Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,485,143 G→A 8.6% P275L (CCG→CTG)  PP_3091 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,485,1430GA 8.6% 122.0 / 2.6 47P275L (CCG→CTG) PP_3091membrane protein
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

GGTGCTGTCGGCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTGGGCAGCC  >  NC_002947/3484998‑3485289
                                                                                                                                                 |                                                                                                                                                  
ggTGCTGTCGGCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGgctg                                                                                                                                                >  2:548937/1‑150 (MQ=255)
  tGCTGTCGGCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGgctgct                                                                                                                                              <  1:797540/150‑1 (MQ=255)
      gTCGGCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCg                                                                                                                                          >  1:941438/1‑150 (MQ=255)
        tggCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCAGCCGCTgggcat                                                                                                                                        <  1:1162297/149‑5 (MQ=255)
           cTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGgctg                                                                                                                                                <  2:450536/139‑1 (MQ=255)
           cTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGgctg                                                                                                                                                >  1:450536/1‑139 (MQ=255)
               ggCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCGCGGCGTGCAGGCAGTTGGATGGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAg                                                                                                                                 <  1:450611/150‑1 (MQ=255)
                     gAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAg                                                                                                                           <  2:364302/150‑1 (MQ=255)
                          cGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCgg                                                                                                                                                   <  1:1172300/121‑1 (MQ=255)
                          cGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCgg                                                                                                                                                   >  2:1172300/1‑121 (MQ=255)
                           gATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTc                                                                                                                     <  2:341820/150‑1 (MQ=255)
                                tcTGCGCGTCGACGTGCTGGTGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCAAGCGGCTGCTGGCGGTCGGTCAGGACGAGGACGTCGaaaa                                                                                                                >  2:498546/1‑149 (MQ=255)
                                    cgcgTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTg                                                                                                            <  1:548937/150‑1 (MQ=255)
                                     gcgTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTgg                                                                                                           <  1:690278/150‑1 (MQ=255)
                                           aCGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAg                                                                                                     <  1:555921/150‑1 (MQ=255)
                                                        tatGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTggt                                                                                        <  1:1206966/150‑1 (MQ=255)
                                                              cTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGt                                                                                                                                     <  2:597124/99‑1 (MQ=255)
                                                              cTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGt                                                                                                                                     >  1:597124/1‑99 (MQ=255)
                                                                      ttcttcAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGACCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTAGAAGTAGGCTGGCAACTGGTCGGTCTTGCTCAGc                                                                          >  2:755060/1‑150 (MQ=255)
                                                                       tcttcAGGCGCGTCTGCAGCACGGCGTGCAGTCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCa                                                                         >  2:1137076/1‑150 (MQ=255)
                                                                        cttcAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTc                                                                                                                     >  2:220756/1‑105 (MQ=255)
                                                                        cttcAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTc                                                                                                                     <  1:220756/105‑1 (MQ=255)
                                                                          tcAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCa                                                                      <  2:941438/150‑1 (MQ=255)
                                                                                gcgTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTaaa                                                                >  2:1015730/1‑150 (MQ=255)
                                                                                          caCGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGtt                                                      <  2:809583/150‑1 (MQ=255)
                                                                                           aCGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTg                                                     >  2:1194513/1‑150 (MQ=255)
                                                                                                 tGCAGGCCGTTGGTGCGGATTCCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGcc                                                                                                   >  1:1134764/1‑98 (MQ=255)
                                                                                                 tGCAGGCCGTTGGTGCGGATTCCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGcc                                                                                                   <  2:1134764/98‑1 (MQ=255)
                                                                                                   cAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAg                                             <  2:487585/150‑1 (MQ=255)
                                                                                                     ggCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGc                                           >  1:239429/1‑150 (MQ=255)
                                                                                                         gTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCAt                                         >  1:917403/1‑148 (MQ=255)
                                                                                                         gTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCAt                                         <  2:917403/148‑1 (MQ=255)
                                                                                                           tGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCgg                                     <  1:250361/150‑1 (MQ=255)
                                                                                                                     ttCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTAttcttc                           >  2:490043/1‑150 (MQ=255)
                                                                                                                         gAACGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGGTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCAcc                       >  2:323205/1‑150 (MQ=255)
                                                                                                                            aGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCgg                    <  1:1194513/150‑1 (MQ=255)
                                                                                                                            aGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCgg                    <  1:323205/150‑1 (MQ=255)
                                                                                                                               ccAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTc                                                                             >  1:220509/1‑90 (MQ=255)
                                                                                                                               ccAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTc                                                                             <  2:220509/90‑1 (MQ=255)
                                                                                                                               ccAAAGCTCATGCCCAGCGCCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAACGCCGGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCAGCGTATTCTTCCACCCGGGcc                 >  2:617925/1‑150 (MQ=255)
                                                                                                                                cAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGcca                >  1:931895/1‑150 (MQ=255)
                                                                                                                                      tCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCt          >  1:972659/1‑150 (MQ=255)
                                                                                                                                      tCAGGCCGAGCGGCTGCTGGCGTTCGTGCAGTTCGAGGCTGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCt          <  2:399917/150‑1 (MQ=255)
                                                                                                                                       gaacgccAGCAGCCGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTg         >  2:341952/6‑150 (MQ=255)
                                                                                                                                         acgccAGCAGCCGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGc                  <  2:1132729/136‑1 (MQ=255)
                                                                                                                                         acgccAGCAGCCGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGc                  >  1:1132729/4‑139 (MQ=255)
                                                                                                                                             cAGCTGCTGCTCGCGTTCGTGCAGGTCGAGGCCGGCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTGGGCAGc   <  2:239429/150‑1 (MQ=255)
                                                                                                                                             cAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTGGGCAGc   >  1:727982/1‑150 (MQ=255)
                                                                                                                                              aGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTGGGCAGcc  <  1:490043/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGTGCTGTCGGCTTCGGCACCGAGGGCGATCATCTGCGCGTCGACGTGCTGGCGAATATGAGCTTGCAGCTTCTTCAGGCGCGTCTGCAGCACGGCGTGCAGGCCGTTGGTGCGGATTTCCGAAAGGCCAAAGGTCATGCCCAGCGGCTGCTGGCGTTCGTGCAGGTCGAGGCCGTCGAAAGCCTGGCTGAAGCCCGGCAACTGGTCGGTCTTGCTCAGCATCAGGTAAACCGGCGGGTTGGCATCGAGGCATTCGGCGTATTCTTCCACCCGGGCCACCAGCTGGGCAGCC  >  NC_002947/3484998‑3485289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑