)
                                                                                       gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                  >  1:711726/1‑135 (MQ=255)
                                                                                                  tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGAt                                                                                             <  2:122863/97‑1 (MQ=255)
                                                                                                  tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGAt                                                                                             >  1:122863/1‑97 (MQ=255)
                                                                                                  tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATACGCGa                                                <  2:144209/142‑1 (MQ=255)
                                                                                                  tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATACGCGa                                                >  1:144209/1‑142 (MQ=255)
                                                                                                             gTGAAGGCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTTCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGc                             >  1:197887/1‑150 (MQ=255)
                                                                                                                   gCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtgc                                                                         >  1:383944/1‑100 (MQ=255)
                                                                                                                   gCCGACATCAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtgc                                                                         <  2:383944/100‑1 (MQ=255)
                                                                                                                         tgcAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACAcc                 >  1:948197/3‑150 (MQ=255)
                                                                                                                           cAGCGGCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCtt               >  1:497832/1‑150 (MQ=255)
                                                                                                                               ggCCGGGCGAAACATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTgg           <  2:35362/150‑1 (MQ=255)
                                                                                                                                        aaaCATGTCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATAtt  <  2:948197/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATT  >  NC_002947/1851613‑1851898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑