Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	233,832	A→T	10.8%	intergenic (+20/+43)	PP_0180 → / ← PP_0181	cytochrome c family protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	233,832	0	A	T	10.8%	105.0 / 5.1	37	intergenic (+20/+43)	PP_0180/PP_0181	cytochrome c family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/16);  new base T (2/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

GACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCC  >  NC_002947/233690‑233981                                                                                                                                               |                                                                                                                                                      gACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGAc                                                                                                                                                >  2:135298/1‑150 (MQ=255) gACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGAc                                                                                                                                                >  2:848845/1‑150 (MQ=255)         ggggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAg                                                                                                                                        <  2:534387/150‑1 (MQ=255)         ggggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAg                                                                                                                                        >  1:534387/1‑150 (MQ=255)           ggATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGt                                                                                                                                      <  1:311846/150‑1 (MQ=255)                     cGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGGGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCtt                                                                                                                            >  1:946453/1‑150 (MQ=255)                     cGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCtt                                                                                                                            <  1:767749/150‑1 (MQ=255)                      gATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTg                                                                                                                           <  2:1078564/150‑1 (MQ=255)                              cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtctc                                                                                                                                   >  1:1092744/1‑134 (MQ=255)                              cTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGtctc                                                                                                                                   <  2:1092744/134‑1 (MQ=255)                                   tGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATg                                                                                                              <  2:452104/150‑1 (MQ=255)                                                      cTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGgcgc                                                                                           >  1:192533/1‑150 (MQ=255)                                                              ccATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGaa                                                                                   >  1:947088/1‑150 (MQ=255)                                                              ccATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGaa                                                                                   <  2:192533/150‑1 (MQ=255)                                                                      ttCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGt                                                                           >  1:952833/1‑150 (MQ=255)                                                                       tCCTTTACCGCCGCTCGCGGCGGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTa                                                                          <  2:69576/150‑1 (MQ=255)                                                                        ccTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAg                                                                         >  1:255453/1‑150 (MQ=255)                                                                          tttACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGaa                                                                                   >  2:485502/1‑138 (MQ=255)                                                                          tttACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGaa                                                                                   <  1:485502/138‑1 (MQ=255)                                                                                       gTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAAt                                                                                  >  1:912917/1‑126 (MQ=255)                                                                                       gTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAAt                                                                                  <  2:912917/126‑1 (MQ=255)                                                                                            tggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTg                                                                   <  2:916785/136‑1 (MQ=255)                                                                                            tggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTg                                                                   >  1:916785/1‑136 (MQ=255)                                                                                             ggcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACCCTTTAAACCGc                                                    <  2:952833/150‑1 (MQ=255)                                                                                              gcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCa                                                                                                          >  2:524127/1‑95 (MQ=255)                                                                                              gcCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCa                                                                                                          <  1:524127/95‑1 (MQ=255)                                                                                                        gcAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCa                                         >  2:427129/1‑150 (MQ=255)                                                                                                          aGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCGAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGGTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAgg                                       >  2:472808/1‑150 (MQ=255)                                                                                                             gctgcGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTg                                    <  1:135298/150‑1 (MQ=255)                                                                                                                       actgactgGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgc                          <  1:427129/150‑1 (MQ=255)                                                                                                                         tgactgGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAgccg                        >  1:842678/1‑150 (MQ=255)                                                                                                                                  cttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCt                                                                             >  2:1185775/3‑88 (MQ=37)                                                                                                                                  cttGCGCGGTCCCTGTGGGACCGCGCAGGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCt                                                                             <  1:1185775/86‑1 (MQ=37)                                                                                                                                   ttGCGCGGTCCCTGTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTc                                                 >  2:1134900/2‑115 (MQ=255)                                                                                                                                   ttGCGCGGTCCCTGTGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTc                                                 <  1:1134900/114‑1 (MQ=255)                                                                                                                                           tCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATc          >  1:908315/1‑146 (MQ=255)                                                                                                                                           tCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATc          <  2:908315/146‑1 (MQ=255)                                                                                                                                              cacaGGGACCGAGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGc   <  1:848845/150‑1 (MQ=255)                                                                                                                                               acaGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGcc  >  2:923422/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                      GACTGGCTGGGGATTCATGCCGATGCCTACTCGCTGTCGGCGCAAGCGGTGGCCTTGCTGGCCATCGTGTTCCTTTACGGCCGCTCGTGGCTGGCCGAAAAGCGCAGTGCTGCGGCCAACTGACTGGAGCCTGCGCGGTCCCACAGGGACCGCGCAAGGTCTCAGATCTTGCGCAAGCCTGATGCTCAGGCTGGCTGTGGCGCCTTGAGAATGTTCTGTAGCAATTGGACACTTTCAACGGCGTCATGACCCAGGCTGGTCAGGTAGCCGCCATCCGGCTGATCGGTCAGCC  >  NC_002947/233690‑233981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑