Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,759,091 A→C 9.3% L39R (CTG→CGG)  PP_2412 ← ParA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,759,0910AC 9.3% 117.4 / 2.6 43L39R (CTG→CGG) PP_2412ParA family protein
Reads supporting (aligned to +/- strand):  ref base A (20/19);  new base C (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

GGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTATG  >  NC_002947/2758946‑2759240
                                                                                                                                                 |                                                                                                                                                     
ggTGATTGCCACGCGGTGCTTCTTACCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCaa                                                                                                                                                   >  1:166762/1‑150 (MQ=255)
 gTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTCACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGTGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAg                                                                                                                                                  <  1:682584/150‑1 (MQ=255)
        ccACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGt                                                                                                                                           <  1:798689/150‑1 (MQ=255)
        ccACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATGGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGt                                                                                                                                           >  2:645333/1‑150 (MQ=255)
            gcgGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGcc                                                                                                                                       <  1:460574/150‑1 (MQ=255)
                   ttcttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCgg                                                                                                                                            >  1:405968/1‑138 (MQ=255)
                   ttcttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCgg                                                                                                                                            <  2:405968/138‑1 (MQ=255)
                      ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                                    <  1:671222/127‑1 (MQ=255)
                      ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                                    >  2:671222/1‑127 (MQ=255)
                      ttGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGgcggcg                                                                                                                             >  2:781609/1‑150 (MQ=255)
                                       cGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGtt                                                                                                            <  2:1140176/150‑1 (MQ=255)
                                            aCAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTgtcga                                                                                                       <  1:645333/150‑5 (MQ=255)
                                            aCAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAgg                                                                                                       <  1:559174/150‑1 (MQ=255)
                                                ggTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                                    <  1:198160/101‑1 (MQ=255)
                                                ggTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCTGGTCGACCTCGACCCGCa                                                                                                                                                    >  2:198160/1‑101 (MQ=255)
                                                                         ccGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTc                                                                          <  1:781609/150‑1 (MQ=255)
                                                                          cGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGc                                                                                  <  1:204531/141‑1 (MQ=255)
                                                                          cGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGc                                                                                  >  2:204531/1‑141 (MQ=255)
                                                                            ggTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCGTTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGa                                                                       <  1:701383/150‑1 (MQ=255)
                                                                             gTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGAt                                                                      >  1:1176474/1‑150 (MQ=255)
                                                                              tGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGAtt                                                                     <  2:1176474/150‑1 (MQ=255)
                                                                                   tGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGaaaa                                                                >  2:570158/1‑150 (MQ=255)
                                                                                        tCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACcaca                                                           >  2:83702/1‑150 (MQ=255)
                                                                                          gTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacg                                                         >  1:169722/1‑150 (MQ=255)
                                                                                           ttGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                                        >  1:1018199/1‑150 (MQ=255)
                                                                                                  gCCCGGTGATGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCTGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                                 <  1:284341/150‑1 (MQ=255)
                                                                                                  gCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATga                                                 <  1:90315/150‑1 (MQ=255)
                                                                                                            ttcgAAGGTGGCATTGGCCTGCGAGTCGAGGGCGACTAGCAAGAGTGGATGGCCCTCGGCGGCGCTGTCAGCGGTGAGGTTGCAGGCTATGCTGGATTCGCCGACCCCACCTTTCTGGTTTAAAGCAACACGATCCATGAGAGGCTcgtt                                       >  2:495444/4‑147 (MQ=255)
                                                                                                            gTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                                        >  1:729017/1‑133 (MQ=255)
                                                                                                            gTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACacga                                                        <  2:729017/133‑1 (MQ=255)
                                                                                                             tAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATcc                                      >  1:470559/1‑150 (MQ=255)
                                                                                                                  ggTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGaa                                 >  2:429683/1‑150 (MQ=255)
                                                                                                                       cATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGc                                                                                        <  2:854766/90‑1 (MQ=255)
                                                                                                                       cATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGc                                                                                        >  1:854766/1‑90 (MQ=255)
                                                                                                                          tGGCCTGCGGGTCGAGGTCGACCAGCAAGGTCCGCTCCCGCACCGATTCTCTGGCAGCGGCGAGGTTGCAGGCAAGTCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAAt                         <  1:570158/150‑1 (MQ=255)
                                                                                                                              ttgctggtcgacctcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTcc                     >  1:69767/6‑149 (MQ=255)
                                                                                                                               tGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAg                    >  2:733722/1‑150 (MQ=255)
                                                                                                                                  gggTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTc                 >  2:392662/1‑150 (MQ=255)
                                                                                                                                    gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCa                     >  1:977366/1‑144 (MQ=255)
                                                                                                                                    gtcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCa                     <  2:977366/144‑1 (MQ=255)
                                                                                                                                     tcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACca                                                             <  2:63961/103‑1 (MQ=255)
                                                                                                                                     tcgaggtcgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACca                                                             >  1:63961/1‑103 (MQ=255)
                                                                                                                                            cgaCCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATc       <  1:961877/150‑1 (MQ=255)
                                                                                                                                                 aGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTATg  >  1:457438/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGTGATTGCCACGCGGTGCTTCTTGCCGGCGGCGGTAACCGGCGACAAGGTCTGGCGGAAGAAGTCGGCGATGCCGGGTGGAATGGTGTCGTTGACCAGCCCGGTGAGGTAGAAGGTGGCATTGGCCTGCGGGTCGAGGTCGACCAGCAAGGTTCGGTAGCCCTCGGCGGCGCTGGCAGCGGCGAGGTTGCAGGCGATGCTGGATTTGCCGACGCCACCTTTCTGATTGAAAACCACACGACGCATGAGACGCTCATCCATGAAGTAAAAATGTCAGGTCGGGGTCAATCCTATG  >  NC_002947/2758946‑2759240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑