Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,252,540 T→C 16.3% intergenic (+27/+216) ureG → / ← PP_2851 urease accessory protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,252,5400TC16.3% 93.5 / 11.6 43intergenic (+27/+216)ureG/PP_2851urease accessory protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/14);  new base C (4/3);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

GCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC  >  NC_002947/3252392‑3252685
                                                                                                                                                    |                                                                                                                                                 
gcgACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTc                                                                                                                                                  >  1:1128569/1‑150 (MQ=255)
    cacCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGca                                                                                                                                              <  2:552795/150‑1 (MQ=255)
    cacCCAGCGCAAGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGca                                                                                                                                              >  2:50306/1‑150 (MQ=255)
       ccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACag                                                                                                                                           >  1:326440/1‑150 (MQ=255)
        cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAGCCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                          >  1:976871/1‑150 (MQ=255)
        cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                          >  1:862562/1‑150 (MQ=255)
        cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                          <  1:1106624/150‑1 (MQ=255)
         aGCGCATTCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACagag                                                                                                                                         >  2:829454/1‑150 (MQ=255)
              aTGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGTCTCTGTGCGGACATGCCCGCACagagtcctc                                                                                                                                    <  1:422809/150‑6 (MQ=255)
                          cGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtggctgg                                                                                                                        >  2:238594/1‑150 (MQ=255)
                              ccTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCAcc                                                                                                                    >  2:454380/1‑150 (MQ=255)
                                          gCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACAGAGTcctcga                                                                                                                                  >  2:863142/1‑120 (MQ=39)
                                          gCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATCCTATCGTCGAGCGCGGGATGCTGGGGGTGGGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCggg                                                                                                        >  2:204210/1‑149 (MQ=255)
                                                 gaagaaGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTcgcg                                                                                                 <  1:829454/150‑1 (MQ=255)
                                                     aagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGtt                                                                                             <  2:326440/150‑1 (MQ=255)
                                                            aGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCcgc                                                                                      <  1:50306/150‑1 (MQ=255)
                                                              gggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTTTGCCGGTGTTCGCGGGTTTACCCGCGa                                                                                    >  1:1028969/1‑150 (MQ=255)
                                                               ggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGt                                                                                              >  2:171749/1‑139 (MQ=255)
                                                               ggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGt                                                                                              <  1:171749/139‑1 (MQ=255)
                                                                       gCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCAc                                                                                                                     >  2:1011108/1‑108 (MQ=255)
                                                                       gCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCAc                                                                                                                     <  1:1011108/108‑1 (MQ=255)
                                                                        ccGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGc                                                                          <  2:862562/150‑1 (MQ=255)
                                                                         cggaaTCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCAAAGAGACCAACGCGGTCCATACCGAGATCGCTGACAGTGTTGTCGTTTTTACCCGCGAATACGGTAGCg                                                                         <  1:454380/146‑1 (MQ=255)
                                                                         cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACagag                                                                                                                                         >  2:131715/1‑86 (MQ=255)
                                                                         cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACagag                                                                                                                                         <  1:131715/86‑1 (MQ=255)
                                                                                gATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGCGAGAAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGcaa                                                                  >  1:509764/1‑150 (MQ=255)
                                                                                          tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                            >  2:207126/1‑66 (MQ=255)
                                                                                          tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                            <  1:207126/66‑1 (MQ=255)
                                                                                          tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGAAAGAATGAAcgg                                                        >  1:654510/1‑150 (MQ=255)
                                                                                            gAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcggcg                                                      >  1:539987/1‑150 (MQ=255)
                                                                                                     aTGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAATGGCGGGTGGAAAt                                             <  1:238594/150‑1 (MQ=255)
                                                                                                         tGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGc                                         >  2:1031599/1‑150 (MQ=255)
                                                                                                               tGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCcagcag                                   >  1:1019617/1‑150 (MQ=255)
                                                                                                                  gAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGggc                                <  1:1031599/150‑1 (MQ=255)
                                                                                                                         aCAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTAGCCAGCAGGGCCGGCctc                         >  1:857932/1‑150 (MQ=255)
                                                                                                                            gATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTc                      <  2:509764/150‑1 (MQ=255)
                                                                                                                                gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCgg                  >  2:943129/1‑150 (MQ=255)
                                                                                                                                     ctctGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaaa             >  1:713674/1‑150 (MQ=255)
                                                                                                                                      tctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCTAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGACATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc            >  1:718146/1‑150 (MQ=255)
                                                                                                                                       ctGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAcc           <  2:539987/150‑1 (MQ=255)
                                                                                                                                        tgtgCGGACATGCCCGCACAGAGGCCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAccc          >  2:88035/1‑150 (MQ=255)
                                                                                                                                              ggCATGTCCGCACAGAGACCAACGCGGTGGATGCGAGGTTCTCGGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt    <  2:1188580/150‑1 (MQ=21)
                                                                                                                                                cATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCCGCCGCAACAATGAACGGCGTGTGGAAATTGGCCAGCAGGGCCGGGCCCATAGCGTGAAAAACCGCTCCGAc  >  2:559404/1‑150 (MQ=25)
                                                                                                                                                    |                                                                                                                                                 
GCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC  >  NC_002947/3252392‑3252685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑