| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,691,282 | G→T | 8.3% | L194I (CTC→ATC) | PP_3257 ← | putative Methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,691,282 | 0 | G | T | 8.3% | 182.6 / 5.5 | 60 | L194I (CTC→ATC) | PP_3257 | putative Methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (25/30); new base T (3/2); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
AGCGGTCCAGTACCACCAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAGGGTGC > NC_002947/3691133‑3691424 | aGCGGTCCAGTACCACCAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCgag > 2:452974/1‑150 (MQ=255) ggTCCAGTACCACCAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATc > 2:832307/1‑150 (MQ=255) accaccAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCGGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGATCTcgacgaga > 1:148855/1‑147 (MQ=255) accaccAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGATCTcgacgaga < 2:148855/149‑3 (MQ=255) caccaGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCtt < 2:250338/150‑1 (MQ=255) ccaGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTtgg < 1:135096/150‑1 (MQ=255) ccaGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTtgg > 1:366544/1‑150 (MQ=255) caGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGAAGACGCTtggc > 1:129198/1‑150 (MQ=255) aGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTtggct > 2:1106536/1‑150 (MQ=255) cTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCa < 1:452974/150‑1 (MQ=255) ttCCCCGTGCAGACCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCAGCGGGGATGGCCATGTTCGGGGTGCGTCGTTATGTGGATGAATGACAACGGCCATCATGGCCAGGTCTCGTCGAGATCGACGACGCTTGGCTGGCAt < 1:1178508/150‑1 (MQ=255) ttCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCAt > 2:977346/1‑150 (MQ=255) ccACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGa < 2:546924/150‑1 (MQ=255) cACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGAc > 2:463963/1‑150 (MQ=255) cACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGATTCGTTGTGTGCCGGGATGACAACGACAGTCATGGTCACGGCTCGTCGAGATCGACGACGCTTGGCTGGCATGAc > 2:738358/1‑150 (MQ=255) aCGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTtggct < 2:898088/141‑1 (MQ=255) aCGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTtggct > 1:898088/1‑141 (MQ=255) cGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCa < 2:722969/150‑1 (MQ=255) tCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTcc < 2:1059844/150‑1 (MQ=255) cccGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCa < 1:302645/150‑1 (MQ=255) ccGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAg > 1:1203625/1‑150 (MQ=255) gctgcGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCaaaaa > 2:128517/1‑150 (MQ=255) ccTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCCTATCGCTATTCCAGTGGTGGGTGGCATGACCAGTATTCCAGCAAAAAGTCCGc < 1:1114386/150‑1 (MQ=255) cGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGcctcct < 2:810318/150‑1 (MQ=255) caccaGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGa < 1:832307/150‑1 (MQ=255) ccaGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGaaa < 1:3185/150‑1 (MQ=255) gCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAActg > 1:199891/1‑150 (MQ=255) cACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAActgc < 2:129198/150‑1 (MQ=255) aCCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAActgct < 1:128517/150‑1 (MQ=255) aCCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAActgct > 2:1110002/1‑150 (MQ=255) aCCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAActgct < 2:366544/150‑1 (MQ=255) gcAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCaa < 1:977346/150‑1 (MQ=255) gCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGAt > 2:711231/1‑73 (MQ=255) gCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGAt < 1:711231/73‑1 (MQ=255) aTCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCa < 2:559824/104‑1 (MQ=255) aTCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCa > 1:559824/1‑104 (MQ=255) cAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCg < 1:1068095/150‑1 (MQ=255) ggcggcGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGt < 2:199891/150‑1 (MQ=255) gcggGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAAt < 1:1084166/150‑1 (MQ=255) gCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGc < 1:738358/150‑1 (MQ=255) cTTCGTTATGTGCAGGGATGACACCGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCg > 1:1158711/1‑150 (MQ=255) cTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCg > 1:280993/1‑150 (MQ=255) tgCAGGGATGACAACGGCAATCATGGTCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTcg > 2:488875/1‑150 (MQ=255) tgCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTcg > 2:146544/1‑150 (MQ=255) tgCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTcg > 2:474493/1‑150 (MQ=255) gggATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGAc < 2:1203625/150‑1 (MQ=255) gggATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGAc > 2:511565/1‑150 (MQ=255) ggATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGACCGCCTCCTCAt > 1:704586/1‑92 (MQ=255) ggATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGACCGCCTCCTCAt < 2:704586/92‑1 (MQ=255) aaCGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGc > 1:747569/1‑150 (MQ=255) ggCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCggg < 1:704794/150‑1 (MQ=255) ggCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCggg > 2:571820/1‑150 (MQ=255) aaTCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCaa < 2:747569/150‑1 (MQ=255) cATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAAccc > 2:929593/1‑150 (MQ=255) aTGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCcg < 2:1158711/150‑1 (MQ=255) cGTCTCGTCGAGACCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAgg < 1:204999/150‑1 (MQ=255) tcgtcgATCTCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAggg > 1:126448/1‑147 (MQ=255) tcgtcgATCTCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAggg < 2:126448/147‑1 (MQ=255) tcgtcgATCTCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAGGGTGc > 1:424785/1‑150 (MQ=255) tcgtcgAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAAt < 2:24632/102‑1 (MQ=255) tcgtcgAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAAt > 1:24632/1‑102 (MQ=255) gagaTCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTc > 2:295776/1‑111 (MQ=255) gagaTCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTc < 1:295776/111‑1 (MQ=255) | AGCGGTCCAGTACCACCAGCACTTCCACGTGCAGTCCCGCTTGCTGCGCCTGCCGGGCAGCCACCAGCACCGCCCGCAGGCATCGCCCAAGGCGGCGGGCTTCGTTATGTGCAGGGATGACAACGGCAATCATGGGCACGTCTCGTCGAGATCGACGACGCTTGGCTGGCATGACCAGTATTCCAGCAAAAAGTCCGCCTCCTCATGCCGAAACTGCTGATGCAACGGCAAATGTCGGGTCAATAGGCGGTGCACTTCGCGACCGTCCTGCGGGCAACCCGCGATAGGGTGC > NC_002947/3691133‑3691424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |