| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,252,857 | T→A | 6.8% | intergenic (+70/‑49) | leuD → / → PP_1987 | 3‑isopropylmalate dehydratase small subunit/UbiE/COQ5 family methlytransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,252,857 | 0 | T | A | 6.8% | 172.9 / 3.7 | 59 | intergenic (+70/‑49) | leuD/PP_1987 | 3‑isopropylmalate dehydratase small subunit/UbiE/COQ5 family methlytransferase |
| Reads supporting (aligned to +/- strand): ref base T (28/27); new base A (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
AGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGTGAATT > NC_002947/2252714‑2253003 | aGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCcgc > 1:869078/1‑150 (MQ=255) gACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTAAACCcgcgc < 1:139614/150‑2 (MQ=255) aCAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGATCTCGGCGCGGGTTTCCCcccggc < 2:621048/150‑3 (MQ=255) ggCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAaga < 1:806508/149‑1 (MQ=255) aGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTAAACCcgcgccta < 2:1079137/150‑5 (MQ=255) gcgATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCgg > 1:321401/1‑150 (MQ=255) cAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTAGTTTGGTCTGGTATGCCTGAAGTCCGAAGGTGACAGGGCGGTCTTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAg < 1:186451/150‑1 (MQ=37) gCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTGGGCGCGGGTTTACCCGCGAagag > 1:1124182/1‑136 (MQ=255) gCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTGGGCGCGGGTTTACCCGCGAagag < 2:1124182/136‑1 (MQ=255) gCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGcaa < 1:973762/150‑1 (MQ=255) gCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCt > 1:674866/1‑150 (MQ=255) ccAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTa < 1:20631/150‑1 (MQ=255) gcgcCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACga < 1:169473/150‑1 (MQ=255) cGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTACAGGAACGAAACATGCCCAGCACCCAGCACAt < 2:321401/150‑1 (MQ=255) gCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAccagcac < 2:984294/135‑1 (MQ=255) gCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAccagcac > 1:984294/1‑135 (MQ=255) ccTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAccagcacccagca > 2:298944/1‑140 (MQ=255) ccTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAccagcacccagca < 1:298944/140‑1 (MQ=255) gAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTc < 2:869078/150‑1 (MQ=255) ggTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGt > 1:333319/1‑150 (MQ=255) gTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAt > 2:197196/1‑133 (MQ=255) gTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAt < 1:197196/133‑1 (MQ=255) gTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGtt < 2:333319/150‑1 (MQ=255) tGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTc < 2:674866/150‑1 (MQ=255) tGCGGGCCTCCTCGCTCCTACAGACAGCAACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACAAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAg > 1:337911/1‑150 (MQ=255) gCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAt > 1:1006892/1‑125 (MQ=255) gCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAt < 2:1006892/125‑1 (MQ=255) gggCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAg > 1:44503/1‑150 (MQ=255) gCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGc < 1:208390/150‑1 (MQ=255) ctcctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGGCATGACCAGGACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCa > 1:432090/1‑150 (MQ=255) ctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtgt < 2:113391/118‑1 (MQ=255) ctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtgt > 1:113391/1‑118 (MQ=255) ctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAgcg > 2:194361/1‑150 (MQ=255) ctcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATATCAAGCAATGCGACATGACTAGCCCCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAgcg > 1:142485/1‑150 (MQ=38) tcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAcca > 1:244714/1‑98 (MQ=255) tcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGAcca < 2:244714/98‑1 (MQ=255) tcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtg < 2:631485/116‑1 (MQ=255) tcGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGAtg > 1:631485/1‑116 (MQ=255) cGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGc < 1:516201/66‑1 (MQ=255) cGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGc > 2:516201/1‑66 (MQ=255) cTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTaaa < 1:743174/78‑1 (MQ=255) cTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTaaa > 2:743174/1‑78 (MQ=255) tacTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTAc < 2:511228/148‑1 (MQ=255) cTACAGAAAGTTACGCTGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCt > 1:140200/1‑150 (MQ=255) aaaGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGcc > 2:1118104/1‑46 (MQ=255) aaaGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGcc < 1:1118104/46‑1 (MQ=255) tACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCg > 1:861145/1‑150 (MQ=255) gcgGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGt < 1:1136645/148‑1 (MQ=255) gcgGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGt > 2:1136645/1‑148 (MQ=255) gCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGccc > 2:239641/1‑150 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCa > 2:1116313/3‑150 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCa > 1:13271/3‑150 (MQ=255) gcgcGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAg > 2:392569/1‑150 (MQ=255) cgcgGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGt < 1:239641/150‑1 (MQ=255) cgcgGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGt < 2:928133/150‑1 (MQ=255) gcgGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGTg > 1:332515/1‑150 (MQ=255) ggTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCGGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGTGAAt > 2:426483/1‑150 (MQ=255) gTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGTGAAtt > 2:130015/1‑150 (MQ=255) gTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCACAAGGCAGTGAAtt < 2:142485/150‑1 (MQ=255) | AGGACAGCGACGCGATCAAGGCCTTCGAAGCCAAGCACCGCGCCAGCCAGCCTTGGCTGTTCCGTGATGCCTGAAGTTCGTAGGTGACAGTGCGGGCCTCCTCGCTCCTACAGAAAGTTACGCGGTCGCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACAAAAATCTAAAGGAACGAGACATGACCAGCACCCAGCACATCGATGTGGTCCAACGCCAGTTCGGCGAGCAGGCCAGCGCCTACCTCAGCAGCGCCGTGCACGCCCAAGGCAGTGAATT > NC_002947/2252714‑2253003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |