Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,726,283 C→A 8.1% S139I (AGT→ATT)  PP_2387 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,726,2830CA 8.1% 182.8 / 3.0 58S139I (AGT→ATT) PP_2387hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base A (2/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTGGTGCGCAGGGCTGCATGCCTTCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACTGTGTGTTC  >  NC_002947/2726153‑2726431
                                                                                                                                  |                                                                                                                                                    
aGGTGGTGCGCAGGGCTGCATGCCTTCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGcc                                                                                                                                   <  2:69375/150‑1 (MQ=255)
                 gCATGCCTTCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGt                                                                                                                  <  2:1159027/150‑1 (MQ=255)
                    tGCCTTCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCt                                                                                                               <  2:856231/150‑1 (MQ=255)
                        ttCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCtggtg                                                                                                           >  2:839923/1‑150 (MQ=255)
                            cTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTc                                                                                                       <  1:242777/150‑1 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCa                                                                                                      >  2:955622/1‑150 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCa                                                                                                      >  2:712211/1‑150 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCAATGAGCACCACCACATGGcc                                                                                                                                   >  1:793439/1‑121 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCAATGAGCACCACCACATGGcc                                                                                                                                   <  2:793439/121‑1 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCAATGAGCACCACCACATGGcc                                                                                                                                   <  2:792463/121‑1 (MQ=255)
                             tAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCAATGAGCACCACCACATGGcc                                                                                                                                   >  1:792463/1‑121 (MQ=255)
                              aaaaCTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAg                                                                                                     <  1:452962/150‑1 (MQ=255)
                               aaaCTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCgg                                                                                                                   <  1:357355/135‑1 (MQ=255)
                               aaaCTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCgg                                                                                                                   >  2:357355/1‑135 (MQ=255)
                               aaaCTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAgg                                                                                                    <  1:955622/150‑1 (MQ=255)
                                   tGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAAca                                                                                                >  1:436118/1‑150 (MQ=255)
                                              gCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTcc                                                                                     >  2:869323/1‑150 (MQ=255)
                                                      ggCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAAcatcat                                                                                            >  2:100532/1‑135 (MQ=255)
                                                      ggCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAAcatcat                                                                                            <  1:100532/135‑1 (MQ=255)
                                                       gCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCg                                                                            >  2:1061807/1‑150 (MQ=255)
                                                         cTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGtt                                                                          <  1:462395/150‑1 (MQ=255)
                                                          ttGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTg                                                                                   >  2:633582/1‑140 (MQ=255)
                                                          ttGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTg                                                                                   <  1:633582/140‑1 (MQ=255)
                                                             gCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGc                                                                      >  1:143597/1‑150 (MQ=255)
                                                               gTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCGGTGGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTCCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGccc                                                                    <  1:318436/150‑1 (MQ=255)
                                                                   tgcGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTg                                                                                                                 >  1:101364/1‑101 (MQ=255)
                                                                   tgcGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTg                                                                                                                 <  2:101364/101‑1 (MQ=255)
                                                                            ttGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAgg                                                       >  1:814286/1‑150 (MQ=255)
                                                                            ttGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAgg                                                       >  1:49532/1‑150 (MQ=255)
                                                                            ttGGTACCTGCGAGGACTTCACACAGGGCTTCGTGAGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGCACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAgg                                                       >  1:546224/1‑150 (MQ=255)
                                                                               gTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCGGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTc                                                    >  1:1084049/1‑150 (MQ=255)
                                                                                tACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCa                                                   >  1:1139666/1‑150 (MQ=255)
                                                                                   cTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTc                                                                                      >  2:840534/1‑112 (MQ=255)
                                                                                   cTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTc                                                                                      <  1:840534/112‑1 (MQ=255)
                                                                                   cTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGcc                                                >  1:656241/1‑150 (MQ=255)
                                                                                      cGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGa                                             <  1:869323/150‑1 (MQ=255)
                                                                                       gAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGtt                                                                                                                              <  1:150712/68‑1 (MQ=255)
                                                                                       gAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGtt                                                                                                                              >  2:150712/1‑68 (MQ=255)
                                                                                                acacaGGGCTTCATGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGTTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGt                                   >  1:669338/1‑150 (MQ=255)
                                                                                                  gcaGGGCTTCGTGGGCCAGGCTGTAGCGGCACATGAGCACCATCTCATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAACTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGa                                 <  2:498407/149‑1 (MQ=255)
                                                                                                  acaGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCa                                                                        <  2:1160746/111‑1 (MQ=255)
                                                                                                  acaGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCa                                                                        >  1:1160746/1‑111 (MQ=255)
                                                                                                  acaGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGcc                                                          <  2:1065615/125‑1 (MQ=255)
                                                                                                  acaGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGcc                                                          >  1:1065615/1‑125 (MQ=255)
                                                                                                     gggCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCCGCGCACTGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCa                              >  1:918829/1‑150 (MQ=255)
                                                                                                         ttCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGa                          <  2:1139666/150‑1 (MQ=255)
                                                                                                                 ccAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCg                  <  2:49532/150‑1 (MQ=255)
                                                                                                                 ccAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCg                  >  2:654191/1‑150 (MQ=255)
                                                                                                                  cAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCa                                               >  1:935519/1‑120 (MQ=255)
                                                                                                                  cAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCa                                               <  2:935519/120‑1 (MQ=255)
                                                                                                                  cAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGa                 <  2:656241/150‑1 (MQ=255)
                                                                                                                           gAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCgg                                                                                                                   >  2:724520/1‑43 (MQ=255)
                                                                                                                           gAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCgg                                                                                                                   <  1:724520/43‑1 (MQ=255)
                                                                                                                           gAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACtgt        <  1:712211/150‑1 (MQ=255)
                                                                                                                              gCCACTGAGCACCACCACATGGCCCATTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACtgtgtg     >  1:1068667/1‑150 (MQ=255)
                                                                                                                              gCCACTGAGCACCACCACATGGCCCATTGGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACtgtgtg     >  1:1068784/1‑150 (MQ=255)
                                                                                                                                 aCTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGTTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACTGTGTGTtc  <  2:669338/150‑1 (MQ=255)
                                                                                                                                 aCTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACTGTGTGTtc  <  1:722505/150‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                    
AGGTGGTGCGCAGGGCTGCATGCCTTCGCTAAAACTGTTGAGAAAGGCCTGGTAGGCCTTGGCGTGCTGCGGGTCGTTGGTACCTGCGAGGACTTCACACAGGGCTTCGTGGGCCAGGCTGTTGAGGCCACTGAGCACCACCACATGGCCGAGTTGGCCAAGGCGGTGCTGGTGGGTCAGGAACATCATCAATTCCTGGGTGGCGTTCAGCCCGCAGCCGGCCAGGCTCAGCCAGACTTCGCCCGTGAGCATCGACAGGTGCGAGGCCACTGTGTGTTC  >  NC_002947/2726153‑2726431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑