Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,774,290 C→G 12.8% A317G (GCC→GGC)  PP_5743 → TonB‑dependent receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,774,2900CG12.8% 110.4 / 9.1 39A317G (GCC→GGC) PP_5743TonB‑dependent receptor protein
Reads supporting (aligned to +/- strand):  ref base C (15/19);  new base G (3/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

AACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGCA  >  NC_002947/3774147‑3774430
                                                                                                                                               |                                                                                                                                            
aaCGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGGCAGCCt                                                                                                                                        >  2:700393/1‑150 (MQ=255)
   gCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGGCAGCCTGac                                                                                                                                     <  1:700393/150‑2 (MQ=255)
                         gATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATCTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTTGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCAt                                                                                                               <  1:777103/150‑1 (MQ=255)
                          aTGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGAACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATg                                                                                                              <  2:394002/150‑1 (MQ=255)
                                  ggtggtGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGCCAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAg                                                                                                      >  1:219787/1‑150 (MQ=255)
                                   gtggtgCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGGCAGCCTGacgg                                                                                                                                   <  2:217825/120‑4 (MQ=255)
                                   gtggtgCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGGCAGCCTGacgg                                                                                                                                   >  1:217825/1‑117 (MQ=255)
                                      gtgCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAca                                                                                                  <  1:1021111/150‑1 (MQ=255)
                                         cGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTAGCCGGCGTAAGGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatcc                                                                                               <  1:1047703/150‑1 (MQ=255)
                                           gTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCtt                                                                                                          <  2:930010/137‑1 (MQ=255)
                                           gTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCtt                                                                                                          >  1:930010/1‑137 (MQ=255)
                                           gTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatccat                                                                                             >  2:946244/1‑150 (MQ=255)
                                           gTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatccat                                                                                             >  1:1153867/1‑150 (MQ=255)
                                               aGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCAtcgtc                                                                                         <  2:1080344/150‑1 (MQ=255)
                                                 cAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatccat                                                                                             <  2:282577/144‑1 (MQ=255)
                                                 cAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTAcatccat                                                                                             >  1:282577/1‑144 (MQ=255)
                                                        aTGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTc                                                                                <  1:66921/150‑1 (MQ=255)
                                                            gCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGt                                                                            <  1:311541/150‑1 (MQ=255)
                                                            gCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGt                                                                            <  2:133671/150‑1 (MQ=255)
                                                              aGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTg                                                                          <  2:835933/150‑1 (MQ=255)
                                                                gTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCa                                                                                                                              <  1:1137067/96‑1 (MQ=255)
                                                                gTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCa                                                                                                                              >  2:1137067/1‑96 (MQ=255)
                                                                gTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGa                                                                        >  2:1139004/1‑150 (MQ=255)
                                                                      cTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTgg                                                                  <  2:1153867/150‑1 (MQ=255)
                                                                             ttCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCGTCGTCGGTAACCTCATGTGGGAGCTTGGGCAAAgg                                                           <  2:318397/150‑1 (MQ=255)
                                                                                 aCAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGc                                                       >  2:281453/1‑150 (MQ=255)
                                                                                  cAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTgg                                                                  <  1:1161122/138‑1 (MQ=255)
                                                                                  cAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGATTgg                                                                  >  2:1161122/1‑138 (MQ=255)
                                                                                          gggAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGAcggc                                              >  1:1128072/1‑150 (MQ=255)
                                                                                                 gCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGcggccggc                                       <  2:219787/150‑1 (MQ=255)
                                                                                                           gtggtgTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAAc                             >  1:1040024/1‑150 (MQ=255)
                                                                                                                     ggTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGt                                                         >  2:126443/1‑112 (MQ=255)
                                                                                                                     ggTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGt                                                         <  1:126443/112‑1 (MQ=255)
                                                                                                                         ttCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGtt                                                                           >  2:294477/1‑90 (MQ=255)
                                                                                                                         ttCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGtt                                                                           <  1:294477/90‑1 (MQ=255)
                                                                                                                             gCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCgg           <  1:965517/150‑1 (MQ=255)
                                                                                                                             gCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCgg           >  1:334143/1‑150 (MQ=255)
                                                                                                                                    ccGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCgg    >  1:898619/1‑150 (MQ=255)
                                                                                                                                      ttCAGGCTGGCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGca  >  1:793880/2‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                            
AACGCTTGCCGGCTTCGGTCAGCGCGATGCGTCGGGTGGTGCGGTTGAGCAGGCGAATGTGCAGGTGAGCCTCCAGGTTCGACAGCATCCGGGAAACGCTGGCCGTGGTGGTGTCCAGGTATTCGGCCGCACCCGTCAGGCTGCCAGCCTGAACCACGCAGTTGAACGCGCGCATGGCTTGAAGTACATCCATCGTCGGTAACCTCATGTTGGAGCTTGGGCAAAGGGTGCAAGGACGGCGGCCGGCCACCGTCAACTGATCATGGTTTAGCCGGGCACCGGCA  >  NC_002947/3774147‑3774430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑