| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 210,699 | T→A | 7.4% | L5402Q (CTG→CAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 210,699 | 0 | T | A | 7.4% | 144.6 / 4.1 | 54 | L5402Q (CTG→CAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (28/22); new base A (2/2); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
GTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGT > NC_002947/210551‑210847 | gTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTg > 1:968605/1‑150 (MQ=35) tCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTgg > 2:932273/1‑150 (MQ=35) aCGTCGGTCACCGACGAGCGAGGGACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTg < 2:517110/150‑1 (MQ=21) cGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCTACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGa < 1:879365/150‑1 (MQ=25) cgacgaGCCAGGTTCCACAGGCACCGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGcaa < 2:968605/150‑1 (MQ=255) cgacgaGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGcaa > 2:1116623/1‑150 (MQ=35) gaGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGACGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAAt < 2:588703/150‑1 (MQ=25) cAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCGACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGccc < 2:612043/150‑1 (MQ=38) cAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCa < 1:273523/150‑1 (MQ=33) aGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGG‑TCACGACCAGGTCGTGag < 2:817345/122‑2 (MQ=255) aGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGG‑TCACGACCAGGTCGTGag > 1:817345/1‑121 (MQ=255) gAATGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGcc > 2:504941/1‑150 (MQ=17) gAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGcc > 1:1111846/1‑150 (MQ=32) gAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGa > 2:440052/1‑115 (MQ=17) gAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGa < 1:440052/115‑1 (MQ=17) cGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCga < 1:1099419/150‑1 (MQ=32) gACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCCCTGTTCACGTCGACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCgag > 1:1161698/1‑150 (MQ=17) tGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCa < 1:1164334/150‑1 (MQ=32) tCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGCGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAgcg > 1:315068/1‑150 (MQ=17) tCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCCGCGAGACCAgcg > 2:796910/1‑150 (MQ=17) aGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCCAGGCCGGCGAGACCAgcgcgc > 2:1076769/1‑150 (MQ=17) aCCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTac > 1:50238/1‑150 (MQ=35) cATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTacac > 2:393219/1‑150 (MQ=35) cATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTacac > 1:795883/1‑150 (MQ=35) aCCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCAc > 1:1093675/1‑150 (MQ=35) gACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAggg < 1:894787/150‑1 (MQ=32) ttCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGa > 1:278098/1‑150 (MQ=32) ttCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGa > 2:1137669/1‑150 (MQ=32) ttCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGa < 1:1137669/150‑1 (MQ=32) cGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTa < 1:1076769/150‑1 (MQ=32) tCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATg < 1:932273/150‑1 (MQ=32) aaaCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGTGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCt > 2:802029/1‑150 (MQ=17) aaCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACGCCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTg > 1:299518/1‑150 (MQ=17) aaCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTg > 2:244339/1‑150 (MQ=32) aCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTgg > 2:454757/1‑150 (MQ=32) gACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCa < 1:393219/150‑1 (MQ=32) aCCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCa > 2:398446/1‑149 (MQ=32) aCCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCa < 1:398446/149‑1 (MQ=32) cTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGAt > 2:19993/1‑150 (MQ=32) tGTTCACGTGAACCAGGCGCTGGCCCACGAACTGGTCGTGCCGCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCt < 2:315068/150‑1 (MQ=11) ttCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACAAGGTTGGCCAGATCGGCCTgg > 1:509597/1‑150 (MQ=255) cGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCAt < 1:244339/150‑1 (MQ=35) cGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCAt < 1:19993/150‑1 (MQ=35) gTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATc > 1:418621/1‑150 (MQ=35) gTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATc < 1:504941/150‑1 (MQ=35) gCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGt < 2:1111846/150‑1 (MQ=35) ggCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGa > 1:869422/1‑150 (MQ=35) gCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGTGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGAc < 1:802029/150‑1 (MQ=25) tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTacac > 2:280496/1‑75 (MQ=35) tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTacac < 1:280496/75‑1 (MQ=35) tCACGACCTGGTCGTGACCCTGAGCAACAAGGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGc < 1:306187/150‑1 (MQ=25) cACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGcc > 1:6778/1‑150 (MQ=35) cTGGTCGTGACCCTGCGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGt > 2:1168667/1‑150 (MQ=37) cTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGt > 1:457012/1‑150 (MQ=37) | GTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGT > NC_002947/210551‑210847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |