Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,921,591 T→A 10.5% intergenic (+24/+37) PP_1720 → / ← PP_1721 alcohol dehydrogenase/phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,921,5910TA10.5% 162.9 / 9.6 57intergenic (+24/+37)PP_1720/PP_1721alcohol dehydrogenase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (20/31);  new base A (3/3);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCA  >  NC_002947/1921444‑1921731
                                                                                                                                                   |                                                                                                                                            
gCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTa                                                                                                                                            >  2:803486/1‑150 (MQ=255)
 ggtttGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGTCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCTCGGGTTTAc                                                                                                                                           <  2:1152472/147‑1 (MQ=255)
 cGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAc                                                                                                                                           <  1:654180/150‑1 (MQ=255)
        cgccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGaa                                                                                                                                    <  2:960973/150‑1 (MQ=255)
         gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAg                                                                                                                                   >  2:465015/1‑150 (MQ=255)
          ccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAgg                                                                                                                                  <  1:1108596/150‑1 (MQ=255)
           cgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGGTCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAggg                                                                                                                                 <  1:515819/150‑1 (MQ=255)
           cgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTAAACCCGCGAAggg                                                                                                                                 <  1:499125/150‑1 (MQ=255)
            gcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAgggg                                                                                                                                <  2:60096/150‑1 (MQ=255)
            gcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAgggg                                                                                                                                <  1:465015/150‑1 (MQ=255)
                 tGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGc                                                                                                                           >  2:451556/1‑150 (MQ=255)
                         acCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCa                                                                                                                   <  1:1208064/150‑1 (MQ=255)
                           cTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATa                                                                                                                 >  2:171776/1‑150 (MQ=255)
                              tCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAg                                                                                                                                   <  2:211603/129‑1 (MQ=255)
                              tCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAg                                                                                                                                   >  1:211603/1‑129 (MQ=255)
                              tCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATaaaa                                                                                                              <  2:521994/150‑1 (MQ=255)
                                 ggTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAcc                                                                                                           <  1:194146/150‑1 (MQ=255)
                                   tGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCtt                                                                                                         <  1:171776/150‑1 (MQ=255)
                                     gAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                       <  2:621688/150‑1 (MQ=255)
                                             ccAGGCGGCGTATGCCGAACTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcagctccagc                                                                                               >  1:1185637/1‑150 (MQ=255)
                                                    gcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAc                                                                                                            >  2:552681/1‑130 (MQ=255)
                                                    gcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAc                                                                                                            <  1:552681/130‑1 (MQ=255)
                                                    gcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGt                                                                                        >  1:901994/1‑150 (MQ=255)
                                                           ccGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                 >  2:954856/1‑150 (MQ=255)
                                                                       ccaaccaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGATCCAGCTCAGACTCTGTAACCCATTGATCTCg                                                                     <  2:676759/150‑1 (MQ=255)
                                                                          accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                  >  1:410567/1‑134 (MQ=255)
                                                                          accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCa                                                                                  <  2:410567/134‑1 (MQ=255)
                                                                          accaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCa                                                                  <  1:451556/150‑1 (MQ=255)
                                                                               gtgtCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAACAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                          <  1:977051/137‑1 (MQ=255)
                                                                               gtgtCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGa                                                                          >  2:977051/1‑137 (MQ=255)
                                                                                 gtCGGGGAAGCGGGTGATGGTGATCGACGCGAGCCGGGTGTAAGCGGGGCTGGCCCGTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTcgc                                                           <  2:87286/150‑1 (MQ=255)
                                                                                     ggCAAGCTGGTGATGGTGATCGACCCTAGCTCGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTCCCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCaa                                                       <  1:39435/150‑1 (MQ=255)
                                                                                      gCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCCGGCCCCTTCGCGGGTTTACCCGCGAAgg                                                                                                                                  >  2:930125/1‑74 (MQ=255)
                                                                                      gCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCCGGCCCCTTCGCGGGTTTACCCGCGAAgg                                                                                                                                  <  1:930125/74‑1 (MQ=255)
                                                                                                     tgatCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTc                                       <  2:657044/150‑1 (MQ=255)
                                                                                                             ccTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                 >  1:1035761/1‑100 (MQ=255)
                                                                                                             ccTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCAc                                                                                 <  2:1035761/100‑1 (MQ=255)
                                                                                                                aGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                            <  1:803486/150‑1 (MQ=255)
                                                                                                                 gCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                           <  2:1185637/150‑1 (MQ=255)
                                                                                                                         tAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCTGGTTAAGAACATGCGGGCGATCTAc                   >  2:450316/1‑150 (MQ=255)
                                                                                                                               gggCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                           >  1:417570/1‑136 (MQ=255)
                                                                                                                               gggCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                           <  2:417570/136‑1 (MQ=255)
                                                                                                                               gggCTGGCCCCTTCGCGGCTTTCCCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATCGATATCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaa             <  1:450316/150‑1 (MQ=255)
                                                                                                                                 gCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGTCCACCCATCGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaaca           >  2:226049/1‑150 (MQ=255)
                                                                                                                                   cggCCCCTTCGCGGGTTTCCCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTcaacaac         <  1:387245/149‑1 (MQ=255)
                                                                                                                                     gCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                           <  2:51186/114‑1 (MQ=255)
                                                                                                                                     gCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                           >  1:51186/1‑114 (MQ=255)
                                                                                                                                      ccccTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGTCCACCCATCGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac      <  1:226049/150‑1 (MQ=255)
                                                                                                                                         cTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                           >  2:654005/1‑126 (MQ=255)
                                                                                                                                         cTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                           <  1:654005/126‑1 (MQ=255)
                                                                                                                                         cTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGGTCACCACATGCGGGTGATCCACTCTCAACAACAACGGc   >  1:855351/1‑150 (MQ=255)
                                                                                                                                         cTTCGCGGGTAAACCCGCGAAGGGGCCGGCATAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGc   >  1:686191/1‑150 (MQ=255)
                                                                                                                                          ttCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCa  >  2:1197774/1‑150 (MQ=255)
                                                                                                                                          ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGAACTCAGCAt                                                                 >  1:369158/1‑87 (MQ=38)
                                                                                                                                          ttCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGAACTCAGCAt                                                                 <  2:369158/87‑1 (MQ=38)
                                                                                                                                          ttCGCGGGTAAACCCGCGAAGGGGCCAGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                           >  2:69921/1‑109 (MQ=39)
                                                                                                                                          ttCGCGGGTAAACCCGCGAAGGGGCCAGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                           <  1:69921/109‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCA  >  NC_002947/1921444‑1921731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑