Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,077,810 A→G 11.1% H164R (CAC→CGC)  PP_1853 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,077,8100AG11.1% 103.8 / 4.0 44H164R (CAC→CGC) PP_1853LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/17);  new base G (3/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

GGAATTCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTGGC  >  NC_002947/2077661‑2077953
                                                                                                                                                     |                                                                                                                                               
ggAATTCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTCCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCa                                                                                                                                                 >  2:1105951/1‑150 (MQ=255)
     tattGCGACGTCACCCAGAGTTTCCGCCGGCTTGGGCTTTCGACAACCGACAGGTCGAGCTGATCGGCCTGGGGTTCTATGCAGCGATTGGCGGTGGTTTCTATCTGCCGTCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCggg                                                                                                                                            <  2:470779/148‑1 (MQ=255)
            ccGATACCCGGCGATTTCCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTtggt                                                                                                                                     <  2:936851/150‑1 (MQ=255)
                   ccAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGgcggcg                                                                                                                              >  2:994958/1‑150 (MQ=255)
                   ccAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGgcggcg                                                                                                                              >  1:740456/1‑150 (MQ=255)
                        gATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGc                                                                                                                         >  2:501081/1‑150 (MQ=255)
                              cccGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCTAGCCACCCCATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCt                                                                                                                   <  2:937701/150‑1 (MQ=255)
                              cccGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCt                                                                                                                   >  2:888768/1‑150 (MQ=255)
                              cccGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCt                                                                                                                   >  1:112296/1‑150 (MQ=255)
                                  gATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAg                                                                                                               >  1:372253/1‑150 (MQ=255)
                                      ggCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCCGCAGCGGc                                                                                                           <  1:731387/150‑1 (MQ=255)
                                         aTTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGTGCGCCggggtca                                                                                                                                        >  2:68654/1‑114 (MQ=255)
                                         aTTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGTGCGCCggggtca                                                                                                                                        <  1:68654/118‑5 (MQ=255)
                                          tttCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGTTGGTTTCGAACTGCCGGCGGGGGTGGTGGCGCGCAAGCTGACATTGGTGCCCCGGGAGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCAcgc                                                                                                       >  1:379013/1‑150 (MQ=255)
                                          tttCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGTAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCAcgc                                                                                                       >  2:619429/1‑150 (MQ=255)
                                          tttCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCAcgc                                                                                                       >  1:911543/1‑150 (MQ=255)
                                                cAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCGGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGaa                                                                                                 >  2:101163/1‑149 (MQ=255)
                                                    cGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGTCCTATCTGCAGCGGCACGCGTCGATagaa                                                                                             <  2:740456/150‑1 (MQ=255)
                                                      ccAGGTTGACATAATCGGCTCGTGCTTCGTTGCCGGGATGGGCCGTGGTTTCTAGCTGGCGCCGGGGGTTGTGGTGCGCGAGCTGACCACGGCGCAGCGGGTGTTGGTGGGGGCGCCGGCCTCTCTGCAGCGGCACGCGTCGATagaaga                                                                                           <  2:379013/150‑1 (MQ=255)
                                                             gACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAg                                                                                    >  1:269595/1‑150 (MQ=255)
                                                                cTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGgcg                                                                                 >  1:164622/1‑150 (MQ=255)
                                                                   aTCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCCGGCGCTg                                                                              <  1:50592/150‑1 (MQ=255)
                                                                              cTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGa                                                                   <  1:714753/150‑1 (MQ=255)
                                                                               atagATACAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGAc                                                                  >  2:75097/4‑150 (MQ=255)
                                                                                       aGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGTAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCt                                                          <  1:619429/150‑1 (MQ=255)
                                                                                        gCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTg                                                         <  1:888768/150‑1 (MQ=255)
                                                                                            ttGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGc                                                     >  1:1039732/1‑150 (MQ=255)
                                                                                                        tCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGa                                         <  1:501081/150‑1 (MQ=255)
                                                                                                            aCTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAg                                                                                    <  2:64494/103‑1 (MQ=255)
                                                                                                            aCTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAg                                                                                    >  1:64494/1‑103 (MQ=255)
                                                                                                              tGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGa                                                                                                  <  2:278880/87‑1 (MQ=255)
                                                                                                              tGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGa                                                                                                  >  1:278880/1‑87 (MQ=255)
                                                                                                                  gccgGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCGTGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGt                               <  2:269595/150‑1 (MQ=255)
                                                                                                                     gggggTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACcgg                                     <  1:763567/141‑1 (MQ=255)
                                                                                                                     gggggTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACcgg                                     >  2:763567/1‑141 (MQ=255)
                                                                                                                          tggtggCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGACGCCGGCCTATCTGCAGCGGCAcgcg                                                                                                      <  1:247802/71‑1 (MQ=255)
                                                                                                                          tggtggCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGACGCCGGCCTATCTGCAGCGGCAcgcg                                                                                                      >  2:247802/1‑71 (MQ=255)
                                                                                                                                      accaacacccGGTGCGCCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCa           >  1:745984/8‑150 (MQ=255)
                                                                                                                                      aGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCa           >  2:743682/1‑150 (MQ=255)
                                                                                                                                       gCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAg          >  2:312166/1‑150 (MQ=255)
                                                                                                                                         aacacccGGTGCGCCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGtt                >  2:1060838/5‑142 (MQ=255)
                                                                                                                                         aacacccGGTGCGCCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGtt                <  1:1060838/138‑1 (MQ=255)
                                                                                                                                          gACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCg       <  2:112296/150‑1 (MQ=255)
                                                                                                                                           aCCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGt      >  2:250510/1‑150 (MQ=255)
                                                                                                                                               cGGCGAACCGGGTGATGGTGGCGGCTCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTGGc  >  2:923367/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GGAATTCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTGGC  >  NC_002947/2077661‑2077953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑