Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,972,518 C→T 7.8% T468T (ACC→ACT)  gyrA → DNA gyrase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,972,5180CT 7.8% 144.4 / 3.3 51T468T (ACC→ACT) gyrADNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CCGTGCAGGTCATGGTCGAGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGCCATTCGTGCCGAGTACGGCGATGT  >  NC_002947/1972373‑1972664
                                                                                                                                                 |                                                                                                                                                  
ccGTGCAGGTCATGGTCGAGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACTGGcc                                                                                                                                                >  1:771662/1‑150 (MQ=255)
ccGTGCAGGTCATGGTCGAGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACTGGcc                                                                                                                                                >  1:771656/1‑150 (MQ=255)
                 gAGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAgctg                                                                                                                               <  2:353579/150‑1 (MQ=255)
                  aGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCCGGTGCCTCCCCAGCTGCGCCTGCACCGCTTGACCGGCCTGGAGCACGAGAAgctgc                                                                                                                              <  1:151492/150‑1 (MQ=255)
                     gcgccggcgccgATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTgg                                                                                                                           <  2:185909/150‑1 (MQ=255)
                         cggcgccgATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGa                                                                                                                       >  1:95103/1‑150 (MQ=255)
                             gcggATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTAc                                                                                                                   >  1:1203665/4‑150 (MQ=255)
                                     cTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAgc                                                                                                                                 >  2:418092/1‑128 (MQ=255)
                                     cTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAgc                                                                                                                                 <  1:418092/128‑1 (MQ=255)
                                      tGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATc                                                                                                          >  1:240222/1‑150 (MQ=255)
                                         cgccCTGAGGACTTGCCGCAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTc                                                                                                       <  1:61291/150‑1 (MQ=255)
                                           cccTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGa                                                                                                     <  1:218595/150‑1 (MQ=255)
                                              tGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCa                                                                                                  >  2:579201/1‑150 (MQ=255)
                                                  gACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGAtt                                                                                              <  1:480908/150‑1 (MQ=255)
                                                    ccTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTAACAgg                                                                                                               >  1:1100253/1‑131 (MQ=255)
                                                    ccTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTAACAgg                                                                                                               <  2:1100253/131‑1 (MQ=255)
                                                     cTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACTGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGc                                                                                           <  2:771656/150‑1 (MQ=255)
                                                     cTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACTGGCAAGAAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGc                                                                                           <  2:771662/150‑1 (MQ=255)
                                                     cTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGc                                                                                           <  1:443550/150‑1 (MQ=255)
                                                      tGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCg                                                                                          >  1:608823/1‑150 (MQ=255)
                                                       gCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGa                                                                                         <  1:579201/150‑1 (MQ=255)
                                                                 tACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGAGCCGc                                                                               >  1:296801/1‑150 (MQ=255)
                                                                      ccTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCt                                                                          >  1:834038/1‑150 (MQ=255)
                                                                               cgGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAgcgc                                                                 <  2:25052/150‑1 (MQ=255)
                                                                                ggCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGc                                                                                           <  2:271840/123‑1 (MQ=255)
                                                                                ggCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGc                                                                                           >  1:271840/1‑123 (MQ=255)
                                                                                ggCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGcc                                                                >  2:404892/1‑150 (MQ=255)
                                                                                  cAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGa                                                              >  1:161454/1‑150 (MQ=255)
                                                                                   aaGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTcagca                                                                     <  2:483346/142‑1 (MQ=255)
                                                                                   aaGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTcagca                                                                     >  1:483346/1‑142 (MQ=255)
                                                                                       aCTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGcc                                                         <  2:1195196/150‑1 (MQ=255)
                                                                                         ttCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGa                                                              <  2:483070/143‑1 (MQ=255)
                                                                                         ttCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGa                                                              >  1:483070/1‑143 (MQ=255)
                                                                                          tCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGa                                                      <  2:834038/150‑1 (MQ=255)
                                                                                           ccTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAAACTTCGTGCACAATCGCGAGTTTATCCGCATCCTCAGCAGCGCCGAGCGCCTGAt                                                     <  1:453979/150‑1 (MQ=255)
                                                                                            cTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATg                                                    <  2:159204/150‑1 (MQ=255)
                                                                                              gTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGTAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGAtt                                                                                              >  2:926391/1‑106 (MQ=255)
                                                                                              gTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGTAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGAtt                                                                                              <  1:926391/106‑1 (MQ=255)
                                                                                                 gCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGt                                               >  2:405194/1‑150 (MQ=255)
                                                                                                    ggAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGAt                                            <  1:405194/150‑1 (MQ=255)
                                                                                                      aaCAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGACGGAAGTGATTc                                          <  2:240222/150‑1 (MQ=255)
                                                                                                        caggcccaggccATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTcgc                                        >  2:229709/1‑150 (MQ=255)
                                                                                                             ccaggccATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAg                                                <  2:536044/137‑1 (MQ=255)
                                                                                                             ccaggccATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAg                                                >  1:536044/1‑137 (MQ=255)
                                                                                                             ccaggccATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCgagga                                   <  1:404892/150‑1 (MQ=255)
                                                                                                                   cATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGa                             >  1:204362/1‑150 (MQ=255)
                                                                                                                    aTTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAg                            >  2:494133/1‑150 (MQ=255)
                                                                                                                       ctcGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGcc                         >  1:558758/1‑150 (MQ=255)
                                                                                                                                       aCCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGCCATTCGTGCCGAGTACg         >  2:51035/1‑150 (MQ=255)
                                                                                                                                          gCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGCCATTCGTGCCGAGTACGGCg      <  2:608823/150‑1 (MQ=255)
                                                                                                                                              gACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGCCATTCCTGCCGAGTACGGCGAtgt  <  2:709879/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCGTGCAGGTCATGGTCGAGCGCGCCGGCGCCGATTCCTGCCGCCCTGAGGACCTGCCGGAGCAGTACGGCCTGCACGACGGCAAGTACTTCCTGTCGCCGGAACAGGCCCAGGCCATTCTCGACCTGCGCCTGCACCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTGGCCGAGTACCAGGAAATCCTCGAGCAGATTGGCGAGTTGATCCGCATCCTCAGCAGCGCCGAGCGCCTGATGGAAGTGATTCGCGAGGAGCTGGAGGCCATTCGTGCCGAGTACGGCGATGT  >  NC_002947/1972373‑1972664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑