Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,305,256	T→C	10.4%	Q448Q (CAA→CAG)	PP_2028 ←	TPR repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,305,256	0	T	C	10.4%	178.2 / 10.3	67	Q448Q (CAA→CAG)	PP_2028	TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base C (3/4);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01

TCGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCGCCTTG  >  NC_002947/2305107‑2305405                                                                                                                                                      |                                                                                                                                                      tCGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGt                                                                                                                                                       >  2:538175/1‑150 (MQ=255)         ggCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGCTTCGCTGACTGCTGTGTCTTGGGTGCTGCTGTCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCaa                                                                                                                                               <  1:749571/150‑2 (MQ=255)           ccTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCa                                                                                                                                             >  1:409465/1‑150 (MQ=255)             tGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAgc                                                                                                                                           <  1:961548/150‑1 (MQ=255)             tGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGGTGCAGCAACTGCTGCaacaac                                                                                                                                           <  1:1070407/150‑6 (MQ=255)              gTTGGTCGTCGCCGGGTGTGCATTGGGCGGAGGTAGTGGCCGAGCTGGTGTTGCAATCGTTTTACCTACCGGGAGTGTCTTCCGCATCGCTGCCGGACGGTTGGACCTCGGCAGTGTCGTCACGCTGTTCCAGCAGTTGCTGCAGCAccg                                                                                                                                          >  2:123158/1‑150 (MQ=255)               ttGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAgcgc                                                                                                                                         >  1:48407/1‑150 (MQ=255)                 ggTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCt                                                                                                                                       <  1:873176/150‑1 (MQ=255)                     gtcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                                   >  1:190367/1‑150 (MQ=255)                              tgtgCCTTGGGCCGGGACGCTGACCGAGATGCTGTTACCTTCTCTTTCGCTGCCGGGCGTGTTTTGGGTATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCACCAGTTGCTGCACCAGCGCCTGGTTGTCCAgcgc                                                                                                                          <  2:735318/150‑1 (MQ=255)                                 gctttGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGCCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGc                                                                                                                       >  2:805709/4‑150 (MQ=255)                                 gCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGc                                                                                                                       <  1:391929/150‑1 (MQ=255)                                  ccTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGcc                                                                                                                      >  2:457340/1‑150 (MQ=255)                                    ttGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTg                                                                                                                    <  1:638497/150‑1 (MQ=255)                                          cGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTc                                                                                                              >  1:155424/1‑150 (MQ=255)                                          cGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCaacaac                                                                                                                                           <  1:969221/121‑6 (MQ=255)                                          cGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCaacaac                                                                                                                                           >  2:969221/1‑116 (MQ=255)                                               tgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGc                                                                                                          >  1:826526/1‑149 (MQ=255)                                               tgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGc                                                                                                          <  2:826526/149‑1 (MQ=255)                                               tgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCCCGACCTGCAGGCCGCGCt                                                                                                         <  2:703857/150‑1 (MQ=255)                                                 ctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCGGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                       <  1:538175/150‑1 (MQ=255)                                                     ccGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctgc                                                                                                                                                 >  2:773115/1‑104 (MQ=255)                                                     ccGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctgc                                                                                                                                                 <  1:773115/104‑1 (MQ=255)                                                       gAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAgcgc                                                                                                                                         <  1:1098028/110‑1 (MQ=255)                                                       gAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAgcgc                                                                                                                                         >  2:1098028/1‑110 (MQ=255)                                                            gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGc                                                                                            <  2:1133701/150‑1 (MQ=255)                                                            gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGc                                                                                            <  2:409465/150‑1 (MQ=255)                                                                  gCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTc                                                                                      <  1:457340/150‑1 (MQ=255)                                                                     ttCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTa                                                                                   >  2:1044986/1‑150 (MQ=255)                                                                      tCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                                  >  2:156901/1‑150 (MQ=255)                                                                         gTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCa                                                                               >  1:1009718/1‑150 (MQ=255)                                                                         gTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCa                                                                               >  2:178579/1‑150 (MQ=255)                                                                                tGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAggg                                                                        <  2:969864/150‑1 (MQ=255)                                                                                  ccGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGggcg                                                                      <  1:29308/150‑1 (MQ=255)                                                                                    tgtcGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGggcggc                                                                    <  2:1472/147‑1 (MQ=255)                                                                                        gtgtCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTcc                                                                >  2:1020443/1‑150 (MQ=255)                                                                                           tcgtGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCa                                                                                                >  1:1204321/4‑115 (MQ=255)                                                                                           tCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCa                                                                                                <  2:1204321/115‑1 (MQ=255)                                                                                           tCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGc                                                             >  1:1066057/1‑150 (MQ=255)                                                                                             ttGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCGGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                                  >  2:534295/1‑127 (MQ=255)                                                                                             ttGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCGGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                                  <  1:534295/127‑1 (MQ=255)                                                                                                 gCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCgg                                                                                                            <  2:924733/97‑1 (MQ=255)                                                                                                 gCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCgg                                                                                                            >  1:924733/1‑97 (MQ=255)                                                                                                 gCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCa                                                       >  1:307731/1‑150 (MQ=255)                                                                                                       cTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCgg                                                 <  1:805709/150‑1 (MQ=255)                                                                                                       cTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACATTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCgg                                                 >  2:346021/1‑150 (MQ=255)                                                                                                        tGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCggg                                                >  1:1083154/1‑150 (MQ=255)                                                                                                        tGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCggg                                                >  1:100177/1‑150 (MQ=255)                                                                                                         gCCGGGCGGTTGTTCCTCGGCCTTGGCTTCAAGTTGTGGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGc                                               <  2:95161/150‑1 (MQ=255)                                                                                                          ccGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTACAGGTCgg                                                                                                            >  1:1177463/1‑88 (MQ=255)                                                                                                          ccGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTACAGGTCgg                                                                                                            <  2:1177463/88‑1 (MQ=255)                                                                                                           cGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCa                                             <  2:190367/150‑1 (MQ=255)                                                                                                                 gttgttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                                   >  2:72608/1‑58 (MQ=255)                                                                                                                 gttgttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                                   <  1:72608/58‑1 (MQ=255)                                                                                                                 gttgttCCTCGGCCTTGGCTTCACATTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCAt                                       <  1:346021/150‑1 (MQ=255)                                                                                                                  ttgttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCAtt                                      <  2:155424/150‑1 (MQ=255)                                                                                                                         tCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGa                               >  2:98556/1‑150 (MQ=255)                                                                                                                          cGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGAGTTCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGAt                              >  1:676079/1‑150 (MQ=255)                                                                                                                               ttGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAg                         >  1:756544/1‑150 (MQ=255)                                                                                                                                         gttgttGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGctgctg               >  1:136843/1‑150 (MQ=255)                                                                                                                                          ctggtgCAGCAACTGCTGCAACAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCtgctgc              >  1:853310/5‑150 (MQ=255)                                                                                                                                           tgttgCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCa             <  1:1044986/150‑1 (MQ=255)                                                                                                                                           tggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCg                                                  <  2:291499/110‑1 (MQ=255)                                                                                                                                           tggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCg                                                  >  1:291499/4‑113 (MQ=255)                                                                                                                                               gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt         >  1:476791/1‑150 (MQ=255)                                                                                                                                                cagcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTc        >  2:1204419/1‑150 (MQ=255)                                                                                                                                                  gcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCGc      >  2:1159122/1‑150 (MQ=255)                                                                                                                                                     gTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCGCCtt   <  2:100177/150‑1 (MQ=255)                                                                                                                                                      ttGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTTTCGCCTTg  <  1:178579/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      TCGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCGCCTTG  >  NC_002947/2305107‑2305405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑