| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,716,177 | G→C | 13.7% | T52S (ACC→AGC) ‡ | paaI ← | hydroxyphenylacetyl‑CoA thioesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,716,177 | 0 | G | C | 13.7% | 135.5 / 14.5 | 51 | T52S (ACC→AGC) ‡ | paaI | hydroxyphenylacetyl‑CoA thioesterase |
| Reads supporting (aligned to +/- strand): ref base G (23/21); new base C (4/3); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
GGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGT > NC_002947/3716037‑3716317 | gggCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAACAATGTGCTACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGcc < 2:766570/150‑1 (MQ=255) cGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCt > 1:5363/1‑150 (MQ=255) ggTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTg < 1:622508/150‑1 (MQ=255) aTCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTTGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGc > 2:587629/1‑150 (MQ=255) cgcgCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGACGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCAc > 1:878866/1‑150 (MQ=255) gcgcAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATgg < 1:1200363/130‑2 (MQ=255) gcgcAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATgg < 1:1200521/130‑2 (MQ=255) gcgcAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATgg > 2:1200363/1‑129 (MQ=255) gcgcAGCGCCGGGGCAAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCACCTGCCATgg > 2:1200521/1‑129 (MQ=255) agggacGGGGCCAGGTAGTCGATGGTTCAGCACAGCGCGACTGTGGCTTGGTCGTAGCTATTGCAGGCGAAGGCGAGGGCCGAATCGGTCGCCGCGGATCGGAAGCCGCCATGCCAGGTGCCATTCTCGTGGAACATTTCGGCACGTAcc < 2:104167/145‑1 (MQ=255) cGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCAt > 2:1153606/1‑150 (MQ=255) cAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAg > 2:681425/1‑150 (MQ=255) tCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcc > 2:701610/1‑150 (MQ=255) gggTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccggc < 2:803525/149‑1 (MQ=255) tGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATTATTTCGGCACGTACCGCCATGCGCAGGCTGgcccggcccg > 1:615935/1‑150 (MQ=255) gCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGcccggcccgg > 1:307076/1‑150 (MQ=255) cAGCGCCACTGTGGCCTGGTGGTAGCTATTGCAGGCGAAGGCTGACGCCGAATCGGCTAACGCGAAAAGGAAACCGCCATGGAAGGTGCCATCCCCGCGTATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGGCCGGGCCAGc < 2:203784/150‑1 (MQ=255) gcgcCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCAt > 2:249979/1‑150 (MQ=255) ccACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca < 2:844526/150‑1 (MQ=255) ggCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCt < 2:1910/150‑1 (MQ=255) cttgTCGTAGCTCTTGCAGGCGAGGGAGGAGGTCTTGTCGATGGACGGGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCTGCATCCAGCAGGCTGAt < 2:615935/147‑1 (MQ=255) cTGGTCGTAGCTATTGCAGTCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGACTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGAt > 1:553762/1‑150 (MQ=255) cTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCAt > 1:56477/1‑108 (MQ=255) cTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCAt < 2:56477/108‑1 (MQ=255) cTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGTGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGAt > 1:35849/1‑150 (MQ=255) cTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGAt > 1:912425/1‑150 (MQ=255) gTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca > 2:485868/1‑134 (MQ=255) gTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCca < 1:485868/134‑1 (MQ=255) ttGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTgg < 1:681425/150‑1 (MQ=255) ttGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTgg < 2:23126/150‑1 (MQ=255) gCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGTGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGt < 2:35849/150‑1 (MQ=255) cgaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgc < 1:609051/150‑1 (MQ=255) gaaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgcg > 1:1029974/1‑150 (MQ=255) aaCGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATACAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCgg < 1:1153606/150‑1 (MQ=255) tCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATATCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATg < 1:1150248/150‑1 (MQ=255) cAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTc > 2:201197/1‑150 (MQ=255) gcgAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGca > 1:307320/1‑150 (MQ=255) ggAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCtt < 2:878866/150‑1 (MQ=255) gAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCCGCATCCAGCATGCCGATACCCCGGCCCTGGGTGGCCTGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtg > 2:540207/1‑150 (MQ=255) aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt > 2:376188/1‑150 (MQ=255) aaaCCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTtgt > 2:376092/1‑150 (MQ=255) gggcATGGCACCTGCCATGGCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCggg < 1:772360/110‑1 (MQ=255) gggcATGGCACCTGCCATGGCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCggg > 2:772360/4‑113 (MQ=255) ggcATGGCACCTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATCAGGAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAg > 1:12429/3‑150 (MQ=255) gCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCCGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgc > 1:85165/1‑116 (MQ=255) gCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTcgc < 2:85165/116‑1 (MQ=255) ccATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGACGGCATAATGCAGGCTGCTACCCAGGCCCGGGGTGGGCGGGTGGTGGTCGTATATGGCTTCGGCACAGGCCTGTGccgac < 2:740526/150‑4 (MQ=255) aTGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCg > 2:602963/1‑70 (MQ=255) aTGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCg < 1:602963/70‑1 (MQ=255) tGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccggc < 2:138995/61‑1 (MQ=255) tGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTggcccggc > 1:138995/1‑61 (MQ=255) | GGGCGGTGAGCACGTCATCGCGCAGCGCCGGGGCCAGGTAGTCGATGGTGCAGCCCAGCGCCACCGTGGCCTGGTCGTAGCTATTGCAGGCGAAGGCGAACGCCGAATCGGCCAACGCGAACAGGAAACCGCCATGGCAGGTGCCATGCCCCTGGATCATGTCGGCACGTACCGCCATGCGCAGGCTGGCCCGGCCCGGGCCGGCATCCAGCAGGCTGATACCCAGGCCCTGGGTGGCCGGGTCGCGGGCGTACATGGCGTCGGCACAGGCTTGTGCCAGT > NC_002947/3716037‑3716317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |