| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 26,844 | A→T | 23.3% | intergenic (+40/+145) | PP_0021 → / ← PP_0022 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 26,844 | 0 | A | T | 23.3% | 86.7 / 26.0 | 43 | intergenic (+40/+145) | PP_0021/PP_0022 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/19); new base T (6/4); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01 | |||||||||||
TTCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCTA > NC_002947/26697‑26991 | ttCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGt > 2:1032575/1‑150 (MQ=255) gAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGt < 1:263595/150‑1 (MQ=255) tCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑CACCACAGTAGTTTa > 2:1030797/1‑150 (MQ=255) ccTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACa > 1:824609/1‑150 (MQ=255) ccTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACa < 2:1084701/150‑1 (MQ=255) cTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAg > 2:1134650/1‑150 (MQ=255) ccGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTg < 1:963906/150‑1 (MQ=255) tGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTa < 2:1194867/150‑1 (MQ=255) tcatcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACaa < 1:624301/150‑1 (MQ=255) catcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAAATGACCGTTTGGTTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTCAACCAGCATGATGCACGGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAAc < 1:822238/150‑1 (MQ=255) aTCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTa < 1:943220/150‑1 (MQ=255) tGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGt < 2:488702/115‑1 (MQ=255) tGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGt > 1:488702/1‑115 (MQ=255) aaGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACt < 1:1073495/150‑1 (MQ=255) aGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTc < 1:874023/150‑1 (MQ=255) gATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGa < 2:77931/131‑1 (MQ=255) gATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGa > 1:77931/1‑131 (MQ=255) ccGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc > 2:1080321/1‑150 (MQ=255) gaACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTgg < 2:793244/130‑1 (MQ=255) gaACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTgg > 1:793244/1‑130 (MQ=255) tGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACAACAATGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCCCGctac > 2:554530/1‑148 (MQ=255) tCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACg > 1:317562/1‑98 (MQ=255) tCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACg < 2:317562/98‑1 (MQ=255) tgctgGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGAtt < 1:1134650/150‑1 (MQ=255) gctgGGGGTGGCTCAGGCCTGAGGAGGCTTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGTCAGCGCGCTCCTCATTGATTc < 2:880444/150‑1 (MQ=38) gctgGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTc < 1:1032575/150‑1 (MQ=255) ggAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGAGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt < 2:857513/150‑1 (MQ=255) aggaggCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac < 2:824609/150‑1 (MQ=255) tAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCgg > 2:842593/1‑150 (MQ=255) cACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTgg < 1:816047/77‑1 (MQ=255) cACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTgg > 2:816047/1‑77 (MQ=255) ttCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAcgc > 2:799078/1‑150 (MQ=38) aCTGTAAACCACTGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctcc < 2:115482/91‑1 (MQ=37) aCTGTAAACCACTGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctcc > 1:115482/1‑91 (MQ=37) aCTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAcgccgc > 1:597448/1‑150 (MQ=38) ctgtaaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTc < 1:1028823/98‑1 (MQ=25) ctgtaaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTc > 2:1028823/6‑103 (MQ=25) tGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCgg > 1:546575/1‑150 (MQ=255) taaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCa < 2:594442/87‑1 (MQ=25) taaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCa > 1:594442/3‑89 (MQ=25) ccACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgtgt < 2:838502/147‑1 (MQ=255) ccACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgtgt > 1:838502/1‑147 (MQ=255) ccACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTcc > 1:211355/1‑150 (MQ=255) cacaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCt > 1:177914/1‑150 (MQ=255) acaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGAGCTCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCta > 1:210482/1‑150 (MQ=255) | TTCGAATCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCTA > NC_002947/26697‑26991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |