| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,967,690 | C→G | 13.4% | G115R (GGC→CGC) ‡ | yciK ← | putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,967,690 | 0 | C | G | 13.4% | 120.4 / 12.2 | 45 | G115R (GGC→CGC) ‡ | yciK | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (21/18); new base G (3/3); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
GCCCCAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGATGACCACC > NC_002947/1967541‑1967838 | gcCCCAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGcc > 1:472281/1‑150 (MQ=255) ccccAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTTAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGccc < 2:823580/150‑1 (MQ=255) gTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACaa < 1:594980/150‑1 (MQ=255) ccGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTc > 1:171984/1‑150 (MQ=255) ccccACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTgcg > 2:289252/1‑150 (MQ=255) tgctgctgGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCga < 1:302560/150‑1 (MQ=255) gTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGAc > 2:946133/1‑150 (MQ=255) acacCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAt < 1:946133/150‑1 (MQ=255) caccGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt > 2:584685/1‑150 (MQ=255) accGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCg < 2:75666/132‑1 (MQ=255) accGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCg > 1:75666/1‑132 (MQ=255) cGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGGTGAg < 2:599228/150‑1 (MQ=255) aGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAg < 2:171984/150‑1 (MQ=255) cGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGGCGACAACttca < 2:478612/98‑4 (MQ=255) cGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGGCGACAACttca > 1:478612/1‑95 (MQ=255) aGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAggcgg < 2:1018873/150‑1 (MQ=255) aGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGCCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAggcgg > 1:1191139/1‑150 (MQ=255) gcagcGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACt > 1:1078542/1‑150 (MQ=255) cagcGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCgg < 2:651535/102‑1 (MQ=255) cagcGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCgg > 1:651535/1‑102 (MQ=255) ttcagcagGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCt > 2:777392/3‑150 (MQ=255) agcagGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCg > 2:631391/1‑150 (MQ=255) gctGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGgccgc < 1:359607/148‑1 (MQ=255) tGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtc > 1:413622/1‑142 (MQ=255) tGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtc < 2:413622/142‑1 (MQ=255) ttGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGcc < 2:1113948/68‑1 (MQ=255) ttGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGcc > 1:1113948/1‑68 (MQ=255) gACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTgg < 2:798787/150‑1 (MQ=255) aTGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCg > 1:370971/1‑150 (MQ=255) gtgCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGt < 2:370971/150‑1 (MQ=255) gCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGttt > 2:1063494/1‑150 (MQ=255) cATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCa < 1:44239/77‑1 (MQ=255) cATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCa > 2:44239/1‑77 (MQ=255) gcgcATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGtt > 1:7224/1‑150 (MQ=255) gcATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCgg > 1:1105197/1‑138 (MQ=255) gcATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCgg < 2:1105197/138‑1 (MQ=255) cATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGaa > 2:923405/1‑150 (MQ=255) cATGAAGTTGTCGCCCGACAACTGATCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGCGCAGGCCATCTAGGCGGCCGAACTGGTCTTCGATCCTCAGCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTCACAGGTTGac < 2:472281/150‑2 (MQ=255) gcaGTTGTCGGGCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGtt < 2:524616/134‑1 (MQ=255) gcaGTTGTCGGGCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGtt > 1:524616/3‑136 (MQ=255) gcaGTTGTCGGGCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAg < 2:261326/139‑1 (MQ=255) gcaGTTGTCGGGCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAg > 1:261326/3‑141 (MQ=255) aGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGAGGGCCGATGATAGACTTTTTGTTGAGCAGGCCATCTTGACGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGa > 1:511289/1‑150 (MQ=255) tttGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATg < 1:1172854/96‑1 (MQ=255) gTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATg > 2:1172854/1‑97 (MQ=255) ttGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGATTCCAGGTTGAACGGGATg > 1:739746/1‑150 (MQ=255) gTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGATGac < 1:923405/150‑1 (MQ=255) cccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGATGaccacc > 2:1050611/1‑150 (MQ=255) cccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTCGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGATGaccacc > 2:518685/1‑150 (MQ=255) ccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGtt < 1:836658/133‑1 (MQ=255) ccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGtt > 2:836658/1‑133 (MQ=255) | GCCCCAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCATTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGATGACCACC > NC_002947/1967541‑1967838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |