| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,584,942 | A→C | 13.1% | intergenic (+46/‑89) | benC → / → benD | benzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,584,942 | 0 | A | C | 13.1% | 103.4 / 11.8 | 46 | intergenic (+46/‑89) | benC/benD | benzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (21/19); new base C (2/4); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
TGTGCGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAGGCATC > NC_002947/3584793‑3585087 | tgtgCGGCCCGCCACCCATGGTGGAAGCGGGCAGCCAGTACGTGCGTAAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGa < 2:100936/150‑1 (MQ=255) cccGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCt > 1:155854/1‑150 (MQ=255) ccGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTc < 2:242083/150‑1 (MQ=255) ccGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTc < 1:202789/150‑1 (MQ=255) gCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCAGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTccc < 2:610110/150‑1 (MQ=255) ccACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCca > 1:800672/1‑150 (MQ=255) aCCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGc > 2:37883/1‑143 (MQ=255) aCCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGc < 1:37883/143‑1 (MQ=255) aTGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGa > 1:545453/1‑150 (MQ=255) aGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCa > 1:331475/1‑150 (MQ=255) cgtgcgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGa < 2:777049/150‑1 (MQ=255) tgcctgAGCAGGGCATTACCCCCGCCAACTTCTACTACGCGAAGTTTT‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGc < 2:800672/150‑1 (MQ=255) aGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAagaga < 2:545453/150‑1 (MQ=255) aGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGtt > 1:817404/1‑150 (MQ=255) gCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTtgct < 2:182960/150‑1 (MQ=255) ttACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGGTGCTGcc > 2:1201865/1‑150 (MQ=255) cccGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAg < 2:916246/150‑1 (MQ=255) cccGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAg > 1:1103818/1‑150 (MQ=255) cAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGtctc > 1:320904/1‑150 (MQ=255) tactacGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGaa < 1:229725/150‑1 (MQ=255) agtagaAGTTGGCCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg < 1:1094536/147‑1 (MQ=255) cGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGga < 2:320904/150‑1 (MQ=255) gagaAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGgag > 2:110569/1‑150 (MQ=255) tttG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTAc > 2:464676/1‑150 (MQ=255) tttG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTAc < 2:155854/150‑1 (MQ=255) cTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGa > 1:1009935/1‑150 (MQ=255) tCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg < 1:593995/120‑1 (MQ=255) tCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg > 2:593995/1‑120 (MQ=255) tCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGAtt > 2:629982/1‑126 (MQ=255) tCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGAtt < 1:629982/126‑1 (MQ=255) tCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaag > 1:437566/1‑150 (MQ=255) ccTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCcaaggc > 2:797918/1‑150 (MQ=255) aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGc > 1:365302/1‑150 (MQ=255) aTACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGt < 1:114893/150‑1 (MQ=255) ccGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCg < 2:817404/150‑1 (MQ=255) ccTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTgcc < 1:797918/150‑1 (MQ=255) ccTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTgcc < 1:1201865/150‑1 (MQ=255) cTGTGGGAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGtt < 1:1055287/67‑1 (MQ=37) cTGTGGGAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGtt > 2:1055287/1‑67 (MQ=37) ggAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCa > 1:882341/1‑136 (MQ=255) ggAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCa < 2:882341/136‑1 (MQ=255) ggAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCaa > 2:276890/1‑150 (MQ=255) gAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCTCAAg > 1:596523/1‑150 (MQ=255) aGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAgg > 1:877827/1‑150 (MQ=255) ggCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTAc < 2:1202357/91‑1 (MQ=255) ggCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTAc > 1:1202357/1‑91 (MQ=255) ggCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTTCCGCCCAAGGCGTc < 2:1103818/150‑1 (MQ=255) | TGTGCGGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTG‑CGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAAGGCATC > NC_002947/3584793‑3585087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |