| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,212,321 | A→T | 7.4% | *189R (TGA→AGA) ‡ | PP_2816 ← | transcriptional regulator NfxB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,212,321 | 0 | A | T | 7.4% | 166.0 / 4.2 | 54 | *189R (TGA→AGA) ‡ | PP_2816 | transcriptional regulator NfxB |
| Reads supporting (aligned to +/- strand): ref base A (27/23); new base T (2/2); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGC > NC_002947/3212173‑3212456 | tATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCAGCTCGCAGAGGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCaa < 1:1051321/150‑1 (MQ=255) gCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGgc > 1:671377/1‑150 (MQ=255) gACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCcgcg > 1:904090/1‑150 (MQ=255) cATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTgg > 1:859375/1‑150 (MQ=255) aTCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGc > 2:1158837/1‑150 (MQ=255) aGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTg > 2:1104302/1‑150 (MQ=255) aTCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCCCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTa < 2:164882/150‑1 (MQ=255) ttGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCtctc > 2:44040/1‑150 (MQ=255) ttGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTACGCGGGCGCCCTATTGAGCGCCcgcgcagg < 2:942500/126‑5 (MQ=38) ttGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTACGCGGGCGCCCTATTGAGCGCCcgcgcagg > 1:942500/1‑122 (MQ=38) gATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGtt < 1:172336/150‑1 (MQ=255) tCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGc < 1:1158837/150‑1 (MQ=255) ggAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGcaca < 1:120161/150‑1 (MQ=255) cACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGGACCGTTGCACActct > 2:840615/1‑150 (MQ=255) cgccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACActctct < 1:1104302/150‑1 (MQ=255) cgccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACActctct > 2:806055/1‑150 (MQ=255) gccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCtt > 1:623592/1‑150 (MQ=255) ccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAcgcg > 1:66428/1‑122 (MQ=255) ccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAcgcg < 2:66428/122‑1 (MQ=255) ccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTg < 2:623592/150‑1 (MQ=255) ccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTg < 1:44040/150‑1 (MQ=255) cgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGt < 2:662948/99‑1 (MQ=255) cgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGt > 1:662948/1‑99 (MQ=255) gATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGccc < 1:674102/90‑1 (MQ=255) gATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGccc > 2:674102/1‑90 (MQ=255) aaTAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcg < 2:403404/150‑1 (MQ=255) gCGTTCATTCCCTTCCTTTGACGCAATAGGCCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgggcg > 1:19267/1‑150 (MQ=255) tCATTCCCTTCCTTTTGCGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa < 1:924564/150‑1 (MQ=255) cccTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTc > 2:722082/1‑143 (MQ=255) cccTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTc < 1:722082/143‑1 (MQ=255) cccTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatg > 2:909367/1‑150 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa > 1:148996/1‑144 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa < 2:148996/144‑1 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa < 2:662234/144‑1 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa > 1:662234/1‑144 (MQ=255) ccttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgc > 1:567028/1‑150 (MQ=255) cttccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgcc > 2:753597/1‑150 (MQ=255) tccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgccat < 2:567028/150‑1 (MQ=255) ccttTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGAccatgccatg < 2:592115/150‑1 (MQ=255) tttGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCa > 1:61358/1‑150 (MQ=255) ttGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCaa < 1:806055/150‑1 (MQ=255) gCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCg > 1:1197980/1‑150 (MQ=255) aTAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcg > 2:350424/1‑121 (MQ=255) aTAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcg < 1:350424/121‑1 (MQ=255) gTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGaa > 1:170489/1‑150 (MQ=255) tCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAAc < 2:904090/150‑1 (MQ=255) tCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACACCGATACGAAc > 1:471547/1‑150 (MQ=255) ttCACGGCTGTTGCCCTGCGCGGGAGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACAt > 1:587033/1‑150 (MQ=255) cGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCg < 1:753597/150‑1 (MQ=255) gCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGt < 2:671377/150‑1 (MQ=255) ccTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgggc > 1:767655/1‑103 (MQ=255) ccTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcgggc < 2:767655/103‑1 (MQ=255) cTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAAcgc > 1:369893/1‑150 (MQ=255) tGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgcgcgc > 2:705207/1‑85 (MQ=255) tGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGgcgcgc < 1:705207/85‑1 (MQ=255) | TATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGC > NC_002947/3212173‑3212456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |