Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,775,309 T→G 10.8% A82A (GCA→GCC)  PP_5521 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,775,3090TG10.8% 84.2 / 4.3 37A82A (GCA→GCC) PP_5521hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/19);  new base G (2/2);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

ATACGCACCACCTGGCCCTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTCCCAGCCGTGCGCACGGCCCTGCGCGACGTA  >  NC_002947/2775160‑2775449
                                                                                                                                                     |                                                                                                                                            
aTACGCACCACCTGGCCCTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAAt                                                                                                                                              <  2:620611/150‑1 (MQ=255)
    gCACCACCTGGCCCTTGTCGTAAAGCAAGCCAATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACAGGGCGGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGGGGGGTTGGCCAGGCCTTTCGGAATGCCt                                                                                                                                          >  2:60136/1‑150 (MQ=255)
      accaccTGGCCCTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTgg                                                                                                                                        <  2:133611/150‑1 (MQ=255)
       ccaccTGGCCCTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGgc                                                                                                                                       >  1:711724/1‑150 (MQ=255)
                 cTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTtc                                                                                                                             >  2:137641/1‑150 (MQ=255)
                        tAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACg                                                                                                                      >  1:268781/1‑150 (MQ=255)
                               tGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCATTCCGCAAGGCCTgg                                                                                                                                        >  2:509681/1‑125 (MQ=255)
                               tGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCATTCCGCAAGGCCTgg                                                                                                                                        <  1:509681/125‑1 (MQ=255)
                               tGCCGATGCGGGCCAGCAGGCTGCAATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCGTGGCGCTGCTGTTCGTCGACGCCAGGag                                                                                                               >  1:352103/1‑149 (MQ=255)
                                  cGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGt                                                                                                            <  1:138252/150‑1 (MQ=255)
                                         ggCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGctgc                                                                                                     <  2:266322/150‑1 (MQ=255)
                                            cagcagGCTGCCATCTTCCAGAAGCAGTACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGctgcctg                                                                                                  >  1:1068483/1‑150 (MQ=255)
                                             agcagGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGctgcctgc                                                                                                 >  1:312064/1‑150 (MQ=255)
                                                   cTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCt                                                                                           <  2:394761/150‑1 (MQ=255)
                                                   cTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCt                                                                                           <  2:268781/150‑1 (MQ=255)
                                                       cATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACa                                                                                       <  2:881414/150‑1 (MQ=255)
                                                         tCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGAc                                                                                        >  1:207625/1‑147 (MQ=255)
                                                         tCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGAc                                                                                        <  2:207625/147‑1 (MQ=255)
                                                          cTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGcc                                                                                    <  2:78637/150‑1 (MQ=255)
                                                           ttCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCa                                                                                   >  1:395555/1‑150 (MQ=255)
                                                                     agGACATGGCTGCTGCCTTGGCCGTGGTCTTCGAGCACCGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCAAGGCGTCGAGc                                                                         <  1:854913/150‑1 (MQ=255)
                                                                         cATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGgt                                                                     <  1:137641/150‑1 (MQ=255)
                                                                          aTGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCCAGCAGgtg                                                                    <  2:352103/150‑1 (MQ=255)
                                                                                     cTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCgg                                                         <  2:711724/150‑1 (MQ=255)
                                                                                          ccGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTc                                                                             <  2:397437/125‑1 (MQ=255)
                                                                                          ccGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTc                                                                             >  1:397437/1‑125 (MQ=255)
                                                                                           cGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCt                                                   >  1:456533/1‑150 (MQ=255)
                                                                                               gTCTTAGAACAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGAAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCgg                                               <  1:149437/150‑1 (MQ=255)
                                                                                                       gCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTAGCgg                                                         >  1:485867/1‑132 (MQ=255)
                                                                                                       gCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTAGCgg                                                         <  2:485867/132‑1 (MQ=255)
                                                                                                       gCAACGGTATCAAGGGGTCGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGa                                       <  2:1113412/150‑1 (MQ=255)
                                                                                                         aaCGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAgg                                     <  2:1068483/150‑1 (MQ=255)
                                                                                                              tATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTc                                >  1:577655/1‑150 (MQ=255)
                                                                                                                                gggTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGc                                                                                >  2:253095/1‑84 (MQ=255)
                                                                                                                                gggTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGc                                                                                <  1:253095/84‑1 (MQ=255)
                                                                                                                                     gCCAGGCATTCCGCAAGGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTCCCAGCCGTGCGCACGGCCCTgc          <  2:402806/149‑1 (MQ=255)
                                                                                                                                     gCCAGGCATTCCGCAAGGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTCCCAGCCGTGCGCACGGCCCTgc          >  1:402806/1‑149 (MQ=255)
                                                                                                                                            cTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTCCCAGCCGTGCGCACGGCCCTGCGCGACGTa  <  2:788667/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
ATACGCACCACCTGGCCCTTGTCGTAAGGCATGCCGATGCGGGCCAGCAGGCTGCCATCTTCCAGCAGCAGGACATGGCTGATGCCTTGGCCGTGGTCTTCGAGCAACGGTATCAAGGGGTGGTGTGCGGGTGGCCAGGCCTTGCGGAATGCCTGGCGCTGCTGTTCGTCGACGCCAGGACGGTTGGCTGCCTGCAGGGCTGACAGCCAGGCGTCGAGCAGGTGTTCGGTGGCGGGTGCCTGCGGCTCTTCGAGGTTGTCCCAGCCGTGCGCACGGCCCTGCGCGACGTA  >  NC_002947/2775160‑2775449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑