Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,745,483 G→T 8.0% L81M (CTG→ATG)  PP_2400 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,745,4830GT 8.0% 154.2 / 3.0 50L81M (CTG→ATG) PP_2400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

TTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCGGATTCGA  >  NC_002947/2745335‑2745629
                                                                                                                                                    |                                                                                                                                                  
ttGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGc                                                                                                                                                   >  1:847629/1‑150 (MQ=255)
ttGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGc                                                                                                                                                   >  2:526432/1‑150 (MQ=255)
     aTTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAcag                                                                                                                                              <  1:967700/150‑1 (MQ=255)
       tGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAcagca                                                                                                                                            <  1:837830/150‑1 (MQ=255)
                  tGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGt                                                                                                                                 >  1:466786/1‑150 (MQ=255)
                   ggTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTa                                                                                                                                <  1:643647/150‑1 (MQ=255)
                        gTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAc                                                                                                                                                <  2:976931/129‑1 (MQ=255)
                        gTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAAc                                                                                                                                                >  1:976931/1‑129 (MQ=255)
                            tGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCgg                                                                                                                       <  2:847629/150‑1 (MQ=255)
                                         ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                                                                   <  2:1199623/125‑5 (MQ=255)
                                         ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                                                                   >  1:1199623/1‑121 (MQ=255)
                                         ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                                                                   >  1:896695/1‑121 (MQ=255)
                                         ggCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTAGAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCATCAACGGCAGCAActggt                                                                                                                                   <  2:896695/125‑5 (MQ=255)
                                          gCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGc                                                                                                         >  1:121451/1‑150 (MQ=255)
                                            cAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTg                                                                                                       >  2:715277/1‑150 (MQ=255)
                                              gTTCCGCCAGGCCCGACAAGTGCGCCGGCCAGGGCACTGCCACTATCGCTACAACGGTCACCACGAACAAACCCAATGAAGCCAGCCGGGGACACAGGCCCAGCAACAACAGCAACGGCAGTACCAACTCGGCCCAGATCGAGAGCGGcc                                                                                                     >  2:70428/1‑150 (MQ=255)
                                                      atgCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGc                                                                                             <  2:466786/148‑1 (MQ=255)
                                                                       cGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGtt                                                                            >  1:954415/1‑150 (MQ=255)
                                                                         gCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGa                                                                          >  1:460492/1‑150 (MQ=255)
                                                                            aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAg                                                                                                   >  1:1191166/1‑122 (MQ=255)
                                                                            aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAg                                                                                                   <  2:1191166/122‑1 (MQ=255)
                                                                            aGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAgg                                                                       >  1:1037047/1‑150 (MQ=255)
                                                                                 aCTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAAc                                                                  >  2:200124/1‑150 (MQ=255)
                                                                                   tGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACgg                                                                >  2:244433/1‑150 (MQ=255)
                                                                                              cTACAACGGTCACCACCATCAAACCCAATGATGCCTGCCGTTTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCcgcg                                                                                       <  1:1113513/116‑1 (MQ=255)
                                                                                              cTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCcgcg                                                                                       >  2:1113513/1‑116 (MQ=255)
                                                                                                 cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGcc                                                                                                                     >  2:356808/1‑83 (MQ=255)
                                                                                                 cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGcc                                                                                                                     <  1:356808/83‑1 (MQ=255)
                                                                                                 cAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCa                                                  <  2:460492/150‑1 (MQ=255)
                                                                                                  aaCGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAg                                                 >  1:100492/1‑150 (MQ=255)
                                                                                                            accaTCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAGCGGGAAGCTGGACTGCAGGTCGGCGAAc                                       >  2:92556/1‑150 (MQ=255)
                                                                                                            accaTCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAAc                                       <  1:244433/150‑1 (MQ=255)
                                                                                                                    aCCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTcc                                                                                          >  2:476781/1‑91 (MQ=255)
                                                                                                                    aCCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTcc                                                                                          <  1:476781/91‑1 (MQ=255)
                                                                                                                      ccAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTtgctgc                             >  1:964091/1‑150 (MQ=255)
                                                                                                                      ccAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTtgctgc                             >  1:909097/1‑150 (MQ=255)
                                                                                                                         ataagcccAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGt                          <  1:70428/144‑1 (MQ=255)
                                                                                                                              gCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTTAGATCCGCCGGAAGCAGGATTAAGGGGTACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGACGTTGAAt                     >  1:1047991/1‑150 (MQ=255)
                                                                                                                                cAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAgg                                                                       >  1:713460/1‑98 (MQ=255)
                                                                                                                                cAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAgg                                                                       <  2:713460/98‑1 (MQ=255)
                                                                                                                                     gggTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCa              <  2:117650/150‑1 (MQ=255)
                                                                                                                                     gggTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCa              >  1:117650/1‑150 (MQ=255)
                                                                                                                                       gTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAgg            <  1:715277/150‑1 (MQ=255)
                                                                                                                                        tACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAGCGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGc           <  1:92556/150‑1 (MQ=255)
                                                                                                                                           ccAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAAc                                       >  1:1159203/1‑119 (MQ=255)
                                                                                                                                           ccAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAAc                                       <  2:1159203/119‑1 (MQ=255)
                                                                                                                                           ccAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCgg        >  1:210930/1‑150 (MQ=255)
                                                                                                                                             aGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCGGAt      <  2:100492/150‑1 (MQ=255)
                                                                                                                                              ggCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCGGAtt     <  1:526432/150‑1 (MQ=255)
                                                                                                                                                 ccAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCGGATTCGa  <  2:210930/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTGTAATTGCCGAAGCCCTGGTCGGTAATGGCATAGCCTTGGGCCAGTTCCGCCAGGCCCGACCAGTGCGCCGGCCAGTGCACTGCCACTATCGCTACAACGGTCACCACCATCAAACCCAATGATGCCAGCCGGGTACCCAGGCCCAGCAACAGCAGCAACGGCAGTACCAACTCGGCCCACATCGACAGCTGCCAGTTCAGCTCCGCGGGCAGCAGGTTGAAGGGGAACGGGAAGCTGGACTGCAGGTCGGCGAACCAGTTGCTGCCGTTGAATTTCTCCAGGCCGGATTCGA  >  NC_002947/2745335‑2745629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑