Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,673,285 A→T 8.3% D97V (GAC→GTC)  PP_3237 → universal stress protein family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,673,2850AT 8.3% 139.6 / 5.2 48D97V (GAC→GTC) PP_3237universal stress protein family protein
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base T (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CTGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCAAC  >  NC_002947/3673143‑3673433
                                                                                                                                              |                                                                                                                                                    
cTGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTg                                                                                                                                               <  1:224850/150‑1 (MQ=255)
cTGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTg                                                                                                                                               <  1:1020623/150‑1 (MQ=255)
cTGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTg                                                                                                                                               <  2:155236/150‑1 (MQ=255)
 tGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACAATCAGCAGCAACAGCTGGAAACACAGGCGAACGCGCTGCGTAGCTCCTGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTgg                                                                                                                                              >  2:37585/1‑150 (MQ=255)
   gAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGaa                                                                                                                                            <  1:195909/150‑1 (MQ=255)
   gAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGaa                                                                                                                                            <  1:265383/150‑1 (MQ=255)
            aaGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATg                                                                                                                                   <  1:268638/150‑1 (MQ=255)
             aGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATgg                                                                                                                                  <  1:761915/150‑1 (MQ=255)
                 ccAAACGGGGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGgagac                                                                                                                              <  2:961114/150‑2 (MQ=255)
                                  cTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCtgctgc                                                                                                             <  1:831531/150‑1 (MQ=255)
                                   ttACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTgctgct                                                                                                            >  1:590146/1‑150 (MQ=255)
                                             acTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGAc                                                                                                                         >  1:388369/1‑127 (MQ=255)
                                             acTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGAc                                                                                                                         <  2:388369/127‑1 (MQ=255)
                                               tCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGa                                                                                                >  2:503436/1‑150 (MQ=255)
                                               tCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGa                                                                                                >  1:755604/1‑150 (MQ=255)
                                               tCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGa                                                                                                >  2:231134/1‑150 (MQ=255)
                                                           gCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCCGGGCGAGCATGAGCCcgc                                                                                    >  2:796696/1‑150 (MQ=255)
                                                             tGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCgcgc                                                                                  >  1:387003/1‑150 (MQ=255)
                                                             tGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCgcgc                                                                                  <  1:415448/150‑1 (MQ=255)
                                                                aaaCCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCa                                                                               >  2:313099/1‑150 (MQ=255)
                                                                       ggCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTTGAgcgcgg                                                                        >  1:89805/1‑149 (MQ=255)
                                                                        tcGAACGCGCTGCGTGCCTCCGACGTCCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCAACGTAAACGTCATGGCGACCCGCAAGGACGGGGAGGCAGTGCTGATCGAGCATGAGCCCGCGCTCAAGCGCGcc                                                                       <  1:510525/149‑1 (MQ=255)
                                                                           aaCGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCcttc                                                                    <  1:696722/150‑1 (MQ=255)
                                                                                   gCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCAcccc                                                            >  1:773299/1‑150 (MQ=255)
                                                                                          tCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGAc                                                     <  1:503436/150‑1 (MQ=255)
                                                                                                      gTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCATCACCCCGCTTGACTGGAAACTGTTg                                         >  1:850816/1‑150 (MQ=255)
                                                                                                             ccGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTTGAgcgcggg                                                                       >  1:312695/1‑111 (MQ=255)
                                                                                                             ccGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTTGAgcgcggg                                                                       <  2:312695/113‑3 (MQ=255)
                                                                                                                       gTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGCCTGGAAACTGTTGCGCCa                                    >  1:907701/1‑138 (MQ=255)
                                                                                                                       gTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCa                                    <  2:907701/138‑1 (MQ=255)
                                                                                                                        tGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGcccggtcccg                       <  2:929056/150‑1 (MQ=255)
                                                                                                                         ggAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTTCGCCACTGcccggtcccgg                      <  1:37585/150‑1 (MQ=255)
                                                                                                                             cAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGATGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTa                  >  1:934060/1‑150 (MQ=255)
                                                                                                                              aaTGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTAc                 <  2:355208/150‑1 (MQ=255)
                                                                                                                                   ccTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCt            >  1:19028/1‑150 (MQ=255)
                                                                                                                                    ttccaagATGTCATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGcccggtc                          <  2:748119/129‑1 (MQ=255)
                                                                                                                                    ttccaagATGTCATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGcccggtc                          >  1:748119/7‑135 (MQ=255)
                                                                                                                                    ttccAGGATGTCATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGcccggtcccggt                     <  2:228077/137‑1 (MQ=255)
                                                                                                                                    ttccAGGATGTCATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGcccggtcccggt                     >  1:228077/4‑140 (MQ=255)
                                                                                                                                    cTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTc           >  1:576799/1‑150 (MQ=255)
                                                                                                                                     tGCAGGGTGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCg          >  1:810789/1‑150 (MQ=255)
                                                                                                                                     tGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCAAACCGCTTGAATGGAAAATGATGCGCCACTGCCCGGTCCCGGTCTACCTGCtgg          >  2:918612/1‑148 (MQ=255)
                                                                                                                                       cAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGATCTGCCGTTGAAGGAGGTCGAGCATGAGCCAACCATGAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCggg        <  1:270795/150‑1 (MQ=255)
                                                                                                                                            tGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCaa   <  2:755604/150‑1 (MQ=255)
                                                                                                                                            tGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCaa   >  1:206169/1‑150 (MQ=255)
                                                                                                                                            tGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCaa   <  2:989945/150‑1 (MQ=255)
                                                                                                                                            tGACATCCTGGAACACGTCATGGAGATGCGACAGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCaa   <  1:492891/150‑1 (MQ=255)
                                                                                                                                             gACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCAAc  <  2:19028/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CTGGAGTCCGGTAAGCACCAAACGGAGCGGGAGGCTTACCGGCACACTCAGCAGCAACAGCTGGAAACCCAGGCGAACGCGCTGCGTGCCTCCGGCGTGCAGGTGACGACCGAGGTGGCGTGGAGCAATGACCTGCAGGATGACATCCTGGAACACGTCATGGAGATGCGACCGGACCTGCTGCTCAAGCAGGTCGAGCATGAGCCCGCGCTCAAGCGCGCCTTCTTCACCCCGCTTGACTGGAAACTGTTGCGCCACTGCCCGGTCCCGGTCTACCTGCTCGGGGCCAAC  >  NC_002947/3673143‑3673433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑