| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,863,282 | T→A | 9.1% | L309Q (CTG→CAG) | PP_1666 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,863,282 | 0 | T | A | 9.1% | 153.9 / 6.1 | 55 | L309Q (CTG→CAG) | PP_1666 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/20); new base A (3/2); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAG > NC_002947/1863140‑1863425 | gTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAg > 2:700231/1‑150 (MQ=255) cAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGAGAACAGCTACGTGCCCAGCTTGGCCAGGCCAAGCTg < 2:87716/150‑1 (MQ=255) ccTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGcc > 1:1108570/1‑150 (MQ=255) tGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCgg > 1:32991/1‑150 (MQ=255) tGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCgg > 2:395220/1‑150 (MQ=255) gcTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGc > 1:678889/1‑150 (MQ=255) gcTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGc < 2:535322/150‑1 (MQ=255) tATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAg < 2:1145871/150‑1 (MQ=255) ccGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCAc < 1:395220/150‑1 (MQ=255) gCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggc > 1:319529/1‑150 (MQ=255) tGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTAAATTGCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggcgg > 1:12730/1‑150 (MQ=255) cgcgTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCg < 2:1108570/150‑1 (MQ=255) cTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggc > 1:340723/1‑137 (MQ=255) cTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggc < 2:340723/137‑1 (MQ=255) cTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑Tg > 2:424501/1‑150 (MQ=255) gAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCa < 1:700231/150‑1 (MQ=255) tATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctg < 2:582473/90‑1 (MQ=255) tATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctg > 1:582473/1‑90 (MQ=255) aTGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCgg < 2:678889/150‑1 (MQ=255) ccACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTg > 2:278669/1‑150 (MQ=255) ccACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTg > 2:707435/1‑150 (MQ=255) aCGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGa > 1:1147189/1‑150 (MQ=255) cTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCa < 2:1005836/133‑1 (MQ=255) cTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCa > 1:1005836/1‑133 (MQ=255) ttGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCg > 2:138076/1‑123 (MQ=255) ttGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCg < 1:138076/123‑1 (MQ=255) cAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCa < 1:424501/150‑1 (MQ=255) aGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGcc < 2:999113/118‑1 (MQ=255) aGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGcc > 1:999113/1‑118 (MQ=255) aGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAg > 1:906402/1‑150 (MQ=255) gcggcTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGcc > 2:1172345/1‑150 (MQ=255) tGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCa > 1:204752/1‑150 (MQ=255) ggCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGccc > 2:586630/1‑150 (MQ=255) gCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctgg < 2:681373/63‑1 (MQ=255) gCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctgg > 1:681373/1‑63 (MQ=255) gCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAg < 2:907722/89‑1 (MQ=255) gCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAg > 1:907722/1‑89 (MQ=255) aCCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCt > 2:444838/1‑150 (MQ=255) ggggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCcgcg > 1:222564/1‑126 (MQ=255) ggggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCcgcg < 2:222564/126‑1 (MQ=255) gggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAc < 1:1172345/150‑1 (MQ=255) gggCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTATCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGcc > 1:591368/1‑150 (MQ=255) gggCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCcg < 1:881544/116‑1 (MQ=255) gggCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCcg > 2:881544/1‑116 (MQ=255) gggCAATCGTGAACAGCTAAGTGCCCAGATTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGTCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCCGCACCCAAGcc > 2:409183/1‑150 (MQ=255) gTGAACAGCTACGTGCCCAGCTTGGCCGGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTATCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACg < 2:591368/150‑1 (MQ=255) aGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGACGACAGGGTTGGTGCCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGc < 2:938049/150‑1 (MQ=255) cTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTACCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGt > 2:120541/1‑150 (MQ=255) cGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGtttt < 1:278669/150‑1 (MQ=255) cGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTACCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGtttt < 1:120541/150‑1 (MQ=255) tgcAGCTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTACCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCt > 2:334414/3‑150 (MQ=255) cccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCt < 1:586630/150‑1 (MQ=255) gcAGCTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTg > 2:999878/2‑150 (MQ=255) cAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTgg > 1:950329/1‑150 (MQ=255) cAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACAGGCAGTTGACGCCGAAGAAGAGCAGAAGGGGATTGGGCTGCGATCACCCAAGTCGTTCGACGGCTTGCGTTTTCGCTgg > 1:570961/1‑150 (MQ=255) cTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGt > 1:869033/1‑74 (MQ=255) cTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGt < 2:869033/74‑1 (MQ=255) ttGGCCTTGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCAAAAGAAG‑ACATGCCAGTTGCAGAACATTCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAg < 1:444838/150‑1 (MQ=255) ttGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAg > 2:983557/1‑150 (MQ=255) gcctggccAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCa > 1:1173522/1‑59 (MQ=255) gcctggccAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCa < 2:1173522/59‑1 (MQ=255) cctggccAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑t < 2:407412/54‑1 (MQ=255) cctggccAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑t > 1:407412/1‑54 (MQ=255) | GTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCA‑TGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAG > NC_002947/1863140‑1863425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |