| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,397,592 | A→G | 9.5% | R10R (CGA→CGG) | PP_3002 → | shikimate dehydrogenase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,397,592 | 0 | A | G | 9.5% | 111.2 / 3.3 | 42 | R10R (CGA→CGG) | PP_3002 | shikimate dehydrogenase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base G (2/2); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
CCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAT > NC_002947/3397452‑3397738 | cccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAc > 1:582922/1‑150 (MQ=255)cccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAc < 2:313603/150‑1 (MQ=255)cccGGACCTTGGACCGCACCACGCAGTAGTGGCCGCCTTGCTTTGCTTCAGCCCGGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGTTCGGCCGACCGATCAAc < 2:615210/150‑1 (MQ=255) ccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAcc > 1:1031066/1‑150 (MQ=255) ccGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAAcc < 2:582922/150‑1 (MQ=255) cTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGTCGGCCGATCactgc < 1:1088115/145‑5 (MQ=255) cTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGTCGGCCGATCactgc > 2:1088115/1‑141 (MQ=255) ggCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGt < 2:172670/150‑1 (MQ=255) ccAGCACAACGCCGAAGTGGCCGCCGGGCTTAGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGCTCAACCACACCAAGTcc > 1:241813/1‑150 (MQ=255) gCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTc > 2:1024324/1‑150 (MQ=255) ttggcttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGc < 1:1024324/150‑1 (MQ=255) cttCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGc > 1:934933/1‑150 (MQ=255) cTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACcagca > 2:2208/1‑150 (MQ=255) gaagaaATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCagcagc > 1:666733/1‑150 (MQ=255) aagaaATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCt < 2:666733/150‑1 (MQ=255) agaaATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTg < 1:245740/150‑1 (MQ=255) aaaTCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGc < 2:481226/107‑1 (MQ=255) aaaTCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGc > 1:481226/1‑107 (MQ=255) tGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACggg > 1:970087/1‑150 (MQ=255) gCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAAc < 1:1077905/126‑1 (MQ=255) gCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAAc > 2:1077905/1‑126 (MQ=255) gCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATc < 1:2208/150‑1 (MQ=255) gCAGGCGGATGGCGGGGTGATTACCAAAGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGGCCCCGCTGATTCATGGCCTCTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATc < 2:241813/150‑1 (MQ=255) gCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCt < 1:376837/150‑1 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGaa > 1:1150537/1‑124 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGaa < 2:1150537/124‑1 (MQ=255) tgttgtTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGga < 2:934933/150‑1 (MQ=255) ttACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCACCCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACtt < 1:530647/150‑1 (MQ=255) tACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGt < 1:929848/88‑1 (MQ=255) tACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGt > 2:929848/1‑88 (MQ=255) ccACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACg < 2:825502/118‑1 (MQ=255) ccACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACg > 1:825502/1‑118 (MQ=255) cACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAg > 1:1111271/1‑150 (MQ=255) aCGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAAt < 2:49634/113‑1 (MQ=255) aCGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAAt > 1:49634/1‑113 (MQ=255) ggAGATGCGCGATGAGCGACCGCTACGCAGTGATCGGCCGACCGCTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGCCGACTTCGAAGccc > 2:146402/1‑150 (MQ=255) gaTGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAgg < 2:970087/150‑1 (MQ=255) gCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTAc > 1:283678/1‑150 (MQ=255) gCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCg > 2:787150/1‑150 (MQ=255) ggttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGaa < 2:137722/116‑1 (MQ=255) ggttGATCGGTCGGCCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGaa > 1:137722/4‑119 (MQ=255) gATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGaa < 2:1031066/150‑1 (MQ=255) ccgaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAt > 1:15553/1‑150 (MQ=255) gaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCa > 2:825995/1‑54 (MQ=255) gaccgaTCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCa < 1:825995/54‑1 (MQ=255) | CCCGGACCTTGGCCAGCACAACGCCGAAGTGGCCGCCGGGCTTGGCTTCAGCCCTGAAGAAATCACTGCCTTGCAGGCGGATGGCGTGTTGTTTACCCACGGAGATGCACGATGAGCGACCGCTACGCAGTGATCGGCCGACCGATCAACCACACCAAGTCCCCGCTGATTCATGGCCTGTTCGCCCAGGCCAGCAACCAGCAGCTGGAATACGGGGCTATCGAAGGCTCGCTGGACGACTTCGAAGCCCAGGTACTGCAGTTTCGTAGCGAAGGCGGGAAGGGCAT > NC_002947/3397452‑3397738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |