Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,097,500 C→T 7.3% P135L (CCG→CTG)  gpx → hydroperoxy fatty acid reductase Gpx1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,097,5000CT 7.3% 149.3 / 3.0 55P135L (CCG→CTG) gpxhydroperoxy fatty acid reductase Gpx1
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

TCGGCGTGAGTTTTCCGCTGTTTCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTGGCCAGGTAG  >  NC_002947/2097356‑2097647
                                                                                                                                                |                                                                                                                                                   
tCGGCGTGAGTTTTCCGCTGTTTCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCa                                                                                                                                                >  2:410994/1‑150 (MQ=255)
   gCGTGAGTTTTCCGCTGTTTCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTg                                                                                                                                             <  2:674922/150‑1 (MQ=255)
               cGCTGTTTCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAgc                                                                                                                                 >  1:1025958/1‑150 (MQ=255)
                      tCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGc                                                                                                                          <  2:52938/150‑1 (MQ=255)
                        gCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGccc                                                                                                                        >  1:412424/1‑150 (MQ=255)
                             gtggAGGTAAACGGCCCGGGCGCTCACGCTCTGTTAGTCGAACTCAAGAAACGCGGCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTTGACCCGGCCAGTGGCCAGGTAAAGCGCTATGCCGccagc                                                                                                                   >  2:51167/4‑148 (MQ=255)
                                  ggTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCaa                                                                                                              >  1:106139/1‑150 (MQ=255)
                                      aaCGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGt                                                                                                                                      >  2:1109557/1‑122 (MQ=255)
                                      aaCGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGt                                                                                                                                      <  1:1109557/122‑1 (MQ=255)
                                      aaCGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCca                                                                                                          >  2:593518/1‑150 (MQ=255)
                                       aCGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCcac                                                                                                         <  1:410994/150‑1 (MQ=255)
                                             cGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAgc                                                                                                                                 >  2:223537/1‑120 (MQ=255)
                                             cGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAgc                                                                                                                                 <  1:223537/120‑1 (MQ=255)
                                              gggCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCt                                                                                                  <  2:547480/150‑1 (MQ=255)
                                                 cgcTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGa                                                                                               <  1:303211/150‑1 (MQ=255)
                                                    tCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTCGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGc                                                                                            >  1:1055618/1‑150 (MQ=255)
                                                      aCCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGCTATTTTGGGTAGCCAGCAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCgg                                                                                          <  1:126031/150‑1 (MQ=255)
                                                       cccTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCccaccac                                                                                                                 <  2:853758/126‑1 (MQ=255)
                                                       cccTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCccaccac                                                                                                                 >  1:853758/1‑126 (MQ=255)
                                                        ccTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGAc                                                                                        >  2:59557/1‑150 (MQ=255)
                                                                  tcgaactcgaacAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTTGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGATGCGGACATCGAGCGtt                                                                              <  1:51167/150‑1 (MQ=255)
                                                                  tcgAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGtt                                                                              <  2:1025958/150‑1 (MQ=255)
                                                                       cTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGCACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTc                                                                         <  1:671022/150‑1 (MQ=255)
                                                                        tCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCa                                                                        >  1:1051293/1‑150 (MQ=255)
                                                                                  cgcCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAg                                                              <  2:1055618/150‑1 (MQ=255)
                                                                                    ccccGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAggg                                                            <  2:1051293/150‑1 (MQ=255)
                                                                                     cccGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGTCGGACATCGAGCGTTTGc                                                                           <  2:1077157/134‑1 (MQ=255)
                                                                                     cccGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGTCGGACATCGAGCGTTTGc                                                                           >  1:1077157/1‑134 (MQ=255)
                                                                                             ttttGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGGACTTAATg                                                   <  2:566011/150‑1 (MQ=255)
                                                                                                   gTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGccc                                                                                                   >  1:1096366/1‑96 (MQ=255)
                                                                                                   gTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGccc                                                                                                   <  2:1096366/96‑1 (MQ=255)
                                                                                                         agaagaTCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGc                                       >  2:759402/1‑150 (MQ=255)
                                                                                                          gaagaTCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGAc                                             >  1:839505/1‑143 (MQ=255)
                                                                                                          gaagaTCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGAc                                             <  2:839505/143‑1 (MQ=255)
                                                                                                               tCAAGTGGAACTTCACCAAGTTTCTGGGCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAg                                 <  1:498180/150‑1 (MQ=255)
                                                                                                                 aaGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCAGAGGCCCTGGAGGCGGACATCGAGCATTTCCTAAGCCGCTAAGTGTCACCCAATGGTCGACCAGGGCCAGTAGct                               >  1:269935/1‑150 (MQ=255)
                                                                                                                   gTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCa                                     >  2:545692/1‑142 (MQ=255)
                                                                                                                   gTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCa                                     <  1:545692/142‑1 (MQ=255)
                                                                                                                    tGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGCGCGTTTGCTCAGCCGCTGACGGGCACCCAATAGTCGACCAGCGCCAGTAGGTCtt                            >  2:188637/1‑150 (MQ=255)
                                                                                                                    tGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCtt                            >  2:62687/1‑150 (MQ=255)
                                                                                                                        aCTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGATATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTcgcg                        >  2:1165714/1‑150 (MQ=255)
                                                                                                                               cAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAAt                 >  2:93311/1‑150 (MQ=255)
                                                                                                                                aaGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATg                >  2:929176/1‑150 (MQ=255)
                                                                                                                                  gTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGc              <  1:93311/150‑1 (MQ=255)
                                                                                                                                     tCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTg           <  2:106139/150‑1 (MQ=255)
                                                                                                                                         gTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTGGCCa       >  2:717754/1‑150 (MQ=255)
                                                                                                                                         gTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTGGCCa       <  1:593518/150‑1 (MQ=255)
                                                                                                                                          ttgACCTGGCCACTGGCCGGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGtt                                                                              <  1:509959/76‑1 (MQ=38)
                                                                                                                                          ttgACCTGGCCACTGGCCGGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGtt                                                                              >  2:509959/3‑78 (MQ=38)
                                                                                                                                          tCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCa                                     <  1:119310/119‑1 (MQ=255)
                                                                                                                                          tCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCa                                     >  2:119310/1‑119 (MQ=255)
                                                                                                                                           tgACCTGGCCACTGGCCGGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCt                                                                  >  1:945432/2‑89 (MQ=255)
                                                                                                                                           tgACCTGGCCACTGGCCGGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCt                                                                  <  2:945432/88‑1 (MQ=255)
                                                                                                                                            gACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCgcggc                      <  1:468239/132‑1 (MQ=255)
                                                                                                                                            gACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCgcggc                      >  2:468239/1‑132 (MQ=255)
                                                                                                                                              cccGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTGGCCAGGTAg  <  1:717754/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCGGCGTGAGTTTTCCGCTGTTTCGCAAGGTCGAGGTCAACGGCCCGGGCGCTCACCCTCTGTTCGTCGAACTCAAGCAACGCGCCCCGGGTATTTTGGGTAGCCAGAAGATCAAGTGGAACTTCACCAAGTTTCTGGTCGACCCGGCCAGTGGCCAGGTCAAGCGCTATGCCCCCACCACCAAGCCACAGGCCCTGGAGGCGGACATCGAGCGTTTGCTCAGCCGCTGAAGGGCACCCAATGGTCGACCAGGGCCAGTAGCTCTTCGCGGCGGAATGGCTTGGCCAGGTAG  >  NC_002947/2097356‑2097647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑