Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,745,432 C→G 13.0% V98L (GTA→CTA)  PP_2400 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,745,4320CG13.0% 109.6 / 6.8 38V98L (GTA→CTA) PP_2400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/13);  new base G (2/3);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCA  >  NC_002947/2745289‑2745581
                                                                                                                                               |                                                                                                                                                     
cTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTTTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGt                                                                                                                                                 <  1:1086695/150‑1 (MQ=255)
              gcagcaACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAAc                                                                                                                                   >  1:197884/1‑150 (MQ=255)
                agcaACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCACTGGCCCGGAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAAccc                                                                                                                                 >  1:991129/1‑150 (MQ=255)
                  caACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGAACGAACGGTCACCACCATCAAACCCaa                                                                                                                               <  1:766077/150‑1 (MQ=255)
                        caccaGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCTCTACAACGGTCACCACCATCAAACCCAatgattc                                                                                                                         >  2:442394/1‑148 (MQ=255)
                        caccaGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAc                                                                                                                                                       <  2:485318/120‑1 (MQ=255)
                        caccaGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAc                                                                                                                                                       >  1:485318/1‑120 (MQ=255)
                          ccaGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTcac                                                                                                                                              <  2:1007545/127‑1 (MQ=255)
                          ccaGGGAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTcac                                                                                                                                              >  1:1007545/1‑127 (MQ=255)
                                   tCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTAc                                                                                                              <  2:195307/150‑1 (MQ=255)
                                           aaCTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTc                                                                                                                                                >  1:98637/1‑108 (MQ=255)
                                           aaCTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTc                                                                                                                                                <  2:98637/108‑1 (MQ=255)
                                              ttGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGc                                                                                                   >  1:847629/1‑150 (MQ=255)
                                              ttGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGc                                                                                                   >  2:526432/1‑150 (MQ=255)
                                                   aTTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAcag                                                                                              <  1:967700/150‑1 (MQ=255)
                                                     tGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAcagca                                                                                            <  1:837830/150‑1 (MQ=255)
                                                                tGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGt                                                                                 >  1:466786/1‑150 (MQ=255)
                                                                 ggTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTa                                                                                <  1:643647/150‑1 (MQ=255)
                                                                      gTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAc                                                                                                <  2:976931/129‑1 (MQ=255)
                                                                      gTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAc                                                                                                >  1:976931/1‑129 (MQ=255)
                                                                          tGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCgg                                                                       <  2:847629/150‑1 (MQ=255)
                                                                                      gggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGcc                                                                                                       >  1:664103/1‑106 (MQ=255)
                                                                                      gggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGcc                                                                                                       <  2:664103/106‑1 (MQ=255)
                                                                                       ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                   >  1:1199623/1‑121 (MQ=255)
                                                                                       ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                   <  2:896695/125‑5 (MQ=255)
                                                                                       ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                   <  2:1199623/125‑5 (MQ=255)
                                                                                       ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                   >  1:896695/1‑121 (MQ=255)
                                                                                        gCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGc                                                         >  1:121451/1‑150 (MQ=255)
                                                                                          cAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTg                                                       >  2:715277/1‑150 (MQ=255)
                                                                                            gTTCCGCCAGGCCCGACAAGTGCGCCGGCCAGGGCACTGCCACTATCGCTACAACGGTCACCACGAACAAACCCAATGAAGCCAGCCGGGGACACAGGCCCAGCAACAACAGCAACGGCAGTACCAACTCGGCCCAGATCGAGAGCGGcc                                                     >  2:70428/1‑150 (MQ=255)
                                                                                                    atgCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGc                                             <  2:466786/148‑1 (MQ=255)
                                                                                                                     cGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGtt                            >  1:954415/1‑150 (MQ=255)
                                                                                                                       gCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGa                          >  1:460492/1‑150 (MQ=255)
                                                                                                                          aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAg                                                   >  1:1191166/1‑122 (MQ=255)
                                                                                                                          aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAg                                                   <  2:1191166/122‑1 (MQ=255)
                                                                                                                          aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAgg                       >  1:1037047/1‑150 (MQ=255)
                                                                                                                               aCTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAAc                  >  2:200124/1‑150 (MQ=255)
                                                                                                                                 tGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACgg                >  2:244433/1‑150 (MQ=255)
                                                                                                                                            cTACAACGGTCACCACCATCAAACCCAATGATGCCTGCCGTTTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCcgcg                                       <  1:1113513/116‑1 (MQ=255)
                                                                                                                                            cTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCcgcg                                       >  2:1113513/1‑116 (MQ=255)
                                                                                                                                               cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGcc                                                                     <  1:356808/83‑1 (MQ=255)
                                                                                                                                               cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGcc                                                                     >  2:356808/1‑83 (MQ=255)
                                                                                                                                               cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCa  <  2:460492/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCA  >  NC_002947/2745289‑2745581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑