Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,864,795 G→A 7.7% A29A (GCG→GCA)  hda → DnaA regulatory inactivator Hda

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,864,7950GA 7.7% 147.1 / 3.0 52A29A (GCG→GCA) hdaDnaA regulatory inactivator Hda
Reads supporting (aligned to +/- strand):  ref base G (22/26);  new base A (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

GTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGA  >  NC_002947/1864648‑1864932
                                                                                                                                                   |                                                                                                                                         
gTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGc                                                                                                                                         >  2:86573/1‑150 (MQ=255)
      aCTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACTCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTggg                                                                                                                                   <  1:546602/150‑1 (MQ=255)
       cTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGACTGCGCGAAGACGCCAGCCTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGt                                                                                                                                  >  1:271414/1‑150 (MQ=255)
             aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                            <  2:24074/150‑1 (MQ=255)
             aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                            >  2:177257/1‑150 (MQ=255)
             aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggc                                                                                                                                   <  2:686038/143‑2 (MQ=255)
             aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggc                                                                                                                                   >  1:686038/1‑142 (MQ=255)
             aGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCATGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTc                                                                                                                            >  1:586985/1‑150 (MQ=255)
                 tGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGc                                                                                                                        >  2:1074440/1‑150 (MQ=255)
                      aCCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTa                                                                                                                   >  1:913779/1‑150 (MQ=255)
                       ccAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTAt                                                                                                                  >  1:461942/1‑150 (MQ=255)
                        cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATg                                                                                                                 >  1:525091/1‑150 (MQ=255)
                        cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATg                                                                                                                 >  1:590473/1‑150 (MQ=255)
                        cAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATg                                                                                                                 <  1:219875/150‑1 (MQ=255)
                              cccccAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCATGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGc                                                                                                           <  2:586985/150‑1 (MQ=255)
                               ccccAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGcc                                                                                                          >  2:223847/1‑150 (MQ=255)
                                        tCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCCGGGCGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGc                                                                                                 >  2:315296/1‑150 (MQ=255)
                                          ccGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTg                                                                                               >  2:948801/1‑150 (MQ=255)
                                                aCCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCga                                                                                         <  2:431660/150‑1 (MQ=255)
                                                aCCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGACTATGCGAAGCCGACGCCGGCTGGACCga                                                                                         <  1:905733/150‑1 (MQ=255)
                                                   gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggc                                                                                                                                   >  1:941245/1‑104 (MQ=255)
                                                   gcccgcGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCCGCAGCATTggc                                                                                                                                   <  2:941245/105‑2 (MQ=255)
                                                      cgcgAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCt                                                                                   <  1:177257/150‑1 (MQ=255)
                                                            cATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTa                                                                             <  1:1074440/150‑1 (MQ=255)
                                                             aTGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTAc                                                                            <  2:362793/150‑1 (MQ=255)
                                                               gAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTATTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCt                                                                          >  2:1139643/1‑150 (MQ=255)
                                                                 aaCCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCtct                                                                        <  1:948801/150‑3 (MQ=255)
                                                                   ccaccGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGGGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTgg                                                                      <  2:661535/150‑1 (MQ=255)
                                                                     accGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTgggg                                                                    >  1:1038751/1‑150 (MQ=255)
                                                                       cGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCa                                                                  >  1:2577/1‑150 (MQ=255)
                                                                          tCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGCCCAAGGCCTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGc                                                               <  1:968583/150‑1 (MQ=255)
                                                                            cAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAg                                                                                                            >  1:467128/1‑103 (MQ=255)
                                                                            cAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAg                                                                                                            <  2:467128/103‑1 (MQ=255)
                                                                             aGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAgg                                                            >  1:848751/1‑150 (MQ=255)
                                                                             aGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAgg                                                            <  1:86573/150‑1 (MQ=255)
                                                                              gTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAggg                                                           >  2:426843/1‑150 (MQ=255)
                                                                                   cccTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATATAACTCAGGGGCAAGAAGGgtgtgg                                                      >  2:68030/1‑148 (MQ=255)
                                                                                     cTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGAAGGGTGTTGGc                                                    >  1:956952/1‑150 (MQ=255)
                                                                                              cgcCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCAc                                           <  2:1038751/150‑1 (MQ=255)
                                                                                               gcCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGcgg                                                                                               >  1:292757/1‑95 (MQ=255)
                                                                                               gcCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGcgg                                                                                               <  2:292757/97‑3 (MQ=255)
                                                                                                   gcgcGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTCCGGCATTGGGTTACGTCAAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGCCAAGCAGGGTGTTGGCCGTTCCCACctgct                                      >  2:478698/1‑150 (MQ=255)
                                                                                                      cgATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCa                                   <  2:1028535/150‑1 (MQ=255)
                                                                                                      cgATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCa                                   <  2:271414/150‑1 (MQ=255)
                                                                                                            cGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGgccgc                             <  2:848751/150‑1 (MQ=255)
                                                                                                               cACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCAc                          <  2:461942/150‑1 (MQ=255)
                                                                                                                  cTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCa                       <  2:525091/150‑1 (MQ=255)
                                                                                                                          actattATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTcc               <  1:1139643/150‑1 (MQ=255)
                                                                                                                           ctactaTCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCa              <  1:426843/150‑1 (MQ=255)
                                                                                                                                    gggCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTgg     <  1:946010/150‑1 (MQ=255)
                                                                                                                                    gggCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTgg     <  1:223847/150‑1 (MQ=255)
                                                                                                                                       cgcCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGa  <  2:956952/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
GTATAGACTTTGCAGATTGTTCACCAAAGGCCCCCAGCGGTCCCGTCGACCGCCCGCGAGCATGAAACCACCGATCCAGTTGCCCCTGGGTGTGCGCCTGCGCGATGACGCCACCTTCATCAACTACTATCCGGGCGCCAATGCTGCGGCATTGGGTTACGTCGAGCGGCTATGCGAAGCCGACGCCGGCTGGACCGAGAGCCTTATCTACCTGTGGGGCAAGCAGGGTGTTGGCCGTTCCCACCTGCTGCAGGCCGCCACCCATCGCTTCCAGCAACGTGGCGA  >  NC_002947/1864648‑1864932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑