| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,630,695 | T→A | 11.4% | W425R (TGG→AGG) | PP_3200 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,630,695 | 0 | T | A | 11.4% | 127.0 / 8.4 | 44 | W425R (TGG→AGG) | PP_3200 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/23); new base A (3/2); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01 | |||||||||||
ACTTCAACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTC > NC_002947/3630549‑3630844 | aCTTCAACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGc > 1:109909/1‑150 (MQ=255) ttCAACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCtt < 1:974263/150‑1 (MQ=255) aCCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCa > 1:187127/1‑150 (MQ=255) cGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGa < 1:848738/150‑1 (MQ=255) gAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCAGGCTGGCAAGCCAGGAGACg > 2:642912/1‑150 (MQ=255) ttGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAAc < 1:916168/150‑1 (MQ=255) tGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCAGGCTGGCAAGCCAGGAgacgttgg < 1:642912/150‑8 (MQ=255) gTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACtt > 2:449056/1‑150 (MQ=255) aaCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTACACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCAt > 1:572519/1‑150 (MQ=255) aCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGccag < 2:932558/135‑3 (MQ=255) aCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGccag > 1:932558/1‑133 (MQ=255) cTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGc > 2:474741/1‑150 (MQ=255) tGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACt > 2:263462/1‑139 (MQ=255) tGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACt < 1:263462/139‑1 (MQ=255) aGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGa < 2:815501/150‑1 (MQ=255) aGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGa > 1:286052/1‑150 (MQ=255) gCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAg > 2:269410/1‑150 (MQ=255) ccTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑Cg < 1:472179/150‑1 (MQ=255) caaccaGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAAt < 2:387102/150‑1 (MQ=255) gCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTg < 1:474741/150‑1 (MQ=255) aTGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCt > 1:1055334/1‑147 (MQ=255) aTGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCt < 2:1055334/147‑1 (MQ=255) tGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGaa > 2:1101297/1‑150 (MQ=255) ttGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCgg < 2:187127/150‑1 (MQ=255) ggCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCCGGAACCGGGt > 2:1016025/1‑150 (MQ=255) ggcgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc > 2:442381/1‑150 (MQ=255) ggcgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTTCTGCGGC‑TTATCGCCTGGAACCGGGTGATGAGc < 1:1124748/150‑1 (MQ=255) cgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGt < 2:286052/150‑1 (MQ=255) atgcgaTCGACAATGACTATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGAGTGa < 1:48348/148‑1 (MQ=255) aggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGATTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGa < 2:4996/150‑1 (MQ=255) cGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGaa < 1:1101297/150‑1 (MQ=255) aaTGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc > 1:420930/1‑135 (MQ=255) aaTGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc < 2:420930/135‑1 (MQ=255) aaCCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCCGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGtt < 1:1016025/150‑1 (MQ=255) aaCCCGGTTTATGCCACCGGCCGCACGCTACACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGtt < 2:572519/150‑1 (MQ=255) ggCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGt > 1:681277/1‑150 (MQ=255) gCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCt > 2:368383/1‑76 (MQ=255) gCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCt < 1:368383/76‑1 (MQ=255) ccGCACCCTGCACGACAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGa < 1:213804/150‑1 (MQ=255) cGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGAc < 1:94192/150‑1 (MQ=255) tGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGccacca < 2:109909/150‑1 (MQ=255) cACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGc < 1:442381/150‑1 (MQ=255) cAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTgcg > 1:865653/1‑150 (MQ=255) ctcCAGGCTGGCAAGCCAGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTggg > 2:1118817/1‑149 (MQ=255) tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc < 1:720677/81‑1 (MQ=255) tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc > 2:720677/1‑81 (MQ=255) tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACTCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTc > 2:1144796/1‑150 (MQ=255) tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTc > 2:22927/1‑150 (MQ=255) | ACTTCAACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTC > NC_002947/3630549‑3630844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |