| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,607,258 | C→A | 6.4% | S575R (AGC→AGA) | PP_2284 → | tail tubular protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,607,258 | 0 | C | A | 6.4% | 199.2 / 3.2 | 63 | S575R (AGC→AGA) | PP_2284 | tail tubular protein B |
| Reads supporting (aligned to +/- strand): ref base C (26/33); new base A (2/2); total (28/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 | |||||||||||
CACGAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCGGG > NC_002947/2607111‑2607405 | cacGAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCtt < 2:3074/150‑1 (MQ=255)cacGAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCtt > 1:117473/1‑150 (MQ=255) acGAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTg < 2:669537/150‑1 (MQ=255) gAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTggg < 1:284656/150‑1 (MQ=255) aGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCCGACACCGATACCATCCTGAGCGCGGAGTCCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCGTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCa < 1:1088239/150‑1 (MQ=255) gCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCaa > 1:632946/1‑150 (MQ=255) gCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGc < 1:891647/150‑1 (MQ=255) gCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGc < 2:632946/150‑1 (MQ=255) cTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGcc > 1:776216/1‑150 (MQ=255) tCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAAt > 1:715908/1‑144 (MQ=255) tCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAAt < 2:715908/144‑1 (MQ=255) ccTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGt > 1:620007/1‑150 (MQ=255) cTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTa < 1:235906/150‑1 (MQ=255) cTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGc > 1:781440/1‑131 (MQ=255) cTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGc < 2:781440/131‑1 (MQ=255) cTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGa < 2:179699/150‑1 (MQ=255) cTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGa < 2:620007/150‑1 (MQ=255) ttCCCGGACACCTATACCATCATCAGCGCGGAGATCCTGGTGTCAGAAGTCTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGAc < 2:1103352/150‑1 (MQ=255) acCGATGCCATCCCGACCGCGGGGTAGCTGGGCTCCGAAGGGGTTCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCaa > 2:676640/1‑150 (MQ=255) cGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATc < 2:638303/125‑1 (MQ=255) cGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATc > 1:638303/1‑125 (MQ=255) tACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGt > 2:1131925/1‑150 (MQ=255) tCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGa > 1:1132456/1‑150 (MQ=255) tCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGa > 1:1132202/1‑150 (MQ=255) cTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGaaa < 2:117473/150‑1 (MQ=255) cgcgGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCa < 2:536811/140‑1 (MQ=255) cgcgGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCa > 1:536811/1‑140 (MQ=255) ggAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATGTGGTTCACCTGGACCGAAAGCAATACGTCACGCAAGACACCCt > 1:435221/1‑150 (MQ=255) cTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTAc > 2:305584/1‑150 (MQ=255) cTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTAc < 1:305584/150‑1 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGaaa > 1:1100241/1‑129 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGaaa < 2:1100241/129‑1 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGATTGGGCACCAATGAGCCGTATc > 2:1185051/1‑94 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGATTGGGCACCAATGAGCCGTATc > 2:1184978/1‑94 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGATTGGGCACCAATGAGCCGTATc < 1:1185051/94‑1 (MQ=255) tCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGATTGGGCACCAATGAGCCGTATc < 1:1184978/94‑1 (MQ=255) aGTCTTCCTGGTGATCAACCGCGGCAATTGCTTGTACTTCGAAAAGCTCACTGTAACCACGGACAACTGGAGCAACAATGAGCTGTATTTGGTTCCCCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTa < 2:385004/150‑1 (MQ=255) ttCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTAcacc > 1:4720/1‑150 (MQ=255) tCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTAcacca < 1:1131925/150‑1 (MQ=255) cTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTaca > 2:819960/1‑145 (MQ=255) cTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTaca < 1:819960/145‑1 (MQ=255) gcgcCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGt > 2:268621/1‑150 (MQ=255) ccGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTggg < 1:877025/150‑1 (MQ=255) cGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTc > 1:1011965/1‑94 (MQ=255) cGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTc < 2:1011965/94‑1 (MQ=255) cGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACAAATGAGCCGTATCTGGTTCACATGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGc > 1:456394/1‑150 (MQ=255) tGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTAt < 2:824821/150‑1 (MQ=255) gCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATgg < 2:4720/150‑1 (MQ=255) atACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCCGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGa > 2:825974/2‑150 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGa < 2:214742/97‑1 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGa > 1:214742/1‑97 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGc < 2:1091893/111‑1 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGc > 1:1091893/1‑111 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACAccacca > 1:778668/1‑121 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACAccacca < 2:778668/121‑1 (MQ=255) ttCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGc > 1:1015963/1‑150 (MQ=255) gAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACg < 2:1015963/150‑1 (MQ=255) aaaaGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACGt < 1:268621/150‑1 (MQ=255) aaaGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCt < 1:72961/90‑1 (MQ=255) aaaGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCt > 2:72961/1‑90 (MQ=255) gCTCATTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTAAATGGTTCCCGAAGGCCGGGACGTTGACGTGACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACGTACAt < 1:142431/150‑1 (MQ=255) tCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACGTACATgg < 1:825974/150‑1 (MQ=255) aGCTTGGGAACCAATGAGCAGTAGATTGATCAGGTGAGCGTAAAGCAATACTTCACGAAAGACACCCTGAGTTACGCGGATGGCTACCCCACCATCCCGCATTAGTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCggg < 2:435221/150‑1 (MQ=255) | CACGAAGGTGCAAAGCTCCTGGAGCAAGTGGACCTTCCCGGACACCGATACCATCCTGAGCGCGGAGTTCCTGGACTCCGAAGTGTTCCTGGCGATCAACCGCGCCGATGGCTTGTACTTCGAAAAGCTCACTGTAGCCACGGACAGCTTGGGCACCAATGAGCCGTATCTGGTTCACCTGGACCGAAAGCAATACGTCACGAAAGACACCCTGAGTTACGCGGATGGCTACACCACCATCCCGCATTCGTGGGCTATGGATGACGGCACGTACATGGCCGTGACCGCCACCGGG > NC_002947/2607111‑2607405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |