| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,381,445 | T→G | 20.8% | intergenic (+31/+56) | oprF → / ← cobA | porin F/uroporphyrinogen‑III C‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,381,445 | 0 | T | G | 20.8% | 50.8 / 12.1 | 33 | intergenic (+31/+56) | oprF/cobA | porin F/uroporphyrinogen‑III C‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (12/14); new base G (3/4); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
CGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCA > NC_002947/2381301‑2381585 | cGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGcc < 1:360999/150‑1 (MQ=255) gtTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCggg < 1:706465/150‑1 (MQ=255) ttGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGt < 1:251680/150‑1 (MQ=255) cGGTTGGCTCCGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCGAGGCCGGGTTTTTca < 1:650451/150‑2 (MQ=255) tGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGACGAAGTGCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGc < 1:1037008/150‑1 (MQ=255) tACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAACCCCGGCCTCGGCCGGGTTTTTCTTTGCCTgg < 2:741934/150‑1 (MQ=255) cccGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGCACCACCCCGCCTCTGCCGGGTTTTTCTTTGCCTGGGTTTTAACCg < 1:834144/150‑1 (MQ=255) gTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGAAACCATGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTACATTAGAAACCCGGCCTCGGCCGGGTTTTCCTTTGCCTGGGTTTTAACCGGcc > 1:822315/1‑150 (MQ=255) tGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCg > 1:459479/1‑150 (MQ=255) cACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCgg > 2:939754/1‑150 (MQ=255) tCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATcc > 1:576929/1‑150 (MQ=255) tCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGaa > 1:518567/1‑109 (MQ=255) tCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGaa < 2:518567/109‑1 (MQ=255) aaGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCctgct > 2:843681/1‑150 (MQ=255) tCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGGCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTTCGATTGCGATCCGCCCCATAGCCTGCTGCTc > 1:330383/1‑150 (MQ=255) tCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCAAGAAAAACCCGGCCGAGGCCGGGtt > 1:678136/1‑66 (MQ=25) tCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCAAGAAAAACCCGGCCGAGGCCGGGtt < 2:678136/66‑1 (MQ=25) ccAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCct < 1:764533/131‑1 (MQ=255) ccAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCct > 2:764533/1‑131 (MQ=255) tttGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATc > 1:655463/1‑140 (MQ=255) tttGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATc < 2:655463/140‑1 (MQ=255) tttGAAGCTTCATGAAAAACCCGGCCTCGACCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAAGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAAGATTg > 1:1042107/1‑150 (MQ=255) ttGAAGCTTAATGGAACACGTGCCCGCGGCCGGGTTTTCCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGc < 2:822315/150‑1 (MQ=255) tGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGcc < 2:848763/150‑1 (MQ=255) tCATGAAAACCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAAAGGGAACTGGAGCCCGCTCCTGCGGTCGGCTCGTGATTGCGATCATCCCCATAGTCTTCTGCTCCCCAACTACCTCCCCAATCACCAGGATTGCCGGGCTAc < 2:1042107/150‑1 (MQ=255) aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCt < 1:1107943/143‑1 (MQ=255) aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCt > 2:1107943/1‑143 (MQ=255) aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaa < 2:459479/150‑1 (MQ=255) aaaaaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaa < 2:576929/150‑1 (MQ=255) aaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTa < 2:1124784/141‑1 (MQ=255) aaCCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTa > 1:1124784/1‑141 (MQ=255) aaCCCGGCCGAGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCCGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGcaaacca > 2:1159465/1‑150 (MQ=255) aaCCCGGCCGAGACCGGGTTTTTCTTTGCCTGGGTTATAACCGGCCCTGAAGGCCGCTCCTGCGGTCATCCTGCACTTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCCCCTCCCCTATCCCCAGGATTGCCGGGCTACCcaaacca > 2:181064/1‑150 (MQ=255) | CGTGTTGACTCGGTTGGCTACGGCGAAACCCGTCCGGTTGCTGACAACGCCACCGAAGAAGGCCGTGCTGTCAACCGTCGCGTTGAAGCTCAGGTAGAAGCCCAGGCCAAGTAATTGGTTTGAAGCTTCATGAAAAACCCGGCCTCGGCCGGGTTTTTCTTTGCCTGGGTTTTAACCGGCCCTGAAGGCCGCTCCTGCGGTCAGCCTGCGATTGCGATCCGCCCCATAGCCTGCTGCTCCCCAACCACCTCCCCAATCACCAGGATTGCCGGGCTACGCAAACCA > NC_002947/2381301‑2381585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |