| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,078,282 | T→C | 8.3% | intergenic (+24/+91) | PP_1853 → / ← PP_1854 | LysR family transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,078,282 | 0 | T | C | 8.3% | 132.9 / 3.0 | 48 | intergenic (+24/+91) | PP_1853/PP_1854 | LysR family transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (19/25); new base C (2/2); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
CCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATCGACAATGAACGATACCTGTGCGCCCAGGTGCACCTCGTCA > NC_002947/2078138‑2078424 | ccTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGC < 1:132963‑M1/150‑1 (MQ=255) cTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCG > 1:729467‑M1/1‑150 (MQ=255) tGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGG < 2:857853‑M1/150‑1 (MQ=255) tttCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCC > 1:1093203‑M1/1‑150 (MQ=255) tttCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCC > 2:921996‑M1/1‑150 (MQ=255) tttCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCC > 2:459167‑M1/1‑150 (MQ=255) cGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCGTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCC < 1:567016‑M1/150‑1 (MQ=255) gCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGC < 1:397314‑M1/144‑1 (MQ=255) gCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGC > 2:397314‑M1/1‑144 (MQ=255) aGCACAAGCTGTTGTTGGGCAAGACCCGGGCGTTAATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGTATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAc < 1:1151137/150‑1 (MQ=255) gCACAAGATGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAcc > 2:677194/1‑150 (MQ=255) acaAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCca > 1:263782/1‑150 (MQ=255) caAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCcac > 2:439934/1‑150 (MQ=255) aGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAg < 1:439934/150‑1 (MQ=255) ggCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATcacaa > 1:873909/1‑149 (MQ=255) cGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGt < 1:360720/150‑1 (MQ=255) gTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCTCTGAAGACAGTg < 2:1093203/150‑1 (MQ=255) tttGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTtgt < 1:921996/150‑1 (MQ=255) tgtgGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTgg < 1:459167/150‑1 (MQ=255) tgtgGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTgg < 2:718593/150‑1 (MQ=255) gtggAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGAGGGGTCCTTGTGGGAgg < 1:263917‑M2/150‑3 (MQ=255) ggAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAAGTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgg > 1:939553‑M2/1‑146 (MQ=255) gAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAcc < 1:717621/101‑1 (MQ=255) gAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAcc > 2:717621/1‑101 (MQ=255) cAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggtgcgggcgg < 2:729467‑M1/150‑56 (MQ=255) gTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggcgggc < 1:684870‑M2/150‑11 (MQ=255) cGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggt < 1:550102‑M1/134‑48 (MQ=255) cGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggt > 2:550102‑M1/1‑87 (MQ=255) cGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGAACCGGCCTCTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggtgcgggcgggcccgcga > 2:226489‑M1/1‑87 (MQ=255) gAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGACCTTGTGGGAgcgggcgggcccgcgaa < 1:763904‑M2/150‑18 (MQ=255) gggCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTCTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggtgcgggcgg < 2:125105‑M1/135‑56 (MQ=255) gggCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTCTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggtgcgggcgg > 1:125105‑M1/1‑80 (MQ=255) gCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCgg > 1:997681/1‑150 (MQ=255) cggggcggTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGAACCGGCCTCTTCGCGGGCCCGCCCGCtcccacaaggaccccactgtcttcagggctgtgatatccctgtgggtgcgggcgggcccgcgaaaaggccggcacag < 1:226489‑M1/150‑78 (MQ=255) ttCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATc < 2:763904/150‑1 (MQ=255) gATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGAGATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATCGAc > 2:804047/1‑150 (MQ=255) aTGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCG > 2:334135‑M1/1‑50 (MQ=255) aTGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCG < 1:334135‑M1/50‑1 (MQ=255) ccTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGGTATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggc > 1:847261‑M2/1‑104 (MQ=255) ccTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGGTATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggc < 2:847261‑M2/110‑7 (MQ=255) cTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGAGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATCGACAATGaa < 2:873909/150‑1 (MQ=255) tGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGACCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATCGACAATGAAc < 1:1089200/150‑1 (MQ=255) gCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggcgggcccg > 2:193055‑M2/1‑101 (MQ=255) gCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggcgggcccg < 1:193055‑M2/114‑14 (MQ=255) tttAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGAGGGAGGGCCCGCGCAGAGGCCGGTTCAGACAACAGCGGAAAAGAACGATAACtgt > 1:599404/1‑150 (MQ=255) cTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAgcgggcgggcccgcgaagaggccggttcaggcaataattctctaaagggcctgcagggcatcgaaccgccccg < 1:226489‑M2/150‑74 (MQ=255) tgtgCCGCCCTTTACCCCGGCGAGCCGGATCCTACCGGGAAATCAGGGCCATGCAGACACTGAGGTCATCTCGGCAACGGGCGGTCCCGCGAAGAGGCCGGTTCAGACAACATCGACAAGGAACGATACCTGCGCGAACAGATGCCCCtt < 2:300112/150‑2 (MQ=255) ccGGCCTTTTCGCGGGCCCGCTCGCACCCACAGGGGTATCCCAGCCCTCAAGACAGGGCGGTGCTTGTGGGAGCGGGCGGGCCCGGGAAGAGAGGGGATCCGAAAACTACAGCAAGGAACGAAACGTATGCGCGCAGCAGCTCCTCGTCa > 2:229968/1‑150 (MQ=255) | CCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCCGGTTCAGACAACATCGACAATGAACGATACCTGTGCGCCCAGGTGCACCTCGTCA > NC_002947/2078138‑2078424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |