Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,759,083 G→C 10.8% L42V (CTC→GTC)  PP_2412 ← ParA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,759,0830GC10.8% 110.6 / 4.7 37L42V (CTC→GTC) PP_2412ParA family protein
Reads supporting (aligned to +/- strand):  ref base G (17/16);  new base C (2/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GTAGCGGGTTTCGGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTC  >  NC_002947/2758934‑2759225
                                                                                                                                                     |                                                                                                                                              
gtAGCGGGTTCCGGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAAACGGCGACCGGCTGGTCCTCGGGAAGTCGGCCATGCCGGTTGGAATCGTCACGCTATCCAGCTCCGTTTGGTAGAAGGTGGCATTGGCCTGCGggtcgag                                                                                                                                                <  1:574643/150‑1 (MQ=255)
            ggTGATTGCCACGCGGTGCTTCTTACCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCaa                                                                                                                                    >  1:166762/1‑150 (MQ=255)
             gTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTCACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGTGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAg                                                                                                                                   <  1:682584/150‑1 (MQ=255)
                    ccACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGt                                                                                                                            <  1:798689/150‑1 (MQ=255)
                    ccACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATGGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGt                                                                                                                            >  2:645333/1‑150 (MQ=255)
                        gcgGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGcc                                                                                                                        <  1:460574/150‑1 (MQ=255)
                               ttcttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCgg                                                                                                                             <  2:405968/138‑1 (MQ=255)
                               ttcttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCgg                                                                                                                             >  1:405968/1‑138 (MQ=255)
                                  ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                     <  1:671222/127‑1 (MQ=255)
                                  ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                     >  2:671222/1‑127 (MQ=255)
                                  ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGgcggcg                                                                                                              >  2:781609/1‑150 (MQ=255)
                                                   cGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGtt                                                                                             <  2:1140176/150‑1 (MQ=255)
                                                        aCAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTgtcga                                                                                        <  1:645333/150‑5 (MQ=255)
                                                        aCAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAgg                                                                                        <  1:559174/150‑1 (MQ=255)
                                                            ggTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                     <  1:198160/101‑1 (MQ=255)
                                                            ggTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                     >  2:198160/1‑101 (MQ=255)
                                                                                     ccGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTc                                                           <  1:781609/150‑1 (MQ=255)
                                                                                      cGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGc                                                                   >  2:204531/1‑141 (MQ=255)
                                                                                      cGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGc                                                                   <  1:204531/141‑1 (MQ=255)
                                                                                        ggTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCGTTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGa                                                        <  1:701383/150‑1 (MQ=255)
                                                                                         gTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGAt                                                       >  1:1176474/1‑150 (MQ=255)
                                                                                          tGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGAtt                                                      <  2:1176474/150‑1 (MQ=255)
                                                                                               tGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGaaaa                                                 >  2:570158/1‑150 (MQ=255)
                                                                                                    tCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACcaca                                            >  2:83702/1‑150 (MQ=255)
                                                                                                      gTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacg                                          >  1:169722/1‑150 (MQ=255)
                                                                                                       ttGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                         >  1:1018199/1‑150 (MQ=255)
                                                                                                              gCCCGGTGATGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCTGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                  <  1:284341/150‑1 (MQ=255)
                                                                                                              gCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGggtcgag                                                                                                                                                >  2:278004/1‑40 (MQ=255)
                                                                                                              gCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGggtcgag                                                                                                                                                <  1:278004/40‑1 (MQ=255)
                                                                                                              gCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                  <  1:90315/150‑1 (MQ=255)
                                                                                                                        ttcgAAGGTGGCATTGGCCTGCGAGTCGAGGGCGACTAGCAAGAGTGGATGGCCCTCGGCGGCGCTGTCAGCGGTGAGGTTGCAGGCTATGCTGGATTCGCCGACCCCACCTTTCTGGTTTAAAGCAACACGATCCATGAGAGGCTcgtt                        >  2:495444/4‑147 (MQ=255)
                                                                                                                        gTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                         <  2:729017/133‑1 (MQ=255)
                                                                                                                        gTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                         >  1:729017/1‑133 (MQ=255)
                                                                                                                         tAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATcc                       >  1:470559/1‑150 (MQ=255)
                                                                                                                              ggTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGaa                  >  2:429683/1‑150 (MQ=255)
                                                                                                                                   cATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGc                                                                         <  2:854766/90‑1 (MQ=255)
                                                                                                                                   cATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGc                                                                         >  1:854766/1‑90 (MQ=255)
                                                                                                                                      tGGCCTGCGGGTCGAGGTCGACCAGCAAGGTCCGCTCCCGCACCGATTCTCTGGCAGCGGCGAGGTTGCAGGCAAGTCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAAt          <  1:570158/150‑1 (MQ=255)
                                                                                                                                          ttgctggtcgacctcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTcc      >  1:69767/6‑149 (MQ=255)
                                                                                                                                           tGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAg     >  2:733722/1‑150 (MQ=255)
                                                                                                                                              gggTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTc  >  2:392662/1‑150 (MQ=255)
                                                                                                                                                gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCa      >  1:977366/1‑144 (MQ=255)
                                                                                                                                                gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCa      <  2:977366/144‑1 (MQ=255)
                                                                                                                                                 tcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACca                                              <  2:63961/103‑1 (MQ=255)
                                                                                                                                                 tcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACca                                              >  1:63961/1‑103 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GTAGCGGGTTTCGGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTC  >  NC_002947/2758934‑2759225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑