| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,092,459 | T→C | 13.3% | intergenic (+62/+29) | deaD → / ← ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,459 | 0 | T | C | 13.3% | 107.3 / 6.0 | 44 | intergenic (+62/+29) | deaD/ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (15/23); new base C (3/3); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCTCATCACTGGG > NC_002947/2092315‑2092607 | gCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTcgc < 1:106138/150‑1 (MQ=255) ccTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTcgcg < 2:924938/150‑1 (MQ=255) cTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCgg < 2:1072257/150‑1 (MQ=255) tCGACAAGCTGCTGTCCAAGCTCAAGGACACGCGCGTGTCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCggg < 2:78290/150‑1 (MQ=255) aaGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCTCTTCGCGGGcaagc > 2:20959/1‑146 (MQ=255) gctgctGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGTGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGccc < 1:1010573/150‑1 (MQ=255) tgctgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGAGCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCcgc < 1:20959/150‑1 (MQ=255) gccAAGCTCAAGGGCGCGCGCGTGGCTGGCAAGCGGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAGAGATCCCCGGCTTGGGTCGGGGA‑TTTTTTTTACCTGTGCCGGCCCTTTCGCGGGCTTGCCCGCGAaga < 2:677580/149‑1 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGCCGTTCTCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGCCCCGCCCCCCCCGCCGGCCCGCCCGCCaacggc > 1:677580/1‑146 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaaggg < 1:495904/150‑5 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAgg < 1:1125835/150‑1 (MQ=255) gCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCTGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGccc > 2:202685/1‑150 (MQ=255) aaGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaagggc < 1:1009483/144‑6 (MQ=38) aaGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaagggc > 2:1009483/1‑139 (MQ=38) aaGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTc < 1:2566/149‑1 (MQ=255) aaGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTc > 2:2566/1‑149 (MQ=255) cTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTTGCGTAACGAAAAAATCCCCAAATTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAAc < 1:871938/150‑1 (MQ=255) cTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAAc > 2:729144/1‑150 (MQ=255) tCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAAcc > 2:55318/1‑150 (MQ=255) tggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCTTGGCGAAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGCGCTTGCCCGCGAAGAGGCCCATCAGTCGAACCGCTAGATGt > 2:892358/1‑150 (MQ=255) aaGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATATATGTCCAt > 2:389595/1‑150 (MQ=255) agcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATc < 1:729144/150‑1 (MQ=255) cagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATccc < 1:426392/150‑1 (MQ=255) cTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCa < 1:404412/150‑1 (MQ=255) gCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTcgcg > 1:530686/1‑90 (MQ=255) gCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTcgcg < 2:530686/90‑1 (MQ=255) tACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTaaa > 1:808135/1‑150 (MQ=255) gcgAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTGCATCCCCAGCGCGCCCAGGGTAAAcccc > 1:472909/1‑150 (MQ=255) aTTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTCTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGc < 2:808135/150‑1 (MQ=255) ggCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCg > 2:221403/1‑150 (MQ=255) gAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCt < 2:472909/150‑1 (MQ=255) aaaaaaTCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTc > 2:820578/1‑150 (MQ=255) ggCTTAGGTCGGGGA‑TTTTTTTTACCTGTCCCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAg < 2:53378/150‑1 (MQ=255) tAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCa < 1:55318/150‑1 (MQ=255) ggTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCaaa < 1:221403/150‑1 (MQ=255) ggTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCaaa < 2:872073/150‑1 (MQ=255) tCGGGGA‑‑TTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCATTCAGTCGAAc < 1:1091968/67‑1 (MQ=25) tCGGGGA‑‑TTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCATTCAGTCGAAc > 2:1091968/1‑67 (MQ=25) ggggA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCaaa > 2:245053/1‑146 (MQ=255) ggggA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCaaa < 1:245053/146‑1 (MQ=255) cTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCCGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGc > 2:1165373/1‑150 (MQ=255) cTGTACCGGCCCTGTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCCGGGTAAACCCCTGATCCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGc < 1:1052920/150‑1 (MQ=255) aCCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCtcat < 1:202685/150‑1 (MQ=255) cTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCTCATCACTggg > 1:250132/1‑150 (MQ=255) tttCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCtc > 2:1201720/1‑140 (MQ=255) tttCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCtc < 1:1201720/140‑1 (MQ=255) | GCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGA‑TTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCTCATCACTGGG > NC_002947/2092315‑2092607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |