Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,115,877 C→A 9.7% L452M (CTG→ATG)  PP_1883 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,115,8770CA 9.7% 118.9 / 2.5 41L452M (CTG→ATG) PP_1883hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/17);  new base A (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GGAGCCTGCGCCGACCGTCAACCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCGGGAATTGAAACTGCATGAGG  >  NC_002947/2115738‑2116024
                                                                                                                                           |                                                                                                                                                   
ggAGCCTGCGCCGACCGTCAACCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGta                                                                                                                                           >  1:226064/1‑150 (MQ=255)
 gAGCCTGCGCCGACCGTCAACCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCTAATCTGGGTAGACGTCTGTGGGTGtac                                                                                                                                          <  1:522335/150‑1 (MQ=255)
                   aaCCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCtgtg                                                                                                                                                 >  1:19776/1‑125 (MQ=255)
                   aaCCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCtgtg                                                                                                                                                 <  2:19776/125‑1 (MQ=255)
                     ccTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCgg                                                                                                                      >  1:945343/1‑150 (MQ=255)
                     ccTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTATGTGGGTGTACTACACCGTCACCCGCGCCgg                                                                                                                      >  1:28191/1‑150 (MQ=255)
                      cTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGTTAGCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCCCGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGc                                                                                                                     >  1:401446/1‑150 (MQ=255)
                              ggAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGcc                                                                                                             >  1:1176331/1‑150 (MQ=255)
                                      aCGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACAt                                                                                                     >  2:904411/1‑150 (MQ=255)
                                      aCGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACAt                                                                                                     <  1:28573/150‑1 (MQ=255)
                                        ggCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCa                                                                                                   <  1:904411/150‑1 (MQ=255)
                                            gcaCGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCtgt                                                                                                                                                  <  1:534360/99‑1 (MQ=255)
                                            gcaCGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCtgt                                                                                                                                                  >  2:534360/1‑99 (MQ=255)
                                             caCGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGaaaa                                                                                              <  1:748226/150‑1 (MQ=255)
                                              aCGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAg                                                                                             >  2:1200250/1‑150 (MQ=255)
                                                 ggggCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTc                                                                                          <  2:226064/150‑1 (MQ=255)
                                                 ggggCAGCCATCGTTCCCCGGCCGCCGTCACGCAGATCGCGCCGGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTc                                                                                          <  2:401446/150‑1 (MQ=255)
                                                 ggggCAGCAATCGTTCCCCGGCCGCCGTCAGGTGACCCACGCCTGCTGTCGGTGCCGCGGTCGGCCATGGCTGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTc                                                                                          <  1:131238/150‑1 (MQ=255)
                                                         cATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGtac                                                                                                                                          >  2:314263/1‑94 (MQ=255)
                                                         cATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGtac                                                                                                                                          <  1:314263/94‑1 (MQ=255)
                                                                    ggCCGCCGTCAGGTTCCACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCCCGCCGGCTAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGAtt                                                                       <  2:110114/150‑1 (MQ=255)
                                                                     gccgccGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTaa                                                                             >  2:511499/1‑143 (MQ=255)
                                                                     gccgccGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTaa                                                                             <  1:511499/143‑1 (MQ=255)
                                                                                gTGACACACGCCTGTTCACGGTGCAGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCa                                                           >  2:675580/1‑150 (MQ=255)
                                                                                         gCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGa                                                  >  2:465270/1‑150 (MQ=255)
                                                                                           cTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGAt                                                                  >  1:217319/1‑132 (MQ=255)
                                                                                           cTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGAt                                                                  <  2:217319/132‑1 (MQ=255)
                                                                                                 aCGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTATGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAg                                          <  2:28191/150‑1 (MQ=255)
                                                                                                  cGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCgg                                                                                 >  2:225456/1‑110 (MQ=255)
                                                                                                  cGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCgg                                                                                 <  1:225456/110‑1 (MQ=255)
                                                                                                   ggTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCTGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGt                                        >  2:872926/1‑150 (MQ=255)
                                                                                                            gTCGGCCATGGCCGCCAATCTGGGTAGACGTATGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTaa                                                                             >  1:663598/1‑104 (MQ=255)
                                                                                                            gTCGGCCATGGCCGCCAATCTGGGTAGACGTATGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTaa                                                                             <  2:663598/104‑1 (MQ=255)
                                                                                                                 ccATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCAATGAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCgaga                          >  1:198249/1‑150 (MQ=255)
                                                                                                                        cgccAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCggg                    <  2:297490/149‑1 (MQ=255)
                                                                                                                        cgccAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCggg                    >  1:297490/1‑149 (MQ=255)
                                                                                                                                 gggTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGc                                                         >  1:813311/1‑103 (MQ=255)
                                                                                                                                 gggTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGc                                                         <  2:813311/103‑1 (MQ=255)
                                                                                                                                     aGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCGGGAATTGaaa            >  2:242748/1‑144 (MQ=255)
                                                                                                                                     aGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCGGGAATTGaaa            <  1:242748/144‑1 (MQ=255)
                                                                                                                                         gTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCGGGAATTGAAACTGCATGAgg  >  1:600927/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GGAGCCTGCGCCGACCGTCAACCTGCATTGGGAAGGGCACGGCAGCACGGGGGCAGCCATCGTTCCCCGGCCGCCGTCAGGTGACACACGCCTGTTCACGGTGCCGCGGTCGGCCATGGCCGCCAATCTGGGTAGACGTCTGTGGGTGTACTACACCGTCACCCGCGCCGGCGAAACGCCACGCACATCAGAAAAGTTCGAGCTGCGGGTAAGCGATTTCGATCAGCGCAGCTACCCGATAATTCAGGTCGAAGGTGCCGAGAACCGGGAATTGAAACTGCATGAGG  >  NC_002947/2115738‑2116024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑