Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,630,703 C→A 9.8% A427A (GCC→GCA)  PP_3200 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,630,7030CA 9.8% 156.0 / 6.0 51A427A (GCC→GCA) PP_3200hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base A (3/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

ACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTCGAGCCG  >  NC_002947/3630555‑3630850
                                                                                                                                                    |                                                                                                                                                    
aCCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCa                                                                                                                                                     >  1:187127/1‑150 (MQ=255)
       cGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGa                                                                                                                                              <  1:848738/150‑1 (MQ=255)
           gAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCAGGCTGGCAAGCCAGGAGACg                                                                                                                                          >  2:642912/1‑150 (MQ=255)
              ttGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAAc                                                                                                                                       <  1:916168/150‑1 (MQ=255)
               tGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCAGGCTGGCAAGCCAGGAgacgttgg                                                                                                                                      <  1:642912/150‑8 (MQ=255)
                gTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACtt                                                                                                                                     >  2:449056/1‑150 (MQ=255)
                   aaCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTACACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCAt                                                                                                                                  >  1:572519/1‑150 (MQ=255)
                    aCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGccag                                                                                                                                                <  2:932558/135‑3 (MQ=255)
                    aCCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCAGGCTGGCAAGccag                                                                                                                                                >  1:932558/1‑133 (MQ=255)
                         cTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGc                                                                                                                            >  2:474741/1‑150 (MQ=255)
                          tGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACt                                                                                                                                      >  2:263462/1‑139 (MQ=255)
                          tGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACt                                                                                                                                      <  1:263462/139‑1 (MQ=255)
                                 aGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGa                                                                                                                    >  1:286052/1‑150 (MQ=255)
                                 aGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGa                                                                                                                    <  2:815501/150‑1 (MQ=255)
                                  gCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAg                                                                                                                   >  2:269410/1‑150 (MQ=255)
                                          ccTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑Cg                                                                                                          <  1:472179/150‑1 (MQ=255)
                                                 caaccaGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAAt                                                                                                   <  2:387102/150‑1 (MQ=255)
                                                       gCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTg                                                                                             <  1:474741/150‑1 (MQ=255)
                                                         aTGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCt                                                                                              <  2:1055334/147‑1 (MQ=255)
                                                         aTGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCt                                                                                              >  1:1055334/1‑147 (MQ=255)
                                                          tGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGaa                                                                                          >  2:1101297/1‑150 (MQ=255)
                                                              ttGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCgg                                                                                      <  2:187127/150‑1 (MQ=255)
                                                                ggCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCCGGAACCGGGt                                                                                    >  2:1016025/1‑150 (MQ=255)
                                                                       ggcgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc                                                                             >  2:442381/1‑150 (MQ=255)
                                                                       ggcgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTTCTGCGGC‑TTATCGCCTGGAACCGGGTGATGAGc                                                                             <  1:1124748/150‑1 (MQ=255)
                                                                         cgaggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGt                                                                           <  2:286052/150‑1 (MQ=255)
                                                                           atgcgaTCGACAATGACTATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGGCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGAGTGa                                                                         <  1:48348/148‑1 (MQ=255)
                                                                           aggcgaTCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGATTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGa                                                                         <  2:4996/150‑1 (MQ=255)
                                                                                  cGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGaa                                                                  <  1:1101297/150‑1 (MQ=255)
                                                                                      aaTGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc                                                                             <  2:420930/135‑1 (MQ=255)
                                                                                      aaTGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc                                                                             >  1:420930/1‑135 (MQ=255)
                                                                                               aaCCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCCGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGtt                                                     <  1:1016025/150‑1 (MQ=255)
                                                                                               aaCCCGGTTTATGCCACCGGCCGCACGCTACACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGtt                                                     <  2:572519/150‑1 (MQ=255)
                                                                                                                 ggCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGt                                   >  1:681277/1‑150 (MQ=255)
                                                                                                                  gCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCt                                                                                                             <  1:368383/76‑1 (MQ=255)
                                                                                                                  gCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCt                                                                                                             >  2:368383/1‑76 (MQ=255)
                                                                                                                   ccGCACCCTGCACGACAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGa                                 <  1:213804/150‑1 (MQ=255)
                                                                                                                    cGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGAc                                <  1:94192/150‑1 (MQ=255)
                                                                                                                           tGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGccacca                         <  2:109909/150‑1 (MQ=255)
                                                                                                                             cACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGc                       <  1:442381/150‑1 (MQ=255)
                                                                                                                                  cAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTgcg                  >  1:865653/1‑150 (MQ=255)
                                                                                                                                        ctcCAGGCTGGCAAGCCAGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTggg            >  2:1118817/1‑149 (MQ=255)
                                                                                                                                            tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc                                                                             <  1:720677/81‑1 (MQ=255)
                                                                                                                                            tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGc                                                                             >  2:720677/1‑81 (MQ=255)
                                                                                                                                            tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACTCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTc        >  2:1144796/1‑150 (MQ=255)
                                                                                                                                            tGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTc        >  2:22927/1‑150 (MQ=255)
                                                                                                                                             ggCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGt                                   <  1:231205/122‑1 (MQ=255)
                                                                                                                                             ggCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGt                                   >  2:231205/1‑122 (MQ=255)
                                                                                                                                              gCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAACAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTCGa      >  1:624546/1‑150 (MQ=255)
                                                                                                                                                 tGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTCGAGcc   >  1:214462/1‑150 (MQ=255)
                                                                                                                                                  gCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTCGAGCCg  >  2:279820/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCTCGCCGGTGAGTTGTCAACCCGCTGGAACCAGCCGTTGGCCTCGACCAACCAGCATGCGTTGGCCGTGGGCGAGGCGATCGACAATGACGATAACCCGGTTTATGCCACCGGCCGCACGCTGCACGCCAACGTCTCCTGGCTTGCCAGCCTGGAGCCGAACTTCATCGCTGCCGAGTCGAGCTTCCT‑CGGCGAAATCGCCTGGAACCGGGTGATGAGCGTGAGCAAGAACGCCGATGCCGTTGACCCTAACGCCGACCGTGACGCCACCAGCCTGCGCCTGGTGTTCGAGCCG  >  NC_002947/3630555‑3630850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑