Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,133,379 T→C 6.7% D90G (GAC→GGC)  fimI ← type I pili subunit FimI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,133,3790TC 6.7% 173.2 / 2.6 60D90G (GAC→GGC) fimItype I pili subunit FimI
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base C (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

ATCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGA  >  NC_002947/2133231‑2133523
                                                                                                                                                    |                                                                                                                                                
aTCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTc                                                                                                                                                 >  2:890098/1‑150 (MQ=255)
 tCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCt                                                                                                                                                >  1:395075/1‑150 (MQ=255)
 tCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCAAGAAGGCATTGTCt                                                                                                                                                >  2:972655/1‑150 (MQ=255)
   aaCCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCTGCTTGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTc                                                                                                                                              <  1:579719/150‑1 (MQ=255)
      cGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCt                                                                                                                                           <  1:1195101/150‑1 (MQ=255)
        aGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGGTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGa                                                                                                                                         <  2:700370/150‑1 (MQ=255)
           ttCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGCCTTCGCTg                                                                                                                                          <  2:968951/146‑1 (MQ=255)
           ttCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGCCTTCGCTg                                                                                                                                          >  1:968951/1‑146 (MQ=255)
               aCCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGGTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCa                                                                                                                                  >  1:630184/1‑150 (MQ=255)
                    gTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTg                                                                                                                             <  2:36355/150‑1 (MQ=255)
                     tttGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCACACTGt                                                                                                                            <  2:598815/150‑1 (MQ=255)
                         aTCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtgaa                                                                                                                                         <  1:276082/133‑5 (MQ=255)
                         aTCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtgaa                                                                                                                                         >  2:276082/1‑129 (MQ=255)
                                  ttGGACTTGTGCCTGATCACAATCGAGATGTTTTCTACCGTGCAGGCACTGTTACCAATGCTGAAATAGACGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTAcg                                                                                                               >  2:1084998/1‑150 (MQ=255)
                                        ttGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCACCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTAcgcgtagg                                                                                                         >  2:229425/1‑146 (MQ=255)
                                          gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGtt                                                                                                       >  1:380934/1‑150 (MQ=255)
                                          gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGtt                                                                                                       <  1:890098/150‑1 (MQ=255)
                                            cccTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGa                                                                                                                                <  2:1179948/123‑1 (MQ=255)
                                            cccTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGa                                                                                                                                >  1:1179948/1‑123 (MQ=255)
                                             ccTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTaaa                                                                                                    >  2:1092932/1‑150 (MQ=255)
                                                gATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGa                                                                                                 <  2:650578/150‑1 (MQ=255)
                                                  tCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGAATGTTTTGTCGGGTACGCGGATGTTAAAAGATg                                                                                               <  2:1051216/150‑1 (MQ=255)
                                                           agaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTATTTTGTCGGGTACGCGGATTTAATAAGATGCGCCACGGa                                                                                      >  2:561125/1‑150 (MQ=255)
                                                            gaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGAc                                                                                     >  1:830755/1‑150 (MQ=255)
                                                             aTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACt                                                                                    >  2:928441/1‑150 (MQ=255)
                                                              tGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACtt                                                                                   <  1:1092932/150‑1 (MQ=255)
                                                                  ttCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAAc                                                                               >  1:762297/1‑150 (MQ=255)
                                                                     tgccgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGtt                                                                            >  1:321869/1‑150 (MQ=255)
                                                                       ccgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg                                                                          <  2:380934/150‑1 (MQ=255)
                                                                        cgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGc                                                                         <  1:838460/150‑1 (MQ=255)
                                                                          tgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAg                                                                       <  1:561125/150‑1 (MQ=255)
                                                                             cgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCgg                                                                    >  2:505693/1‑150 (MQ=255)
                                                                             cgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCCCTGATGCTGCAGACTGTTTTGTCGCGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCgg                                                                    >  2:807919/1‑150 (MQ=255)
                                                                                  cTGTTACCAATGCTGAACTACTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGTATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAAt                                                               <  1:776480/150‑1 (MQ=255)
                                                                                      tACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGcc                                                           <  2:830755/150‑1 (MQ=255)
                                                                                       aCCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGccc                                                          <  2:395075/150‑1 (MQ=255)
                                                                                        ccAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGcccc                                                         >  2:640369/1‑150 (MQ=255)
                                                                                         cAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGtt                                                                            <  1:38393/130‑1 (MQ=255)
                                                                                         cAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGtt                                                                            >  2:38393/1‑130 (MQ=255)
                                                                                          aaTGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCggg                                                                   >  2:1065546/1‑138 (MQ=255)
                                                                                          aaTGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCggg                                                                   <  1:1065546/138‑1 (MQ=255)
                                                                                          aaTGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCAt                                                       <  1:640369/150‑1 (MQ=255)
                                                                                                   aTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTc                                              <  2:789144/150‑1 (MQ=255)
                                                                                                    tAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCg                                             <  2:762297/150‑1 (MQ=255)
                                                                                                      gTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGcc                                           <  1:505693/150‑1 (MQ=255)
                                                                                                      gTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGcc                                           <  2:630184/150‑1 (MQ=255)
                                                                                                      gTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCCCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGcc                                           <  1:807919/150‑1 (MQ=255)
                                                                                                       tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgcgc                                                                                            <  1:827317/100‑1 (MQ=255)
                                                                                                       tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgcgc                                                                                            >  2:827317/1‑100 (MQ=255)
                                                                                                          ccgcTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATg                                       >  1:153983/1‑150 (MQ=255)
                                                                                                            gcTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGc                                     >  1:548825/1‑150 (MQ=255)
                                                                                                                    cAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGt                             <  2:548825/150‑1 (MQ=255)
                                                                                                                          cACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGa                       <  2:844683/150‑1 (MQ=255)
                                                                                                                           aCCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGAc                      >  1:636970/1‑150 (MQ=255)
                                                                                                                            ccGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACa                     <  1:1084998/150‑1 (MQ=255)
                                                                                                                              gTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACAtt                   <  2:153983/150‑1 (MQ=255)
                                                                                                                                   aCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAAtt                                                <  1:476841/116‑1 (MQ=255)
                                                                                                                                   aCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAAtt                                                >  2:476841/1‑116 (MQ=255)
                                                                                                                                     ttCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCa            >  2:1024338/1‑150 (MQ=255)
                                                                                                                                               cATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGCTGAGCCACTCACTTGAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGa  <  1:928441/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGA  >  NC_002947/2133231‑2133523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑