Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,146,657 G→A 14.6% intergenic (+50/+16) PP_2761 → / ← sfnA ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,146,6570GA14.6% 90.0 / 9.1 42intergenic (+50/+16)PP_2761/sfnAribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein
Reads supporting (aligned to +/- strand):  ref base G (15/19);  new base A (4/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

TCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTGCGCCAG  >  NC_002947/3146510‑3146805
                                                                                                                                                   |                                                                                                                                                    
tCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGcc                                                                                                                                                    <  1:509035/150‑1 (MQ=255)
    cTGGAATTTCTTCTGGGAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAg                                                                                                                                                <  1:1092578/150‑1 (MQ=255)
     tGGAATTTCTTCTGGCATCAGTTGGCCAGCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAgg                                                                                                                                               <  2:373373/150‑1 (MQ=255)
          tttCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCAGCGCCTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGTGACGTCAGTGTCGTGACGAGGGCTGGGGGTTTCCCCCGGGCATg                                                                                                                                          >  1:186416/1‑150 (MQ=255)
            tcttctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCt                                                                                                                                        >  2:847076/1‑150 (MQ=255)
              ttctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATCGTTCTGGCGTTCCGGGCGGCACGCCAGTGTCGTTACGAGGCCTGGGGTAGTCCCCGGGCCATGCttt                                                                                                                                      <  2:318093/150‑1 (MQ=255)
                ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAgg                                                                                                                                               >  1:864240/1‑139 (MQ=255)
                ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAgg                                                                                                                                               <  2:864240/139‑1 (MQ=255)
                 tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                                   <  2:1066140/150‑1 (MQ=255)
                 tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                                   <  1:701084/150‑1 (MQ=255)
                   gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTGGTCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGa                                                                                                                                 >  1:633318/1‑150 (MQ=255)
                          gTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCGGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTg                                                                                                                          >  1:227927/1‑150 (MQ=255)
                              ccACCGGCGTTCGGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTGGGGGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCATGACGAGGCTTGGGGGATGCCCCAGGCCCGGCTTTCGGTACTCCTCGGCAc                                                                                                                      >  2:211490/1‑150 (MQ=255)
                               cACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCAcc                                                                                                                     <  1:728656/150‑1 (MQ=255)
                                    tcGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCAc                                                                                                                <  2:186416/149‑1 (MQ=255)
                                    gCGTTCTTATTCTCTCGGTTCTGCTGTTCAGTTGTGCCAGGCGGCGCCTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCTTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCAc                                                                                                                <  1:215629/150‑1 (MQ=255)
                                       ttCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                                   >  1:946651/1‑128 (MQ=255)
                                       ttCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                                   <  2:946651/128‑1 (MQ=255)
                                           gATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTg                                                                                                         >  1:341047/1‑150 (MQ=255)
                                            aTTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGc                                                                                                        <  2:591627/150‑1 (MQ=255)
                                               ctctcGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcat                                                                                                     <  2:227927/150‑1 (MQ=255)
                                                                      tGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCAtt                                                                              <  1:847076/150‑1 (MQ=255)
                                                                        ccAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGTGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGc                                                                                                        <  1:688587/122‑1 (MQ=255)
                                                                        ccAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGc                                                                                                        >  2:688587/1‑122 (MQ=255)
                                                                                    tCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGc                                                                >  2:990616/1‑150 (MQ=255)
                                                                                      ggCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTGGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCg                                                              >  2:410820/1‑150 (MQ=255)
                                                                                               tAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAg                                                                                               <  2:1070284/108‑1 (MQ=255)
                                                                                               tAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAg                                                                                               >  1:1070284/1‑108 (MQ=255)
                                                                                                  ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCAcc                                                                                                                     >  2:103832/1‑83 (MQ=255)
                                                                                                  ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCAcc                                                                                                                     <  1:103832/83‑1 (MQ=255)
                                                                                                       gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCAGGc                                                                                                                                              <  1:80886/53‑1 (MQ=37)
                                                                                                       gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCAGGc                                                                                                                                              >  2:80886/1‑53 (MQ=37)
                                                                                                        cGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGc                                                                                                                             <  2:1144731/69‑1 (MQ=39)
                                                                                                        cGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGc                                                                                                                             >  1:1144731/1‑69 (MQ=39)
                                                                                                                 cGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCggg                                   <  2:995813/150‑1 (MQ=255)
                                                                                                                    aaCGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTtg                                >  1:302127/1‑150 (MQ=255)
                                                                                                                                gTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCa                    <  1:594528/150‑1 (MQ=255)
                                                                                                                                   gcatggCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTa                 >  2:770458/5‑150 (MQ=255)
                                                                                                                                   gcatggCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTa                 >  1:730779/5‑150 (MQ=255)
                                                                                                                                         ccTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCg           >  2:292080/1‑150 (MQ=255)
                                                                                                                                          cTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGt          <  1:770458/150‑1 (MQ=255)
                                                                                                                                                  tGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTGCGCCAg  >  2:773665/1‑150 (MQ=255)
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TCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTGCGCCAG  >  NC_002947/3146510‑3146805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑