Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,136,732 A→T 9.5% L21Q (CTG→CAG)  fadE ← medium‑long chain acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,136,7320AT 9.5% 120.0 / 4.9 42L21Q (CTG→CAG) fadEmedium‑long chain acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base T (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

GTTTGCGTCGCCACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAG  >  NC_002947/2136583‑2136880
                                                                                                                                                     |                                                                                                                                                    
gTTTGCGTCGCCACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCa                                                                                                                                                      <  1:874444/150‑1 (MQ=255)
       tCGCCACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCg                                                                                                                                               >  1:350790/1‑150 (MQ=255)
           cACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGgcgc                                                                                                                                           <  1:1205566/150‑1 (MQ=255)
           cACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGc                                                                                                                                           >  2:802354/1‑150 (MQ=255)
            aCTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCTGGCGccctt                                                                                                                                             <  2:838126/147‑4 (MQ=255)
            aCTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCTGGCGccctt                                                                                                                                             >  1:838126/1‑144 (MQ=255)
            aCTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCg                                                                                                                                          >  1:1184700/1‑150 (MQ=255)
               gCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGa                                                                                                                                       <  1:387130/150‑1 (MQ=255)
                cGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAg                                                                                                                                      <  2:883673/150‑1 (MQ=255)
                cGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAg                                                                                                                                      <  1:181558/150‑1 (MQ=255)
                               ggCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGAt                                                                                                                       <  1:673332/150‑1 (MQ=255)
                                      ttCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGc                                                                                                                >  1:218527/1‑150 (MQ=255)
                                             ccAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCAccacca                                                                                                         <  2:1065445/150‑1 (MQ=255)
                                              cAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATAAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAAGTACGCCGCGCCGATCACCAGCACAaccaa                                                                                                        >  2:293353/1‑150 (MQ=255)
                                                          gATCAGCGCCAGTAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCaa                                                                                            <  1:167945/150‑1 (MQ=255)
                                                           aTCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAAc                                                                                           >  1:457934/1‑150 (MQ=255)
                                                            tCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCacaac                                                                                                 <  2:1014593/143‑1 (MQ=255)
                                                            tCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCacaac                                                                                                 >  1:1014593/1‑143 (MQ=255)
                                                                         ccagccGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGtcctcc                                                                             >  1:184130/1‑150 (MQ=255)
                                                                           agccGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCtt                                                                           <  2:1184700/150‑1 (MQ=255)
                                                                             ccGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTgg                                                                         <  2:184130/150‑1 (MQ=255)
                                                                                ggggCACTACTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAAc                                                                      >  2:38052/1‑150 (MQ=255)
                                                                                         ctgAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCtt                                                             <  1:802354/150‑1 (MQ=255)
                                                                                           gAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGa                                                           >  2:495909/1‑150 (MQ=255)
                                                                                            aaaaTGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACcaca                                                                                                   >  1:1088105/1‑109 (MQ=255)
                                                                                            aaaaTGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACcaca                                                                                                   <  2:1088105/109‑1 (MQ=255)
                                                                                             catgagccATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGc                                                         <  1:67122/144‑1 (MQ=255)
                                                                                             aaaTGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCCACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGc                                                         <  2:350790/150‑1 (MQ=255)
                                                                                                    cATCAACAGCACCTAGGCCGCCACCATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCa                                                  <  2:478302/150‑1 (MQ=255)
                                                                                                     aTCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCAcc                                                                                                                   >  2:92053/1‑84 (MQ=255)
                                                                                                     aTCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCAcc                                                                                                                   <  1:92053/84‑1 (MQ=255)
                                                                                                        aaCAGCACATAGGCCGCCACGATGCCGAGAAGCTCCAAGGGCGCCAGGCGCCAGTGCGTGAGGTACGTCGCGATGAACACCAGCACCAGCAACCACAACAACAACAGAATCGGTCCTCCTAGTAACCAAAGGCTTGACCCATCCcacaca                                              <  2:229273/150‑1 (MQ=255)
                                                                                                                            gATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGa                          >  1:244693/1‑150 (MQ=255)
                                                                                                                                 cGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGc                     >  2:313802/1‑150 (MQ=255)
                                                                                                                                       tCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTc               >  1:244451/1‑150 (MQ=255)
                                                                                                                                           cAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGaa           >  1:1177503/1‑150 (MQ=255)
                                                                                                                                            accggCGCCTGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGa                                                           <  1:1157402/98‑1 (MQ=255)
                                                                                                                                            accggCGCCTGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGa                                                           >  2:1157402/4‑101 (MQ=255)
                                                                                                                                              gggCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGc                                                         >  1:514817/1‑101 (MQ=255)
                                                                                                                                              gggCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGc                                                         <  2:514817/101‑1 (MQ=255)
                                                                                                                                              gggCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAg        <  1:313802/150‑1 (MQ=255)
                                                                                                                                              gggCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAg        >  1:167038/1‑150 (MQ=255)
                                                                                                                                                    cAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAg  >  2:57673/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTTGCGTCGCCACTGCGGGAGCGCAACCAGGGCGATCTTCAGGGCCAGCACCACCCAGATCAGCGCCAGCAGCCAGCCGGGGGCACTGCTGAAAATGCCCATCAACAGCACATAGGCCGCCACGATGCCGAGAATCTGCAAGGGCGCCAGGCGCCGGTGCGTGAGGTACGCCGCGCCGATCACCAGCACCACCAACCACAACAGCAACATAATCGGTCCTCCTTGGAACCAAGGGCTTGAGCCATTCCAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAG  >  NC_002947/2136583‑2136880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑