| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,841,978 | T→A | 12.4% | intergenic (‑114/+138) | yqhD ← / ← PP_2493 | NAD(P)‑dependent alcohol dehydrogenase/RadC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,841,978 | 0 | T | A | 12.4% | 92.0 / 5.6 | 34 | intergenic (‑114/+138) | yqhD/PP_2493 | NAD(P)‑dependent alcohol dehydrogenase/RadC family protein |
| Reads supporting (aligned to +/- strand): ref base T (15/14); new base A (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
TTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTA > NC_002947/2841831‑2842126 | ttCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgcttg < 1:1131742/150‑1 (MQ=255) tGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTa > 1:609079/1‑150 (MQ=255) ggggTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTaa > 1:583896/1‑150 (MQ=255) ggTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAg < 1:878306/150‑1 (MQ=255) ttGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCAAGCTTCTaaccaa < 2:384318/150‑5 (MQ=255) tgGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAAc > 2:602683/1‑150 (MQ=255) aCTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCt > 2:1019567/1‑150 (MQ=255) tAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTaaa < 1:1120199/102‑1 (MQ=255) tAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTaaa > 2:1120199/1‑102 (MQ=255) aCCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgctt < 2:986214/90‑1 (MQ=255) aCCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgctt > 1:986214/1‑90 (MQ=255) aCCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGACGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGATCCAAAg > 2:175681/1‑150 (MQ=255) tCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGtt < 2:714969/150‑1 (MQ=255) ggTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAagcagc < 2:702887/150‑1 (MQ=255) ggTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAagcagc < 2:583896/150‑1 (MQ=255) gAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTa > 1:845438/1‑150 (MQ=255) tttttATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCt > 1:1008852/1‑150 (MQ=255) tgaTTGCTGAAGCTCGTCGGCGCGCATTGGGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGGAACCAAGTTGGTGTGGCATTCGTCTGCACTGCTTCTCGCTAGCCAAAAGCGTTGCCGCCAAGCAGCGTAAGCTccc < 2:18408/148‑2 (MQ=255) cAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGAt > 2:825960/1‑150 (MQ=255) aaGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTc < 1:825960/150‑1 (MQ=255) tcgGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAagcagc < 2:214040/124‑1 (MQ=255) tcgGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAagcagc > 1:214040/1‑124 (MQ=255) gcgcgcATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCcgc < 2:331529/132‑1 (MQ=255) gcgcgcATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCcgc > 1:331529/1‑132 (MQ=255) cgcATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCt < 1:602683/150‑1 (MQ=255) cATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAg > 2:183802/1‑150 (MQ=255) aTTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTACAGGCTGGTGAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTGGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAgg > 1:936759/1‑150 (MQ=255) aTTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGgttg > 2:468961/1‑142 (MQ=255) aTTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGgttg < 1:468961/142‑1 (MQ=255) gcACCAAATAGCTGAAGATTGGTTAGATCGCTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACtg > 1:937938/1‑150 (MQ=255) cACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACtga < 2:609079/150‑1 (MQ=255) cAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACtgatga < 2:1114243/150‑1 (MQ=255) aTAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAg > 2:87692/1‑150 (MQ=255) ttgcctttAGAAGCAAGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCggg > 2:606683/7‑150 (MQ=255) aTTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGa < 2:845438/150‑1 (MQ=255) ctttAGAAGCAAGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAg < 1:302968/110‑1 (MQ=255) ctttAGAAGCAAGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAg > 2:302968/3‑112 (MQ=255) cttgcttCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTa > 1:864434/1‑150 (MQ=255) ttgcttCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGa > 2:821228/1‑55 (MQ=255) ttgcttCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGa < 1:821228/55‑1 (MQ=255) | TTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTA > NC_002947/2841831‑2842126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |