Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,912,464	C→T	6.3%	R350R (CGG→CGA)	PP_1712 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,912,464	0	C	T	6.3%	187.5 / 2.2	65	R350R (CGG→CGA)	PP_1712	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base T (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

AGCGCACTGCCAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAGTGGTGGCCCACCTCGCCGAAT  >  NC_002947/1912316‑1912605                                                                                                                                                       |                                                                                                                                              aGCGCACTGCCAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGcc                                                                                                                                              <  1:715401/150‑1 (MQ=255)    gcACTGCCAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGt                                                                                                                                           >  1:1168020/1‑150 (MQ=255)        tGCCAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTagag                                                                                                                                       >  2:732714/1‑150 (MQ=255)         ggcAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGCCTCACCAGCATAGTCCAGCCGGTAGAgg                                                                                                                                      >  2:600147/3‑150 (MQ=255)          ccAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAggg                                                                                                                                     >  1:1155080/1‑150 (MQ=255)            aGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGta                                                                                                                                   >  1:440650/1‑149 (MQ=255)                tGAAGTACAAGAACCCTCCGTACCCGTCAACCCCGCCCTTGGTTTCTTCAATATGGGACCACCCGTCCAGACG‑TG‑TGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGtt                                                                                                                               <  1:1113813/150‑1 (MQ=255)                     tAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTg                                                                                                                          >  1:832135/1‑150 (MQ=255)                     tAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACACGAGAGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTg                                                                                                                          >  1:734588/1‑150 (MQ=255)                      aaaaGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGc                                                                                                                         <  1:598316/150‑1 (MQ=255)                       aaaGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCg                                                                                                                        >  2:86473/1‑150 (MQ=255)                        aaGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGcc                                                                                                                                              <  2:775523/127‑1 (MQ=255)                        aaGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGcc                                                                                                                                              >  1:775523/1‑127 (MQ=255)                           aaaCCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcg                                                                                                                    >  2:1189521/1‑150 (MQ=255)                             accaccGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtc                                                                                                                  <  1:86473/150‑1 (MQ=255)                               caccGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtcgt                                                                                                                <  2:804669/150‑1 (MQ=255)                                       ccGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGag                                                                                                        <  2:987276/150‑1 (MQ=255)                                            tAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTg                                                                                                   <  1:467088/150‑1 (MQ=255)                                             aGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGc                                                                                                  <  1:724501/150‑1 (MQ=255)                                               cccccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtcgt                                                                                                                >  1:505758/1‑134 (MQ=255)                                               cccccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtcgt                                                                                                                <  2:505758/134‑1 (MQ=255)                                               cccccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTg                                                                                                <  1:460612/150‑1 (MQ=255)                                                 cccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAACCGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGc                                                                                              >  1:81763/1‑150 (MQ=255)                                                  ccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGcc                                                                                             >  1:53477/1‑150 (MQ=255)                                                  ccccTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGcc                                                                                             >  1:540814/1‑150 (MQ=255)                                                     cTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTg                                                                                                                              <  2:426324/114‑1 (MQ=255)                                                     cTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTg                                                                                                                              >  1:426324/1‑114 (MQ=255)                                                     cTCGGTTTCTTCAATAAGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTa                                                                                          <  2:217220/150‑1 (MQ=255)                                                      tCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTAc                                                                                         <  2:81763/150‑1 (MQ=255)                                                              ttcAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGAt                                                                                 >  2:662648/1‑150 (MQ=255)                                                                  atatGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGTCGGTAGAGGGTCGGTTGGCTGCGATCGTCGtcgatc                                                                                                           <  1:507981/120‑1 (MQ=255)                                                                  atatGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGTCGGTAGAGGGTCGGTTGGCTGCGATCGTCGtcgatc                                                                                                           <  1:507922/120‑1 (MQ=255)                                                                  atatGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGTCGGTAGAGGGTCGGTTGGCTGCGATCGTCGtcgatc                                                                                                           >  2:507981/1‑120 (MQ=255)                                                                  atatGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGTCGGTAGAGGGTCGGTTGGCTGCGATCGTCGtcgatc                                                                                                           >  2:507922/1‑120 (MQ=255)                                                                  atatGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGt                                                                             <  2:832135/150‑1 (MQ=255)                                                                      gggACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGgc                                                                         <  2:540814/150‑1 (MQ=255)                                                                        gACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTCGTTGAACTCCCGGCTTCGCCAGCCCGCATAGCCCTGGCTGTATTGGGTCGGGGTTCTGCGGTCGGCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGgctg                                                                       <  2:1041638/150‑1 (MQ=255)                                                                         accaccCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGtt                                                                            <  2:1156145/144‑1 (MQ=255)                                                                         accaccCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGtt                                                                            >  1:1156145/1‑144 (MQ=255)                                                                            accCG‑CC‑AGCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGt                                                                   >  1:411563/1‑150 (MQ=255)                                                                                         aTGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTg                                                        >  2:418683/1‑150 (MQ=255)                                                                                              gAAACGCACTACCTGCCCCTGGTTGAACTCGAGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtcgt                                                                                                                <  1:1033074/89‑1 (MQ=255)                                                                                              gAAACGCACTACCTGCCCCTGGTTGAACTCGAGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGAtcgtcgt                                                                                                                >  2:1033074/1‑89 (MQ=255)                                                                                                         ccTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGTGTTGGCTTCTGTCTTCTACCGTGAAGCCGCTATtgatga                                        <  2:440650/150‑1 (MQ=255)                                                                                                         ccTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTAt                                              <  2:173468/144‑1 (MQ=255)                                                                                                         ccTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTAt                                              >  1:173468/1‑144 (MQ=255)                                                                                                           tGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTgg                                                                                                                             <  2:968475/63‑1 (MQ=255)                                                                                                           tGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTgg                                                                                                                             >  1:968475/1‑63 (MQ=255)                                                                                                               ccTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGATATTGATGATCCCtt                                  >  1:296250/1‑150 (MQ=255)                                                                                                                  ggTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGt                               <  2:209782/150‑1 (MQ=255)                                                                                                                  ggTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGt                               <  1:173269/150‑1 (MQ=255)                                                                                                                       aaCTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGAc                          <  1:1189521/150‑1 (MQ=255)                                                                                                                          tCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGAc                          <  1:100475/147‑1 (MQ=255)                                                                                                                          tCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGAc                          >  2:100475/1‑147 (MQ=255)                                                                                                                            gcgGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAgtg                     <  2:1155080/150‑1 (MQ=255)                                                                                                                              gggCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGA‑CAgtggt                   >  1:707887/1‑149 (MQ=255)                                                                                                                              gggCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGA‑CAgtggt                   <  2:707887/149‑1 (MQ=255)                                                                                                                               ggCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGagta                                                                                                      <  2:594228/66‑1 (MQ=255)                                                                                                                               ggCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGagta                                                                                                      >  1:594228/1‑66 (MQ=255)                                                                                                                                  aGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAGTGGTGGcc               <  1:732714/150‑1 (MQ=255)                                                                                                                                    gCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTg                                                                           <  2:530753/88‑1 (MQ=255)                                                                                                                                    gCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTg                                                                           >  1:530753/1‑88 (MQ=255)                                                                                                                                    gCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAGTGGTGGCCCa             >  2:286716/1‑150 (MQ=255)                                                                                                                                        gCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGt                               <  1:155449/128‑1 (MQ=255)                                                                                                                                        gCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGt                               >  2:155449/1‑128 (MQ=255)                                                                                                                                               tAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCAATCGTAGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAGTGGTGGCCCACCTCGCCGAAt  <  2:862278/150‑1 (MQ=255)                                                                                                                                                    cAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATtga                                           <  1:507322/104‑1 (MQ=255)                                                                                                                                                    cAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATtga                                           >  2:507322/1‑104 (MQ=255)                                                                                                                                                       |                                                                                                                                              AGCGCACTGCCAGGTTGAAGTAAAAGAAACCACCGTACCCGATTAGCCCCCCCTCGGTTTCTTCAATATGGGACCACCCG‑CC‑GTCGATGCCGAAACGCACTACCTGCCCCTGGTTGAACTCGCGGGCAGGCTCGCCAGCATAGTCCAGCCGGTAGAGGGTCGGTTGGCTGCGATCGTCGTCGATCGAGTAGTGCTGGCCGTACAACGTGATGCGTTGGCTGCTGTCTTCTACCGTGAAGCCGCTATTGATGATCCCTTCGTCTGACCAGTGGTGGCCCACCTCGCCGAAT  >  NC_002947/1912316‑1912605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑