Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,762,985	A→T	13.6%	N62I (AAC→ATC)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,762,985	0	A	T	13.6%	169.4 / 15.2	67	N62I (AAC→ATC)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base T (3/6);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAT  >  NC_002947/3762836‑3763130                                                                                                                                                      |                                                                                                                                                  ccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACTTGACCGGGaa                                                                                                                                                   >  1:486669/1‑150 (MQ=255) ccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGaa                                                                                                                                                   >  1:79787/1‑150 (MQ=255)      tGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGa                                                                                                                                              >  1:1179615/1‑150 (MQ=255)         tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGCACATGATcc                                                                                                                                           >  2:230754/1‑150 (MQ=255)         tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTcc                                                                                                                                           >  1:438699/1‑150 (MQ=255)           aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGCGGTTGCGGTTAACATGATCCCg                                                                                                                                         <  2:1200029/150‑1 (MQ=255)           aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACACGGTCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGTGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCg                                                                                                                                         <  1:74762/150‑1 (MQ=255)              gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTc                                                                                                                                      >  1:602350/1‑150 (MQ=255)               tgaaCGCAGGCGCCCAAGGGCTGTGGACCGAGAAGCGACGTGGCAATATCGAGGCCGGGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGGGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCGGTCa                                                                                                                                     <  2:1131967/148‑1 (MQ=255)               tCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCa                                                                                                                                     >  2:296171/1‑150 (MQ=255)               tCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCa                                                                                                                                     >  2:344842/1‑150 (MQ=255)                cAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCGGTCAc                                                                                                                                    <  2:438699/150‑1 (MQ=255)                  aCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATTCCGGTCACgg                                                                                                                                  >  1:119931/1‑150 (MQ=255)                            ccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCt                                                                                                                        >  2:1121940/1‑150 (MQ=255)                              aCGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTgg                                                                                                                      >  1:926954/1‑150 (MQ=255)                                ggCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCg                                                                                                                    >  2:1206276/1‑150 (MQ=255)                                  ccTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGa                                                                                                                  >  1:501247/1‑150 (MQ=255)                                    tgtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCACAAGTATGATCCCGGTCACGGTCGAGCGCCTGGCGGATc                                                                                                                >  1:62760/1‑150 (MQ=255)                                           cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCAt                                                                                                                                                <  2:1158361/111‑1 (MQ=255)                                           cGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCAt                                                                                                                                                >  1:1158361/1‑111 (MQ=255)                                             aggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgc                                                                                                       >  1:598671/1‑150 (MQ=255)                                               gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGTCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctg                                                                                                     >  1:1149063/1‑150 (MQ=255)                                                  cGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCaa                                                                                                  <  2:119931/150‑1 (MQ=255)                                                   atcttGGCAATATAGAGGGCGTGTACGCACACGGCGCTAAGTATAACTCCTACAACGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAAc                                                                                                 <  2:132031/146‑1 (MQ=255)                                                     cGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCa                                                                                               >  2:628362/1‑150 (MQ=255)                                                       tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACt                                                                                             >  1:1201361/1‑150 (MQ=255)                                                        ggCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCagat                                                                                            >  1:132031/1‑147 (MQ=255)                                                          cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAc                                                                                          >  2:520544/1‑150 (MQ=255)                                                           aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGAGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                         >  2:644024/1‑150 (MQ=255)                                                             tatCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcccc                                                                                       <  2:1099138/150‑1 (MQ=255)                                                               tCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCTGc                                                                                     <  1:1206276/150‑1 (MQ=255)                                                                 gAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACAt                                                                                                                                                >  1:651869/1‑89 (MQ=255)                                                                 gAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACAt                                                                                                                                                <  2:651869/89‑1 (MQ=255)                                                                    gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                <  2:501247/150‑1 (MQ=255)                                                                     ccGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTTCCCCGCCTGCgg                                                                               <  2:79787/150‑1 (MQ=255)                                                                          tcgggCCACGGCGCCGCCGATAACGCCTCCAAGGCAGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGACCATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                          <  2:554429/147‑1 (MQ=255)                                                                          tACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                          <  1:520544/150‑1 (MQ=255)                                                                             ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGcc                                                                       <  2:567237/150‑1 (MQ=255)                                                                              gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTgaga                                                                      >  1:554429/1‑147 (MQ=255)                                                                              gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                      >  1:813863/1‑150 (MQ=255)                                                                               ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                         >  1:102534/1‑130 (MQ=255)                                                                               ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                         <  2:102534/130‑1 (MQ=255)                                                                                cACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtg                                                                    >  1:516334/1‑150 (MQ=255)                                                                                        cGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                                            >  1:406945/1‑150 (MQ=255)                                                                                        cGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGAGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                                            >  1:1073380/1‑150 (MQ=255)                                                                                         gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGAc                                                           >  2:928759/1‑150 (MQ=255)                                                                                         gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGAc                                                           >  2:29405/1‑150 (MQ=255)                                                                                            gATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGAGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAAc                                                        <  1:344842/150‑1 (MQ=255)                                                                                                   ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                 <  2:813863/150‑1 (MQ=255)                                                                                                   ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCAGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                 <  2:1179615/150‑1 (MQ=255)                                                                                                    cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                <  1:1005963/150‑1 (MQ=255)                                                                                                      aCAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                              <  2:1073380/150‑1 (MQ=255)                                                                                                       cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTc                                             <  1:763467/150‑1 (MQ=255)                                                                                                          ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                          <  2:516334/150‑1 (MQ=255)                                                                                                           gCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAg                                         <  2:1138382/150‑1 (MQ=255)                                                                                                              gAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcgc                                      >  2:782352/1‑150 (MQ=255)                                                                                                               aaaaGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGcc                                     <  1:29405/150‑1 (MQ=255)                                                                                                                      cAAGCGCGCCTGGGCGTTCGACGTGACCGCGATCATGATCGCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                              <  2:62760/150‑1 (MQ=255)                                                                                                                        aGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGc                            >  1:154644/1‑150 (MQ=255)                                                                                                                             gcCTGGGCGTTCGACGTGACCGGGAAGATGATCCCGGTCACGGTCGAGCGCGTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCGTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                       <  2:475137/150‑1 (MQ=255)                                                                                                                             gcCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                       <  1:296171/150‑1 (MQ=255)                                                                                                                                    cGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATc                <  1:782352/150‑1 (MQ=255)                                                                                                                                        gaccGTGACCGGGATCATGTTCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                              <  1:766269/129‑1 (MQ=255)                                                                                                                                        gaccGTGACCGGGATCATGTTCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                              >  2:766269/4‑132 (MQ=255)                                                                                                                                        gaccGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGa            >  2:1141119/4‑150 (MQ=255)                                                                                                                                               aCCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGACTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAcc     >  1:37231/1‑150 (MQ=255)                                                                                                                                                  gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCAc                                                                            <  2:441025/76‑1 (MQ=255)                                                                                                                                                  gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCAc                                                                            >  1:441025/1‑76 (MQ=255)                                                                                                                                                  gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAt  <  2:926954/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAT  >  NC_002947/3762836‑3763130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑