| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,099,491 | C→G | 11.3% | A76G (GCC→GGC) | PP_2709 → | long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,099,491 | 0 | C | G | 11.3% | 101.2 / 3.9 | 35 | A76G (GCC→GGC) | PP_2709 | long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (12/19); new base G (2/2); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GCGCAGCGTTACACCTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGCCGAACAGCTCATTGCGCTTGGCTTTGCCCGGCAGGCGCCAGCTG > NC_002947/3099349‑3099640 | gcgcAGCGTTACACCTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAc‑gc < 2:68686/150‑1 (MQ=255) gcAGCGTTACACCTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑gcgc > 1:1131217/1‑150 (MQ=255) acCTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACa < 1:536821/150‑1 (MQ=255) cTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGt > 2:240340/1‑150 (MQ=255) cGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTgccg > 2:65610/1‑150 (MQ=255) aaCGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAgc > 2:45799/1‑150 (MQ=255) aaCGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGC‑GTTCGGCGAACAGCGccg < 1:1065350/124‑2 (MQ=255) aaCGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGC‑GTTCGGCGAACAGCGccg > 2:1065350/1‑123 (MQ=255) aaGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGa > 1:1068090/1‑150 (MQ=255) tCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGC‑GTTCGGCGAACAGCGccg > 1:588198/1‑116 (MQ=255) tCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGC‑GTTCGGCGAACAGCGccg < 2:588198/117‑2 (MQ=255) cgcaagcAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTgca < 2:1200984/145‑1 (MQ=255) gcAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCaaa < 1:240340/150‑1 (MQ=255) aGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGc < 2:862566/150‑1 (MQ=255) gCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACTCAGTTCCAGCACTGCTTGCAGCAAAGCg < 1:65610/150‑1 (MQ=255) accaccGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTCTTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAgg > 1:125146/1‑149 (MQ=255) caccGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAg < 1:471292/147‑1 (MQ=255) caccGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAg > 2:471292/1‑147 (MQ=255) ccGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGAcg < 2:934743/150‑1 (MQ=255) cGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGAcgg > 1:127276/1‑150 (MQ=255) ggAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGAcggcg < 2:269865/150‑1 (MQ=255) ccTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACTCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCg < 2:1068090/150‑1 (MQ=255) cTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCgt > 2:544565/1‑150 (MQ=255) cTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCACCACTGCTTGCAGCAAATCGGAGGGACGGCGTTCgt > 2:315957/1‑150 (MQ=255) cGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGa < 2:452995/150‑1 (MQ=255) tggtgCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAgg < 2:1131217/150‑1 (MQ=255) ggATAACGGGCCCGAAGCGCTATTCCGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTg < 1:444553/150‑1 (MQ=255) aaCGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGcc < 1:222664/150‑1 (MQ=255) ccGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTggg < 1:690368/141‑1 (MQ=255) ccGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTggg > 2:690368/1‑141 (MQ=255) ccGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGCCGAACAGc < 2:975743/150‑1 (MQ=255) cggggATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCTGTCGTGTGTGAGGCGCAGTGGGCCGAACAGCTCATTGCGCTTgg < 2:525688/148‑1 (MQ=255) gATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGCCGAACAGCTCATTGCGCTTGGCttt < 1:315957/150‑1 (MQ=255) tGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGcc < 2:291531/113‑1 (MQ=255) tGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGcc > 1:291531/1‑113 (MQ=255) ccGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGCCGAACAGCTCATTGCGCTTGGCTTTGCCCGGCAGGCGCCAGCTg > 1:1023137/1‑150 (MQ=255) | GCGCAGCGTTACACCTACCGGCAACTGCTCAACGAAGTCGAATCCCGTGCGAGCTGCTTGCGCAGCCAACCACCGGGAACCTTCGCCGTGGTGCTGGATAACGGGCCCGAAGCGCTATTCTGGGATTTGGCGGCGCTGTTCGCCGAAC‑GCGCCAGTGTGACAGTGCCGCCGTTTTTCAGCGCGACCCAGTTCCAGCACTGCTTGCAGCAAAGCGGAGTGACGGCGGTCGTGTGTGAGGCGCAGTGGGCCGAACAGCTCATTGCGCTTGGCTTTGCCCGGCAGGCGCCAGCTG > NC_002947/3099349‑3099640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |