| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,643,810 | T→C | 9.1% | intergenic (+73/‑176) | PP_2313 → / → PP_2314 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,643,810 | 0 | T | C | 9.1% | 119.9 / 3.0 | 45 | intergenic (+73/‑176) | PP_2313/PP_2314 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (22/18); new base C (2/2); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01 | |||||||||||
TGTTGGCCGGCACCGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGACGCCTCCCACCGCTGCCCAGC > NC_002947/2643664‑2643958 | tGTTGGCCGGCACCGTTGCAGTCATGATCGACGGCCCTTGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTc < 2:286192/150‑1 (MQ=255) gTTGGCCGGCACCGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCg > 1:1058391/1‑150 (MQ=255) ggCCGGCACCGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCtt < 2:1058391/150‑1 (MQ=255) cGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCCGGCACATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTgg < 1:1183070/150‑1 (MQ=255) cGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTgg > 1:963499/1‑150 (MQ=255) ggcagtGGCCGCTTCGCGCTCGCCCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGTGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTTTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATa < 2:83955/145‑1 (MQ=255) tctcGCTCGACCTCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑a < 1:788641/150‑1 (MQ=255) ctcgctcgACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑At > 2:158488/1‑150 (MQ=255) gctcgACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTa > 1:1136370/1‑150 (MQ=255) gACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACa > 2:408639/1‑150 (MQ=255) cGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGc > 1:288444/1‑123 (MQ=255) cGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGc < 2:288444/123‑1 (MQ=255) cTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACt > 1:674312/1‑150 (MQ=255) cGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCgg < 1:217631/150‑1 (MQ=255) ccGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGgc > 1:779144/1‑150 (MQ=255) ccGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGgc > 2:245257/1‑150 (MQ=255) gcaaTCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCa > 2:352601/3‑150 (MQ=255) tCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑At < 2:959220/122‑1 (MQ=255) tCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑At > 1:959220/1‑122 (MQ=255) tCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTCGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGaa > 2:1051392/1‑150 (MQ=255) tCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTCGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGaa > 2:1051801/1‑150 (MQ=255) gCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGc < 1:15453/150‑1 (MQ=255) tGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGccc < 1:352601/150‑1 (MQ=255) gtgtgCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCg < 2:1136370/150‑1 (MQ=255) tgCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGc > 1:475149/1‑150 (MQ=255) gCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGATCATCGATCTAGCATACCGCCTGCGCCGCTTTTAAACAGC‑ACAAGTTCGTCCTGGTAAAGCCCCTCCTCCGGGCCGTCCGaa > 2:616256/1‑150 (MQ=255) ccGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCgg > 1:887781/1‑115 (MQ=255) ccGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCgg < 2:887781/115‑1 (MQ=255) gCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGTGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑At > 1:399091/1‑95 (MQ=255) gCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑At < 2:399091/95‑1 (MQ=255) aGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGACAGGTGGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCAAGCGGCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTaa > 1:698963/1‑150 (MQ=255) cgcAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATCACGCTAAAg > 1:1144873/1‑150 (MQ=255) cAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCg > 2:941565/1‑101 (MQ=255) cAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCg < 1:941565/101‑1 (MQ=255) cAATACTCGGCTTGTTCTTGCTTGCGGCTCGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCCTACCGCCTGCGCGG‑TTGTAAACAGCTACGGGCTGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGt < 1:1051801/150‑1 (MQ=255) cAATACTCGGCTTGTTCTTGCTTGCGGCTCGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCACACCGCCTGCGGCT‑GTTTAAACAGCTACGTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGt < 1:1051392/150‑1 (MQ=255) cTCAGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGt < 1:6837/150‑1 (MQ=255) tCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTg > 2:820535/1‑150 (MQ=255) cGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGa < 2:475149/150‑1 (MQ=255) ggCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGt < 1:1057486/147‑1 (MQ=255) ggCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGt > 2:1057486/1‑147 (MQ=255) ttCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATAc < 2:333917/121‑1 (MQ=255) ttCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATAc > 1:333917/1‑121 (MQ=255) ttgcGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGACGCCTCCCACCGCt > 1:35485/1‑150 (MQ=255) gCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGTCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGACGCCTCCCACCGCTGCCCa > 2:471428/1‑150 (MQ=255) ttGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGACGCCTCCCACCGCTGCCCAGc > 1:289249/1‑150 (MQ=255) | TGTTGGCCGGCACCGTTGCAGTCATGATCGAAGGCGCTGGCCGCTTCTCGCTCGACCGCCTGATCGCCCGGTAATCAGCTGCACGCTGTGTGCTACGAGCCGCAGGCAAGCGCAGGCCAATACTCGGCTTGTTCTTGCTTGCGGCTTGTCGCTTGCAACTTGGGGCTCATCGCTCTAGCATACCGCCTGCGCCG‑ATTTAAACAGCTACTTGCGGGGCGCAGGAAAGCCCCACCTCCGGGCCGTCCGAAATACCGCTAAAGCGCTGGTTCGGTGACGCCTCCCACCGCTGCCCAGC > NC_002947/2643664‑2643958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |