| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,425,301 | G→C | 13.8% | R290T (AGG→ACG) | yegS → | lipid kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,425,301 | 0 | G | C | 13.8% | 97.1 / 6.7 | 35 | R290T (AGG→ACG) | yegS | lipid kinase |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base C (3/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGGCTCTGCGGGACCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGAGTTTCCA > NC_002947/2425158‑2425449 | gggggCTCTGCGGGACCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCcggc < 1:1100155/150‑1 (MQ=255) tctGCGGGACGTGTTGGACGGTGACGGCCTGTTCGTTCGGGCCGGGTTGCTATGAGAGGAGGTCAAGAGTTCGCAAGGGTTGGACATCAACGTCGATGGCGCGCCGTTGCAGGCTGCCGGCCTGCGCTTTCAGGCAACGCGGgcggcggt > 2:485393/1‑148 (MQ=255) cGGGACCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAACGCCGCCGGCCTtgcct > 2:905573/1‑148 (MQ=255) cGGGACCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCGCGATGGCGCGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTtgcac < 1:485393/150‑1 (MQ=255) aCCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAACGCCGCCGGCCTtgcctgaaa < 1:905573/150‑7 (MQ=255) ggttcgttcGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGAAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGt < 2:188262/149‑1 (MQ=255) gttcgttcGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGtt < 2:1205488/150‑1 (MQ=255) ttcgGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGAAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCa > 2:697314/1‑150 (MQ=255) ccAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGccggccgg < 1:136259/130‑1 (MQ=255) ccAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGccggccgg > 2:136259/1‑130 (MQ=255) ggTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGAtt > 1:557688/1‑150 (MQ=255) agaTCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTc > 1:48560/1‑150 (MQ=255) agaTCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTc < 2:690325/150‑1 (MQ=255) aTCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGTGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTcgc < 1:562837/150‑1 (MQ=255) aaGAGTTCACAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTa > 2:208419/1‑150 (MQ=255) ggTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCAc < 1:208419/150‑1 (MQ=255) cATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGTCCGGCGGCGTTGCACCTGCACATGCCGGACGGGTCACCGTTGCTCAGATACCCATGCTGATCATTGTTTCGCGTTCTGCGAACAGCACCACGCCg > 2:264074/1‑150 (MQ=255) aTCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTACCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCgg > 1:997169/1‑150 (MQ=255) gcgcgcCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAAt < 1:49937/150‑1 (MQ=255) ccGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCa < 2:48560/150‑1 (MQ=255) gCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGa > 1:114661/1‑150 (MQ=255) aaCCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCAt > 2:656226/1‑145 (MQ=255) aaCCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCAt < 1:656226/145‑1 (MQ=255) aaCCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGAttt > 2:930152/1‑150 (MQ=255) tGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCtga > 2:928345/1‑77 (MQ=255) ggcgcTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCtga < 1:928345/76‑1 (MQ=255) cTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCgg < 1:930152/150‑1 (MQ=255) tttCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGt > 1:291537/1‑150 (MQ=255) tCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGa < 1:697314/150‑1 (MQ=255) tCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGa < 2:557688/150‑1 (MQ=255) cAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGAg < 2:114661/150‑1 (MQ=255) aGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGAGt > 2:1054695/1‑150 (MQ=255) gCAACGCCGCCGGCCTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGa < 1:845346/113‑1 (MQ=255) gCAACGCCGCCGGCCTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGa > 2:845346/1‑113 (MQ=255) aGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGAGTTTCCa < 1:1054695/150‑1 (MQ=255) | GGGGGCTCTGCGGGACCTGTTGGGCGGTGACGGCCTGTTCGTTCGGGCCAGGTTGCCATGGGTCGAGATCAAGAGTTCGCAAGGGTTGGACATCAACCTCGATGGCGAGCCGTTGCAGGCTGCCAACCTGCGCTTTCAGGCAAGGCCGGCGGCGTTGCACCTGCACCTGCCGGCCGGGTCACCGTTGCTCAGTCATCCAGGCTGATGATTTTTTCGCGTACGGCGAACAGCACCAGGCCGGCTACGTCGTGAATCTGCAGGCGCTTCATGATTTGCGAGCGGTGAGTTTCCA > NC_002947/2425158‑2425449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |