| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,711,792 | A→G | 7.3% | Y78H (TAT→CAT) | paaB ← | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,711,792 | 0 | A | G | 7.3% | 157.5 / 2.7 | 55 | Y78H (TAT→CAT) | paaB | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
GGCCACACCATTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATC > NC_002947/3711646‑3711939 | ggCCACACCATTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGAc < 1:512925/150‑1 (MQ=255) ccACACCATTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCt < 2:1104820/150‑1 (MQ=255) cATTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTgtcgt < 1:100493/150‑1 (MQ=255) aTTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTgtcgtc < 1:360753/150‑1 (MQ=255) ttCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTcc > 2:78694/1‑150 (MQ=255) gcTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGaa < 2:955917/150‑1 (MQ=255) tGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACa < 1:1199598/150‑1 (MQ=255) gCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGc < 1:931202/150‑1 (MQ=255) ccTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCgg < 1:692738/150‑1 (MQ=255) cTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCggg < 1:794775/150‑1 (MQ=255) ggACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTc > 2:530535/1‑150 (MQ=255) ccAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCttt < 2:623596/150‑1 (MQ=255) cgcTGTCGCCGGGTAACAGCAGGGAGGGGAGAAGGCCTTCTTTATGCACGGCCCACCTCACATGTGTCCGACTTCGTCCGGCAGCTCGTAGAACCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCtcgtc < 1:557202/150‑1 (MQ=255) cTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGCCgg > 2:275052/1‑150 (MQ=255) aCAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCgg < 2:228388/137‑1 (MQ=255) aCAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCgg > 1:228388/1‑137 (MQ=255) aCAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCAAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGt > 2:197203/1‑150 (MQ=255) gcagGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCg > 1:178698/1‑119 (MQ=255) gcagGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCg < 2:178698/119‑1 (MQ=255) gTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAgc > 2:978719/1‑150 (MQ=255) gTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAgc > 2:471062/1‑150 (MQ=255) gATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGaa < 1:530535/150‑1 (MQ=255) ggCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGcacc > 2:839977/1‑150 (MQ=255) ttcttTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCaccacc > 2:334833/1‑150 (MQ=255) ttATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCcaca < 1:839977/150‑1 (MQ=255) aTGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCAAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGa < 1:197203/150‑1 (MQ=255) gCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCacac > 1:173584/1‑150 (MQ=255) ccACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGTGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCt > 1:686775/1‑150 (MQ=255) cacaTGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTc < 1:1091305/83‑1 (MQ=255) cacaTGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTc > 2:1091305/1‑83 (MQ=255) acaTGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATGGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTAc > 2:271368/1‑150 (MQ=255) acaTGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATGGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTAc > 2:271736/1‑150 (MQ=255) aTGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGTGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTAcgg > 2:580924/1‑150 (MQ=255) tgtCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTAcggcgg < 2:173584/150‑1 (MQ=255) gtCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCGCACATTCGGTAcgacagc > 2:858583/1‑145 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGc < 1:355258/70‑1 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGc > 2:355258/1‑70 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGaa < 1:536018/112‑1 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGaa > 2:536018/1‑112 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGgt < 1:517290/150‑1 (MQ=255) tCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGgt > 1:751339/1‑150 (MQ=255) gACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCTAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCa < 1:626899/101‑1 (MQ=255) gACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCa > 2:626899/1‑101 (MQ=255) ggCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGTGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCgg < 1:580924/150‑1 (MQ=255) gCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGt > 1:761800/1‑150 (MQ=255) aGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGa > 1:999439/1‑150 (MQ=255) aaGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATg < 2:761800/150‑1 (MQ=255) aaGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATg < 1:847122/150‑1 (MQ=255) gCATGGCGATGGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATg < 1:271736/150‑1 (MQ=255) gCATGGCGATGGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATg < 1:271368/150‑1 (MQ=255) gCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATg > 2:543671/1‑150 (MQ=255) aTGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATg < 1:849552/142‑1 (MQ=255) aTGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATg > 2:849552/1‑142 (MQ=255) tGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATggcg < 1:543671/150‑1 (MQ=255) aTAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATc > 1:762149/1‑150 (MQ=255) | GGCCACACCATTCGCAGAGGCGCTGGCCCTGGACCAGGGCGCTGTCGCCGAGCAACAGCAGGTAGGGGATAAGGGCTTCTTTATGCATGGCCCACCTCACATGTGTCCGACTTCGTCGGGCAGCTCGTAGAAGCTGGCATGGCGATAGACCTTGTCGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATC > NC_002947/3711646‑3711939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |