Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,762,990	A→T	9.5%	I64F (ATC→TTC)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,762,990	0	A	T	9.5%	178.0 / 9.6	63	I64F (ATC→TTC)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/26);  new base T (2/4);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

TGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGA  >  NC_002947/3762841‑3763138                                                                                                                                                      |                                                                                                                                                     tGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGa                                                                                                                                                      >  1:1179615/1‑150 (MQ=255)    tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGCACATGATcc                                                                                                                                                   >  2:230754/1‑150 (MQ=255)    tCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTcc                                                                                                                                                   >  1:438699/1‑150 (MQ=255)      aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGCGGTTGCGGTTAACATGATCCCg                                                                                                                                                 <  2:1200029/150‑1 (MQ=255)      aGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACACGGTCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGTGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCg                                                                                                                                                 <  1:74762/150‑1 (MQ=255)         gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTc                                                                                                                                              >  1:602350/1‑150 (MQ=255)          tgaaCGCAGGCGCCCAAGGGCTGTGGACCGAGAAGCGACGTGGCAATATCGAGGCCGGGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGGGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCGGTCa                                                                                                                                             <  2:1131967/148‑1 (MQ=255)          tCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCa                                                                                                                                             >  2:296171/1‑150 (MQ=255)          tCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCa                                                                                                                                             >  2:344842/1‑150 (MQ=255)           cAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGATCATGTTCCCGGTCAc                                                                                                                                            <  2:438699/150‑1 (MQ=255)             aCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATTCCGGTCACgg                                                                                                                                          >  1:119931/1‑150 (MQ=255)                       ccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCt                                                                                                                                >  2:1121940/1‑150 (MQ=255)                         aCGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTgg                                                                                                                              >  1:926954/1‑150 (MQ=255)                           ggCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCg                                                                                                                            >  2:1206276/1‑150 (MQ=255)                             ccTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGa                                                                                                                          >  1:501247/1‑150 (MQ=255)                               tgtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCACAAGTATGATCCCGGTCACGGTCGAGCGCCTGGCGGATc                                                                                                                        >  1:62760/1‑150 (MQ=255)                                        aggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgc                                                                                                               >  1:598671/1‑150 (MQ=255)                                          gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGTCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctg                                                                                                             >  1:1149063/1‑150 (MQ=255)                                             cGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCaa                                                                                                          <  2:119931/150‑1 (MQ=255)                                              atcttGGCAATATAGAGGGCGTGTACGCACACGGCGCTAAGTATAACTCCTACAACGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAAc                                                                                                         <  2:132031/146‑1 (MQ=255)                                                cGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCa                                                                                                       >  2:628362/1‑150 (MQ=255)                                                  tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACt                                                                                                     >  1:1201361/1‑150 (MQ=255)                                                   ggCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCagat                                                                                                    >  1:132031/1‑147 (MQ=255)                                                     cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAc                                                                                                  >  2:520544/1‑150 (MQ=255)                                                      aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGAGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                                 >  2:644024/1‑150 (MQ=255)                                                        tatCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcccc                                                                                               <  2:1099138/150‑1 (MQ=255)                                                          tCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCTGc                                                                                             <  1:1206276/150‑1 (MQ=255)                                                               gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                        <  2:501247/150‑1 (MQ=255)                                                                ccGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTTCCCCGCCTGCgg                                                                                       <  2:79787/150‑1 (MQ=255)                                                                     tcgggCCACGGCGCCGCCGATAACGCCTCCAAGGCAGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGACCATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                                  <  2:554429/147‑1 (MQ=255)                                                                     tACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACtt                                                                                  <  1:520544/150‑1 (MQ=255)                                                                        ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGcc                                                                               <  2:567237/150‑1 (MQ=255)                                                                         gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTgaga                                                                              >  1:554429/1‑147 (MQ=255)                                                                         gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                                              >  1:813863/1‑150 (MQ=255)                                                                          ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                                 <  2:102534/130‑1 (MQ=255)                                                                          ccACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                                 >  1:102534/1‑130 (MQ=255)                                                                           cACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtg                                                                            >  1:516334/1‑150 (MQ=255)                                                                                   cGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                                                    >  1:406945/1‑150 (MQ=255)                                                                                   cGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGAGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                                                    >  1:1073380/1‑150 (MQ=255)                                                                                    gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGAc                                                                   >  2:29405/1‑150 (MQ=255)                                                                                    gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGAc                                                                   >  2:928759/1‑150 (MQ=255)                                                                                       gATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGAGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAAc                                                                <  1:344842/150‑1 (MQ=255)                                                                                              ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                         <  2:813863/150‑1 (MQ=255)                                                                                              ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCAGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                         <  2:1179615/150‑1 (MQ=255)                                                                                               cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                        <  1:1005963/150‑1 (MQ=255)                                                                                                 aCAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                                      <  2:1073380/150‑1 (MQ=255)                                                                                                  cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTc                                                     <  1:763467/150‑1 (MQ=255)                                                                                                     ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                                  <  2:516334/150‑1 (MQ=255)                                                                                                      gCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAg                                                 <  2:1138382/150‑1 (MQ=255)                                                                                                         gAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcgc                                              >  2:782352/1‑150 (MQ=255)                                                                                                          aaaaGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGcc                                             <  1:29405/150‑1 (MQ=255)                                                                                                                 cAAGCGCGCCTGGGCGTTCGACGTGACCGCGATCATGATCGCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                                      <  2:62760/150‑1 (MQ=255)                                                                                                                   aGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGc                                    >  1:154644/1‑150 (MQ=255)                                                                                                                        gcCTGGGCGTTCGACGTGACCGGGAAGATGATCCCGGTCACGGTCGAGCGCGTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCGTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                               <  2:475137/150‑1 (MQ=255)                                                                                                                        gcCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                               <  1:296171/150‑1 (MQ=255)                                                                                                                               cGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATc                        <  1:782352/150‑1 (MQ=255)                                                                                                                                   gaccGTGACCGGGATCATGTTCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                                      <  1:766269/129‑1 (MQ=255)                                                                                                                                   gaccGTGACCGGGATCATGTTCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACaa                                      >  2:766269/4‑132 (MQ=255)                                                                                                                                   gaccGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGa                    >  2:1141119/4‑150 (MQ=255)                                                                                                                                          aCCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGACTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAcc             >  1:37231/1‑150 (MQ=255)                                                                                                                                             gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCAc                                                                                    >  1:441025/1‑76 (MQ=255)                                                                                                                                             gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCAc                                                                                    <  2:441025/76‑1 (MQ=255)                                                                                                                                             gggAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCAt          <  2:926954/150‑1 (MQ=255)                                                                                                                                                     gATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGa  >  1:1197070/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGACGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGA  >  NC_002947/3762841‑3763138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑