Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,973,914 A→T 9.4% intergenic (+28/‑120) gyrA → / → serC DNA gyrase subunit A/3‑phosphoserine/phosphohydroxythreonine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,973,9140AT 9.4% 157.1 / 7.4 53intergenic (+28/‑120)gyrA/serCDNA gyrase subunit A/3‑phosphoserine/phosphohydroxythreonine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base T (3/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

AGCCGTCCGAGGACGAACTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGCCTTTAACTTCTGCGC  >  NC_002947/1973768‑1974062
                                                                                                                                                  |                                                                                                                                                    
aGCCGTCCGAGGACGAACTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAgg                                                                                                                                                   <  1:1194589/150‑1 (MQ=255)
 gCCGTCCGAGGACGAACTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGt                                                                                                                                                  <  1:1206817/150‑1 (MQ=255)
         aGGACGAACTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGt                                                                                                                                          <  2:986664/150‑1 (MQ=255)
                aCTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACtg                                                                                                                                   >  1:67487/1‑150 (MQ=255)
                   cGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTgtgt                                                                                                                                >  1:744218/1‑150 (MQ=255)
                    gATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTa                                                                                                                               >  1:746684/1‑150 (MQ=255)
                     aTGAAGTGATCTAAACTGACGAAGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAg                                                                                                                              <  2:67487/150‑1 (MQ=255)
                      tGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAgg                                                                                                                             >  2:243123/1‑150 (MQ=255)
                          gTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCg                                                                                                                         <  2:746684/150‑1 (MQ=255)
                           tGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCgg                                                                                                                        <  2:182738/150‑1 (MQ=255)
                              tCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCt                                                                                                                     >  1:79380/1‑150 (MQ=255)
                                 agaCGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGATCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGc                                                                                                                  <  2:697804/150‑1 (MQ=255)
                                            gaggGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAg                                                                                                       <  1:128501/150‑1 (MQ=255)
                                              gggTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGgc                                                                                                     <  2:218710/150‑1 (MQ=255)
                                                gtgtCGAGGGTGTAGCGGCCGTCAGACATAACGAAGAAGCTGCGGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGgcgc                                                                                                   <  2:1164017/150‑1 (MQ=255)
                                                     gAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCg                                                                                              >  1:732972/1‑150 (MQ=255)
                                                        gCTGTAGAGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATc                                                                                           >  1:164470/1‑150 (MQ=255)
                                                          tGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCt                                                                                         >  2:792178/1‑150 (MQ=255)
                                                          tGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCt                                                                                         <  1:713394/150‑1 (MQ=255)
                                                              gcgcCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCAt                                                                                     <  2:79380/150‑1 (MQ=255)
                                                                gcCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATgg                                                                                   >  1:257681/1‑150 (MQ=255)
                                                                gcCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATgg                                                                                   >  1:59600/1‑150 (MQ=255)
                                                                   aGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGc                                                                                       <  2:737669/143‑1 (MQ=255)
                                                                   aGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGc                                                                                       >  1:737669/1‑143 (MQ=255)
                                                                      cAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAAtgtg                                                                             <  1:243123/150‑1 (MQ=255)
                                                                           aaaatgaaGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCAc                                                                        <  2:1203104/150‑1 (MQ=255)
                                                                                  agaagCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCAt                                                                                     <  1:1115136/130‑1 (MQ=255)
                                                                                  agaagCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCAt                                                                                     >  2:1115136/1‑130 (MQ=255)
                                                                                   gaagCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTAc                                                                >  2:847109/1‑150 (MQ=255)
                                                                                      gCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAAtgtg                                                                             >  2:52106/1‑134 (MQ=255)
                                                                                      gCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAAtgtg                                                                             <  1:52106/134‑1 (MQ=255)
                                                                                          cAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTg                                                         <  2:744218/150‑1 (MQ=255)
                                                                                             gTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTc                                                      <  1:792178/150‑1 (MQ=255)
                                                                                                ccGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTAt                                                   >  2:364641/1‑150 (MQ=255)
                                                                                                  gacgaGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTAttt                                                 <  2:59600/150‑1 (MQ=255)
                                                                                                   acgaGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTg                                                                                        <  1:667960/110‑1 (MQ=255)
                                                                                                   acgaGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTg                                                                                        >  2:667960/1‑110 (MQ=255)
                                                                                                        gCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTTTATGGCaga                                           >  1:51190/1‑150 (MQ=255)
                                                                                                        gCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCaga                                           >  1:310873/1‑150 (MQ=255)
                                                                                                          cccGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGc                                         <  2:1098581/150‑1 (MQ=255)
                                                                                                           ccGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCg                                        >  1:421223/1‑150 (MQ=255)
                                                                                                            cGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCga                                       <  1:847109/150‑1 (MQ=255)
                                                                                                                      agtaagCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGAtgt                             >  2:796275/1‑150 (MQ=255)
                                                                                                                       gtaagCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGAtgtg                            >  1:823309/1‑150 (MQ=255)
                                                                                                                          agCGTAAACCCGAGCGGGGCGACCAAGGTCGCCGCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGCGCCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGc                         <  1:869831/150‑1 (MQ=255)
                                                                                                                                  cccGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGCCCTCCTAAGTACGATTTTGTCCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGc                 <  2:164470/150‑1 (MQ=255)
                                                                                                                                       gCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGACTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGCCttca            >  1:92221/1‑148 (MQ=255)
                                                                                                                                         ggggCGACCTTGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTgg                                               >  2:46375/1‑113 (MQ=255)
                                                                                                                                         ggggCGACCTTGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTgg                                               <  1:46375/113‑1 (MQ=255)
                                                                                                                                          gggCGACCTTGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTg                                                                                        <  2:134306/71‑1 (MQ=37)
                                                                                                                                          gggCGACCTTGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTg                                                                                        >  1:134306/1‑71 (MQ=37)
                                                                                                                                           ggCGACCTTGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGCCTTTAACtt        >  1:6747/1‑150 (MQ=255)
                                                                                                                                                 cAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGCCTTTAACTTCTgcgc  >  1:361420/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCCGTCCGAGGACGAACTCGATGAAGTGATCGAGACGGACGAGGAGGGTGTCGAGGCTGTAGCGCCAGACAATGAAAATGAAGAAGCTGCAGGTGCCGACGAGGCCCCGCAAGAGTAAGTAAGCGTAAACCCGAGCGGGGCGACCAAGGTCGCCCCGTTTGACTGTGTAGGAGCGGGCTTGCCCGCGATGCAGGCGCCACCGATCCTGCATGGAATGTGGCCACCGAATTACGATTTTGTTCTATTTGGCAGAGCGAGAGTGGATGTGAGCAAACGAGCCTTTAACTTCTGCGC  >  NC_002947/1973768‑1974062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑