Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,031,899 T→A 8.7% K130M (AAG→ATG) ‡ kdsA‑II ← 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,031,8990TA 8.7% 140.5 / 3.7 46K130M (AAG→ATG) ‡kdsA‑II2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCCACGATCAGGTTGTCGTAGCCCATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGCCTTCA  >  NC_002947/2031758‑2032046
                                                                                                                                             |                                                                                                                                                   
tCCACGATCAGGTTGTCGTAGCCCATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGtt                                                                                                                                             <  1:361911/150‑1 (MQ=255)
  cACGATCAGGTTGTCGTAGCCCATGCAAGTAAACCTGTCGCAAAGGATCAACTGGTGATTACCGGCTTCCTTGAGCTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGtttt                                                                                                                                           >  2:440595/1‑150 (MQ=255)
           gTTGTCGTAGCCCATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGc                                                                                                                                  >  2:592761/1‑150 (MQ=255)
                  tAGCCCATGCAAGTGCACCTGTCAAAAAGGATCATCTTGTCATTACCCGCGTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACt                                                                                                                           <  2:10892/150‑1 (MQ=255)
                      ccATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAGTTGGGGCTTTTTGATGTTCACCGGCAAGCCGGtgaaca                                                                                                                                         <  2:993968/132‑6 (MQ=255)
                      ccATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAGTTGGGGCTTTTTGATGTTCACCGGCAAGCCGGtgaaca                                                                                                                                         >  1:993968/1‑127 (MQ=255)
                      ccATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGt                                                                                                                       >  2:1050780/1‑150 (MQ=255)
                        aTGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCt                                                                                                                     >  2:573544/1‑150 (MQ=255)
                        aTGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCt                                                                                                                     <  1:1181493/150‑1 (MQ=255)
                         tGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTg                                                                                                                    <  1:573544/150‑1 (MQ=255)
                          gCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGt                                                                                                                   >  1:530466/1‑150 (MQ=255)
                                   ccTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCAAGCCGGtgaacat                                                                                                                                        <  2:964484/120‑7 (MQ=255)
                                   ccTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCAAGCCGGtgaacat                                                                                                                                        >  1:964484/1‑114 (MQ=255)
                                      gTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAg                                                                                                       <  2:737810/150‑1 (MQ=255)
                                       tCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAgg                                                                                                      <  2:1074908/150‑1 (MQ=255)
                                               gATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCgg                                                                                              >  2:819582/1‑150 (MQ=255)
                                                      tGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTgc                                                                                       >  1:842752/1‑150 (MQ=255)
                                                        gTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTg                                                                                                                                          <  1:163105/97‑1 (MQ=255)
                                                        gTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTg                                                                                                                                          >  2:163105/1‑97 (MQ=255)
                                                                ccGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCAGATcga                                                                             >  2:929971/1‑150 (MQ=255)
                                                                 cGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATcgac                                                                            <  2:842752/150‑1 (MQ=255)
                                                                 cGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATcgac                                                                            <  2:530466/150‑1 (MQ=255)
                                                                      ccTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCgg                                                                                              >  2:889550/1‑127 (MQ=255)
                                                                      ccTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCgg                                                                                              <  1:889550/127‑1 (MQ=255)
                                                                        ttGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTcc                                                                     >  1:842747/1‑150 (MQ=255)
                                                                         tGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAg                                                                                                                         >  1:832241/1‑97 (MQ=255)
                                                                         tGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAg                                                                                                                         <  2:832241/97‑1 (MQ=255)
                                                                              ttATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCGCATCGACGACTTCCGCCAcc                                                               >  1:173213/1‑150 (MQ=255)
                                                                              ttATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCAcc                                                               >  2:950878/1‑150 (MQ=255)
                                                                                   cACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGc                                                          >  2:1186044/1‑150 (MQ=255)
                                                                                           tCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGt                                                  >  2:1082734/1‑150 (MQ=255)
                                                                                                      gAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCCAGGCAACAACTAGGTCTGTGTGACCAGCGAGGATTGCCGGCAGCTATATGAAATCGACGACTTCGGCCACCGGGGCCGTTTGGTAGATTTCGTGa                                       <  1:929971/150‑1 (MQ=255)
                                                                                                      gAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGa                                       <  1:1187441/150‑1 (MQ=255)
                                                                                                             tGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGcc                                                                                                >  2:227351/1‑86 (MQ=255)
                                                                                                             tGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGcc                                                                                                <  1:227351/86‑1 (MQ=255)
                                                                                                             tGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTg                                                    >  1:315845/1‑130 (MQ=255)
                                                                                                             tGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTg                                                    <  2:315845/130‑1 (MQ=255)
                                                                                                             tGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCgg                                >  2:432042/1‑150 (MQ=255)
                                                                                                                ggAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGa                             <  1:1082734/150‑1 (MQ=255)
                                                                                                                                    ttCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCt         <  1:592761/150‑1 (MQ=255)
                                                                                                                                      cACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGc       <  2:842747/150‑1 (MQ=255)
                                                                                                                                      cACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGc       <  1:950878/150‑1 (MQ=255)
                                                                                                                                       aCCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGcc      <  1:1050780/150‑1 (MQ=255)
                                                                                                                                           gCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTg                                                    >  1:1020150/1‑100 (MQ=255)
                                                                                                                                           gCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTg                                                    <  2:1020150/100‑1 (MQ=255)
                                                                                                                                           gCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGCCTTCa  >  1:940507/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TCCACGATCAGGTTGTCGTAGCCCATGCAAGTGCCCCTGTCGCAAAGGATCAACTGGTCATTACCCGCTTCCTTGAACTTATGCACGATGTTCTGCATCTGCGAAGGGCTGAGGAATTGGGGCTTTTTGATGTTCACCGGCTTGCCGGTTTTGGCCAAGGCAACAACTAGGTCTGTTTGACGGGCGAGGAATGCCGGCAGCTGCAGCACATCGACGACTTCCGCCACCGGGGCCGTTTGGTAGATTTCGTGAACGTCGGTGATTATCGGCACGCCAAATTCTGCCTTCA  >  NC_002947/2031758‑2032046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑