Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,195,808 A→T 9.7% L101Q (CTG→CAG)  dhaT ← 1,3‑propanediol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,195,8080AT 9.7% 153.2 / 5.3 51L101Q (CTG→CAG) dhaT1,3‑propanediol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base T (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGGGATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGCCTGCTGCC  >  NC_002947/3195673‑3195957
                                                                                                                                       |                                                                                                                                                     
gTCGGGATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATa                                                                                                                                         >  1:211229/1‑150 (MQ=255)
 tCGGGATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATAc                                                                                                                                        <  2:211229/150‑1 (MQ=255)
  cGGGATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGACGTCGTGTGGGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCTGCTGGCCGtgggtggc                                                                                                                                       <  2:286481/150‑8 (MQ=255)
   gggATGGCGACGATGGGCGGGATGGCATGGGTAAGCTTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATAccc                                                                                                                                      >  1:400290/1‑150 (MQ=255)
   gggATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATAccc                                                                                                                                      <  2:587941/150‑1 (MQ=255)
       tGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGc                                                                                                                                  >  1:992795/1‑150 (MQ=255)
           gacgaTGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCg                                                                                                                              >  2:195662/1‑150 (MQ=255)
                 gggCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCAGATTTGCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAgcg                                                                                                                        >  1:146085/1‑150 (MQ=255)
                   gCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCt                                                                                                                      <  2:976837/150‑1 (MQ=255)
                      ggATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTggg                                                                                                                   >  1:250273/1‑150 (MQ=255)
                            cATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGgtgt                                                                                                             <  2:43389/150‑1 (MQ=255)
                              tGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTgg                                                                                                           <  1:188756/150‑1 (MQ=255)
                                   atacGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGAt                                                                                                      <  1:709979/147‑1 (MQ=255)
                                   aaGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGAt                                                                                                      <  2:532068/150‑1 (MQ=255)
                                          aaCGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGGCGTCGATACCCGTGCCCCGATAGCGCTGGGCGgtgt                                                                                                             >  1:73510/1‑136 (MQ=255)
                                          aaCGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGgtgt                                                                                                             <  2:73510/136‑1 (MQ=255)
                                             gACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGtgtg                                                                                                            <  1:1175622/134‑1 (MQ=255)
                                             gACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGtgtg                                                                                                            >  2:1175622/1‑134 (MQ=255)
                                             gACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTg                                                                                            <  1:195662/150‑1 (MQ=255)
                                               cccTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCcagca                                                                                                                                                    <  1:1137776/92‑1 (MQ=255)
                                               cccTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCcagca                                                                                                                                                    >  2:1137776/1‑92 (MQ=255)
                                                ccTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTgcgc                                                                                         >  2:33908/1‑150 (MQ=255)
                                                ccTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTgcgc                                                                                         <  2:250273/150‑1 (MQ=255)
                                                     gTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGtt                                                                                    <  1:33908/150‑1 (MQ=255)
                                                      tAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGa                                                                                                                             <  1:1069213/108‑1 (MQ=255)
                                                      tAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTg                                                                                   >  2:607020/1‑150 (MQ=255)
                                                      tAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATACATCGTGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGa                                                                                                                             >  2:1069213/1‑108 (MQ=255)
                                                       aGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTgg                                                                                  >  2:1170440/1‑150 (MQ=255)
                                                          ccTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGAt                                                                                                                            <  2:69550/105‑1 (MQ=255)
                                                          ccTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGAt                                                                                                                            >  1:69550/1‑105 (MQ=255)
                                                           cTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCTGCTGGCCGtgggt                                                                                                                                          >  2:340577/1‑86 (MQ=255)
                                                           cTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCTGCTGGCCGtgggt                                                                                                                                          <  1:340577/90‑5 (MQ=255)
                                                             cGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGa                                                                            <  1:315570/150‑1 (MQ=255)
                                                               aTACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATg                                                                          >  1:782930/1‑150 (MQ=255)
                                                                tACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGt                                                                         >  1:836865/1‑150 (MQ=255)
                                                                  cGGCCGTCGTGGGTGAGCGGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGGCAGGAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTcc                                                                       <  1:209081/150‑1 (MQ=255)
                                                                    gCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGAGGGATGGTCTTGATGTCCGc                                                                     >  2:936488/1‑150 (MQ=255)
                                                                                 agcagcAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAg                                                        <  1:607020/150‑1 (MQ=255)
                                                                                  gcagcaGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAgg                                                       >  1:42981/1‑150 (MQ=255)
                                                                                        ggCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATAc                                                 >  2:433379/1‑150 (MQ=255)
                                                                                            gATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGAt                                                                           <  2:104872/120‑1 (MQ=255)
                                                                                            gATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGAt                                                                           >  1:104872/1‑120 (MQ=255)
                                                                                                           atccatcGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGc                              <  1:433379/150‑1 (MQ=255)
                                                                                                               atcGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAg                          >  2:509090/1‑150 (MQ=255)
                                                                                                                      tGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGt                   >  1:1119441/1‑150 (MQ=255)
                                                                                                                              tcgacGGCCTGCTGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGa                         >  1:76391/4‑136 (MQ=255)
                                                                                                                              tcgacGGCCTGCTGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGa                         <  2:76391/133‑1 (MQ=255)
                                                                                                                              cccacGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAgg           <  2:782930/150‑1 (MQ=255)
                                                                                                                               ccacGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGc          >  2:368446/1‑150 (MQ=255)
                                                                                                                               ccacGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGc          >  2:939313/1‑150 (MQ=255)
                                                                                                                                cacGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGcc         >  1:723742/1‑150 (MQ=255)
                                                                                                                                cacGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGcc         >  1:54750/1‑150 (MQ=255)
                                                                                                                                     ccAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGCctgctg    <  2:992795/150‑1 (MQ=255)
                                                                                                                                       agcagGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGCCTGCTGcc  <  2:723742/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                     
GTCGGGATGGCGACGATGGGCGGGATGGCATGGGTAAGCGTGAACGACCCTTCGTAGTCCTCGATACGGCCGTCGTGGGTGAGCAGCAGGCTGATGGCCTTGGCCGCATCCATCGCGCTGCCGCCACCCACGGCCAGCAGGCCGTCGATACCCGTGCCCCGATAGCGCTGGGCGGTGTGGTTGATGTCGTCACTGCGCGGGTTGGCCTTGATGTCCGCCACTTCCTCGTAGGCGATACCGGCCTTGTCGAGGCTGGCGTAGACGCTGTCCAGCAGGCCTGCTGCC  >  NC_002947/3195673‑3195957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑